公开(公告)号：US11169071B2

公开(公告)日：2021-11-09

申请号：US16340055

申请日：2017-10-04

Applicant: National University Corporation Nagoya University ,  OSAKA UNIVERSITY ,  KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION

Inventor： Hirotoshi Yasaki ,  Takao Yasui ,  Noritada Kaji ,  Yoshinobu Baba ,  Tomoji Kawai ,  Satoyuki Kawano ,  Kentaro Doi ,  Takeshi Yanagida ,  Mao Fukuyama

IPC: G01N15/00 ,  G01N27/447 ,  G01N15/10 ,  G01N27/327

Abstract: In a method for analyzing samples involving the use of a device for analyzing samples, the device for analyzing samples includes at least a movement part through which a sample moves, and a measurement unit that is formed in a middle of the movement part and that measures a value of an ion current when the sample passes through the movement part. The analysis method includes at least a measurement step for measuring the value of the ion current when the sample passes through the movement part, and a determination step for determining a change over time in a quantity of ions from the value of the ion current measured in the measurement step. The quantity of ions includes a quantity of ions that have leaked from the sample during movement of the sample through the movement part.

公开(公告)号：US20160138101A1

公开(公告)日：2016-05-19

申请号：US14883494

申请日：2015-10-14

Applicant: Osaka University

Inventor： Masateru Taniguchi ,  Makusu Tsutsui ,  Kazumichi Yokota ,  Tomoji Kawai

IPC: C12Q1/68 ,  G01N27/327

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  G01N27/3278 ,  C12Q2565/631 ,  C12Q2565/607 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2563/157

Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.

Abstract translation: 本发明提供了使用当前测量来鉴定核苷酸并确定多核苷酸中的核苷酸序列的技术。 本发明计算当用于分析的核苷酸或多核苷酸通过电极之间时产生的隧道电流的模态值，然后采用所计算的模态值。 因此，本发明使得能够直接快速实施鉴定核苷酸并确定多核苷酸中的核苷酸序列，而无需标记。

公开(公告)号：US20160319342A1

公开(公告)日：2016-11-03

申请号：US15061871

申请日：2016-03-04

Applicant: Osaka University ,  Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G01N27/04 ,  G06F19/22

CPC classification number: C12Q1/6869 ,  G01N27/04 ,  G01N33/48721 ,  G06F19/22 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

Abstract translation: 提供了用于测序蛋白质样品的设备，系统和方法。 在一些实例中，当在几个不同距离中的每一个测量单体通过纳米缝电极对的电极之间产生的电流，使得当与已知单体的参考物理量相比时，识别单体，其可以从 以类似的电极间距测量的电流，其中可以以预定精度识别和排序多种单体中的每一种，并且基于从隧道电流获得的检测到的物理量，其可以通过使用一个 或更多的参考物质。

公开(公告)号：US20150219593A1

公开(公告)日：2015-08-06

申请号：US14421809

申请日：2013-08-02

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji Kawai ,  Takahito Ohshiro ,  Masateru Taniguchi

IPC: G01N27/447

CPC classification number: G01N27/447 ,  G01N15/1031 ,  G01N27/3275 ,  G01N27/4473 ,  G01N33/48721 ,  G01N2015/1006

Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.

Abstract translation: 分析方法允许分析具有高灵敏度的样品，而不考虑电极间距离。 该方法包括：在与样品的迁移方向相交的方向形成电场的第一电极对之间施加电压的步骤; 在第一电极对之间放置包括在电极对产生氧化还原反应的电化学活性分子的溶液的步骤; 使样品迁移的步骤; 以及测量第一电极对之间的电流变化量的步骤。

公开(公告)号：US20140183040A1

公开(公告)日：2014-07-03

申请号：US13975610

申请日：2013-08-26

Applicant: Osaka University

Inventor： Tomoji Kawai ,  Soh Ryuzaki ,  Masateru Taniguchi

IPC: G01N27/447

CPC classification number: G01N27/44756 ,  G01N27/44752 ,  G01N33/48721

Abstract: The present invention provides a method and apparatus for controlling the moving speed of a substance, both of which can adjust the moving speed of a substance to a desired speed. The control method and control apparatus cause a substance to pass through an internal space, in which an electro-osmotic flow is generated, of a surround electrode formed so as to surround part of the moving path of the substance, whereby the control method and control apparatus change the moving speed of the substance.

Abstract translation: 本发明提供了一种用于控制物质的移动速度的方法和装置，它们都可以将物质的移动速度调节到期望的速度。 控制方法和控制装置使物质通过形成为围绕物质的移动路径的一部分而形成的环境电极的产生电渗流的内部空间，由此控制方法和控制 设备改变物质的移动速度。

公开(公告)号：US20170306396A1

公开(公告)日：2017-10-26

申请号：US15242221

申请日：2016-08-19

Applicant: Osaka University

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro

IPC: C12Q1/68 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721

Abstract: The nano-gap electrode pair 12 is disposed so that a biomolecule joined to at least one or more types of a single molecule included in a sample passes an opposing position, and the strength of the electric field in a position spaced only a predetermined distance on the downstream side from the opposing position 64 becomes stronger than the strength of the electric field in a position spaced only the predetermined distance on the upstream side from the opposing position 64.

公开(公告)号：US09726636B2

公开(公告)日：2017-08-08

申请号：US14484305

申请日：2014-09-12

Applicant: KABUSHIKI KAISHA TOSHIBA ,  OSAKA UNIVERSITY

Inventor： Sadato Hongo ,  Tomoji Kawai ,  Makusu Tsutsui ,  Masateru Taniguchi ,  Soh Ryuzaki

IPC: G01N27/447 ,  G01N15/12 ,  G01N33/487 ,  G01N15/10

CPC classification number: G01N27/44756 ,  G01N15/12 ,  G01N15/1218 ,  G01N33/48721 ,  G01N2015/1062 ,  G01N2015/1087 ,  G01N2015/1093

Abstract: According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t

公开(公告)号：US20170146511A1

公开(公告)日：2017-05-25

申请号：US15344199

申请日：2016-11-04

Applicant: Osaka University

Inventor： Masateru Taniguchi ,  Tomoji Kawai ,  Takahito Ohshiro

IPC: G01N33/487 ,  C12Q1/68 ,  G01N27/447

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/44791

Abstract: The present disclosure provides devices, systems and methods for effectuating nanoelectrodes for use with determining the sequence of double stranded biopolymers. Various modified bases and different metals may be utilized alone or in combination so as to provide differentiation between different nucleobases and to determine which base is associated with which strand.

公开(公告)号：US20160245790A1

公开(公告)日：2016-08-25

申请号：US15048889

申请日：2016-02-19

Applicant: Osaka University ,  Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masayuki Furuhashi ,  Masateru Kawaguchi ,  Mark Oldham ,  Eric Nordman

IPC: G01N33/487 ,  G01N27/26 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N33/48721 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0663 ,  B01L2200/10 ,  B01L2300/04 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/086 ,  C12Q1/6813 ,  C12Q1/6869 ,  G01N27/26 ,  G01N27/3278 ,  C12Q2527/107 ,  C12Q2527/137 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: The present disclosure provides methods and systems that can reduce the amount of sample necessary to detect or identify, or both detect and identify, a biomolecule, and increase the rate of denaturing of the biomolecule. A device for thermally denaturing a biomolecule may include: a substrate having low thermal conductivity; a heater disposed adjacent to the substrate; a temperature sensor disposed adjacent to the substrate; a semiconductor oxide film disposed adjacent to the substrate, a nanochannel formed in a region of the semiconductor oxide film, and a cover over the nanochannel.

Abstract translation: 本公开提供了可以减少检测或识别或既检测和鉴定生物分子又增加生物分子的变性速率所需的样品量的方法和系统。 用于热变性生物分子的装置可以包括：具有低导热性的基底; 靠近所述基板设置的加热器; 设置在所述基板附近的温度传感器; 邻近衬底设置的半导体氧化膜，形成在半导体氧化膜的区域中的纳米通道以及纳米通道上的覆盖层。