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公开(公告)号：US06344601B1
公开(公告)日：2002-02-05
申请号：US09061897
申请日：1998-04-17
IPC分类号： C12N1582
CPC分类号： C12N15/827 , C12N15/8261 , Y02A40/146
摘要： Plant growth habit is altered by causing either under-expression or over-expression of profilin in a plant cell. Under-expression of profilin can be achieved by transforming a plant or plant cell with a gene expressing an antisense mRNA complementary to the mRNA transcribed by the coding sequence of a profilin gene and expressing the gene in the plant or plant cell such that the antisense mRNA inhibits the production of the profilin in the plant or plant cell. Under-expression of profilins in plants can lead to such alterations in growth habit as a dwarf phenotype, a reduced root and root hair system, and delay in the onset of flowering. Over-expression of profilin can be achieved by transforming a plant or plant cell with a gene capable of expressing a profilin in the plant or plant cell and causing the transformed gene to be expressed in the plant or plant cell. Over-expression of profilin in a plant can lead to such alterations in growth habit as a tall phenotype, an expansion of the root and root hair system, expansion of leaf surface area and accelerating the onset of flowering.
摘要翻译： 通过在植物细胞中引起profilin的低表达或过度表达来改变植物生长习性。 profilin的低表达可以通过用表达与由profilin基因的编码序列转录的mRNA互补的反义mRNA的基因转化植物或植物细胞并在植物或植物细胞中表达该基因来实现，使得反义mRNA 抑制植物细胞或植物细胞中profilin的产生。 植物中profilins的低表达可导致生长习性如矮矮化表型，根系和根系减少延迟以及开花发生的延迟。 通过用能够在植物或植物细胞中表达profilin的基因转化植物或植物细胞并使转化的基因在植物或植物细胞中表达，可以实现profilin的过表达。 植物中profilin的过度表达可导致生长习性如高表型，根系和根系发育扩张，叶表面积扩大和加速开花的发生。
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2.发明授权LexA-based chemical-inducible gene expression system for use in transgenic animals 有权基于LexA的化学诱导型基因表达系统用于转基因动物公开(公告)号：US08852928B2
公开(公告)日：2014-10-07
申请号：US12663323
申请日：2008-07-02
CPC分类号： C12N15/85 , A01K67/0275 , A01K2217/052 , A01K2217/15 , A01K2217/203 , A01K2227/40 , A01K2267/03 , A01K2267/0393 , C07K2319/71 , C07K2319/715 , C07K2319/80 , C12N15/8509 , C12N2800/40 , C12N2830/002
摘要： The present invention relates generally to chemical-inducible system and to methods of use in transgenic animals. More specifically, the present invention relates to a chimeric transcription factor that binds to a ligand and functions in ligand-dependent manner to induce expression of genes of interest under the control of a synthetic operator-promoter sequence. The expression of genes of interest can be tightly controlled by adding or removing the ligand.
摘要翻译： 本发明一般涉及化学诱导系统和转基因动物中使用的方法。 更具体地，本发明涉及结合配体并以配体依赖性方式起作用以在合成的操作者 - 启动子序列的控制下诱导感兴趣的基因表达的嵌合转录因子。 感兴趣的基因的表达可以通过添加或除去配体来严格控制。
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3.发明授权公开(公告)号：US07816580B2
公开(公告)日：2010-10-19
申请号：US12722981
申请日：2010-03-12
申请人： Jianru Zuo , Qi-Wen Niu , Giovanna Frugis , Nam-Hai Chua
发明人： Jianru Zuo , Qi-Wen Niu , Giovanna Frugis , Nam-Hai Chua
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , A01H4/005 , C12N15/821 , C12N15/8287 , Y02A40/146
摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.
摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。
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公开(公告)号：US20100186125A1
公开(公告)日：2010-07-22
申请号：US12448263
申请日：2007-12-14
申请人： Simon Moller , Nam-Hai Chua
发明人： Simon Moller , Nam-Hai Chua
CPC分类号： C12N15/8214
摘要： The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.
摘要翻译： 本发明涉及一种生产转化植物细胞的方法。 更具体地，该方法涉及用靶向植物质体的转化盒转化植物细胞，其包含选择基因，例如异戊烯基转移酶（IPT）和转基因。 在选择转化质体后，在植物细胞中诱导重组酶的表达，这导致选择基因从质体中切除和转化子在质体中的表达。 本发明还提供包含转化盒的细胞和植物。
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公开(公告)号：US06441276B1
公开(公告)日：2002-08-27
申请号：US09964850
申请日：2001-09-28
申请人： Yoshihisa Ikeda , Nam-Hai Chua
发明人： Yoshihisa Ikeda , Nam-Hai Chua
IPC分类号： C12N1587
CPC分类号： C12N15/821 , C07K14/415 , C12N15/8295
摘要： A plant gene, Esr2, has been found which when overexpressed in plant cells results in cells which have cytokinin-independent cell growth. This feature allows the encoded protein ESR2 to be used as a selectable marker of transformed cells by growing the transformed cells on cytokinin-free media. It has also been found that overexpression of ESR2 in cells grown in the presence of cytokinins results in a higher regeneration of plants. This feature allows the gene to be used to obtain greater regeneration of plant cells.
摘要翻译： 已经发现植物基因Esr2在植物细胞中过表达时产生具有细胞分裂素依赖性细胞生长的细胞。 该特征允许编码的蛋白质ESR2通过在不含细胞分裂素的培养基上生长转化的细胞而用作转化细胞的选择标记。 还已经发现，在细胞分裂素存在下生长的细胞中ESR2的过表达导致植物更高的再生。 该特征允许该基因用于获得更大的植物细胞再生。
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公开(公告)号：US6083687A
公开(公告)日：2000-07-04
申请号：US888367
申请日：1992-05-26
IPC分类号： C07K14/415 , C12Q1/68
CPC分类号： C07K14/415 , C07K2319/02
摘要： A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.
摘要翻译： 通过聚合酶链反应（PCR），使用对应于作为引物的两个区域的混合寡核苷酸和作为模板的三叶草胶体cDNA文库，分离编码水解蛋白的cDNA克隆（HEV1）。 HEV1长1018个核苷酸，包括204个氨基酸的开放阅读框。 推测的氨基酸序列含有17个氨基酸残基的随机信号序列，随后是187个氨基酸的多肽。 氨基末端区域（43个氨基酸）与外源蛋白相同，并显示与几丁质结合蛋白质和马铃薯和杨树中伤口诱导基因的氨基末端的同源性。 多肽的羧基末端部分（144个氨基酸）与马铃薯的伤口诱导基因的羧基末端区域同源74-79％。 伤口以及植物激素脱落酸和乙烯的应用导致叶片，茎和胶乳中hevein转录物的积累，但不能在根中积累，如使用cDNA作为探针所示。 在本发明的蛋白质的大肠杆菌中产生融合蛋白，由大肠杆菌产生的麦芽糖结合蛋白。
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公开(公告)号：US20110162114A1
公开(公告)日：2011-06-30
申请号：US12997744
申请日：2009-06-12
申请人： Simon Geir Moller , Nam-Hai Chua , Jodi Maple
发明人： Simon Geir Moller , Nam-Hai Chua , Jodi Maple
IPC分类号： A01H5/00 , C12N5/10 , C12Q1/04 , C12P21/00 , C12N9/00 , C12N15/63 , C07K14/00 , C07K2/00 , C07K16/00
CPC分类号： C12N15/8214
摘要： The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.
摘要翻译： 本发明涉及一种生产转化植物细胞的方法。 更具体地，该方法涉及用靶向植物质体的转化盒转化植物细胞，其包含选择基因，例如异戊烯基转移酶（IPT）和转基因。 在选择转化质体后，在植物细胞中诱导重组酶的表达，这导致选择基因从质体中切除和转化子在质体中的表达。 本发明还提供包含转化盒的细胞和植物。
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公开(公告)号：US20100293662A1
公开(公告)日：2010-11-18
申请号：US12223929
申请日：2006-02-13
申请人： Lianghui Ji , Lin Cai , Nam-Hai Chua
发明人： Lianghui Ji , Lin Cai , Nam-Hai Chua
IPC分类号： C12N15/82 , C12N15/05 , C07H21/04 , C12N1/21 , C12N5/10 , C07K14/415 , C07K14/00 , C40B20/04 , C12N5/02
CPC分类号： C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/821 , C12N15/8261 , Y02A40/146
摘要： A group of genes including GhCIR1 from cotton (Gossypium hirsutum), and AtCIR1 and AtCIR2 from Arabidopsis thaliana promote shoot regeneration in plants even in the absence of cytokinin. In the presence of cytokinin, the genes significantly improve transformation efficiency. The genes can be used as an enhancer as well as a selectable marker of transformation in plants. The proteins encoded by the novel genes have a homeodomain (HD) at the N-terminus and a highly divergent domain at the C-terminus. The proteins share a common structural motif.
摘要翻译： 包括来自棉花（Gossypium hirsutum）的GhCIR1和来自拟南芥的AtCIR1和AtCIR2的一组基因即使在不存在细胞分裂素的情况下也促进植物中的枝条再生。 在细胞分裂素的存在下，基因显着提高转化效率。 这些基因可以用作植物中的增强子以及转化的选择标记。 由新基因编码的蛋白质在N末端具有同源结构域（HD），在C-末端具有高度不同的结构域。 蛋白质具有共同的结构基序。
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公开(公告)号：US07525012B2
公开(公告)日：2009-04-28
申请号：US10196416
申请日：2002-07-17
申请人： Jianru Zuo , Qiwen Niu , Nam-Hai Chua
发明人： Jianru Zuo , Qiwen Niu , Nam-Hai Chua
CPC分类号： C12N15/8295 , C12N15/8209 , C12N15/821 , C12N15/8238 , C12N15/8242
摘要： Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.
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10.发明授权Chemical inducible promoter used to obtain transgenic plants with a silent marker and organisms and cells and methods of using same for screening for mutations 失效用于获得具有沉默标记和生物体和细胞的转基因植物的化学诱导型启动子以及使用它们筛选突变的方法公开(公告)号：US07230157B1
公开(公告)日：2007-06-12
申请号：US10129849
申请日：2000-11-13
申请人： Jianru Zuo , Nam-Hai Chua
发明人： Jianru Zuo , Nam-Hai Chua
CPC分类号： C12N15/8274 , C12N15/8209 , C12N15/8237 , C12N15/8238 , C12N15/8242 , C12N15/8295
摘要： Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.
摘要翻译： 公开了一种化学诱导型启动子，用于通过将植物或细胞添加到含有诱导物的培养基中或通过从这种培养基中除去可调节基因来转化植物或植物细胞。 启动子可通过糖皮质激素，雌激素或对植物无内源性的诱导物诱导。 这样的启动子可以与任何植物基因一起使用，所述植物基因可以在糖皮质激素，雌激素或诱导物的存在下促进芽再生和发育以诱导芽形成。 启动子可以与抗生素或除草剂抗性基因或其它可通过给定诱导物的存在或不存在而调节的基因一起使用。 还提供了包含基因的生物体或细胞，其中基因的天然启动子被破坏并且将基因置于转基因诱导型启动子的控制下。 这些生物和细胞及其后代可用于筛选功能条件增加和功能突变丧失。
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