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1.发明授权Continuous process for the separation of biologic components from heterogeneous cell populations 失效用于从异种细胞群体分离生物成分的连续过程
公开(公告)号：US5916743A
公开(公告)日：1999-06-29
申请号：US467818
申请日：1995-06-06
申请人： William C. Lake , Richard Giesler , Dennis Van Epps , John R. Chapman , Jeffrey A. Martinson , Dale R. Ellis , Frederick Aono , Daniel F. Bischof
发明人： William C. Lake , Richard Giesler , Dennis Van Epps , John R. Chapman , Jeffrey A. Martinson , Dale R. Ellis , Frederick Aono , Daniel F. Bischof
IPC分类号： A61M1/36 , C12N5/00 , C12N5/02 , C12N5/06 , C12Q1/24 , G01N33/50 , G01N33/569 , A01N1/02 , C12N1/02 , C12Q1/68
CPC分类号： G01N33/56972 , A61M1/362 , A61M1/3696 , C12Q1/24 , G01N33/5002 , G01N33/5005 , G01N33/569 , Y10T436/111666 , Y10T436/25375 , Y10T436/2575
摘要： A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.
摘要翻译： 提供了结合免疫亲和分离和连续流离心分离技术的方法，用于从异质细胞混合物中选择性分离成核的异质细胞群。 非均相细胞混合物紧密接触以促进与附着有活性结合特定所需类型的细胞的细胞混合物的颗粒结合。 选择颗粒使得颗粒/细胞共轭体的沉降速度与细胞混合物中的其它细胞的沉降速度充分不同，以允许其通过连续流动细胞分离器分离。 该方法以预期的免疫亲和力分离的高精度快速处理大量的细胞混合物，并且可用于分离例如来自全血，骨髓浓缩物或外周血干细胞浓缩物的各种类型的白细胞; 或淋巴因子活化的杀伤细胞的前体，肿瘤浸润性淋巴细胞细胞或来自淋巴细胞或单核细胞浓缩物或来自组织细胞制剂的活化的杀伤单核细胞。
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2.发明授权公开(公告)号：US06596179B2
公开(公告)日：2003-07-22
申请号：US09785949
申请日：2001-02-16
IPC分类号： B01D3315
CPC分类号： G01N33/56972 , A61M1/3618 , A61M1/362 , A61M1/3696 , C12Q1/24 , G01N33/5002 , G01N33/5005 , G01N33/569 , Y10T436/111666 , Y10T436/25375 , Y10T436/2575
摘要： Apparatus for withdrawing whole blood from a donor, separating the whole blood to obtain a target cell population and returning a selected one of either the target cell population or at least a portion of the whole blood remainder to the donor or patient. A disposable separation inset for use in association with a rotatable separation device of the apparatus may also be provided. The disposable inset may include a chamber for receiving a cell suspension that includes the target cell, a receptacle for containing particles having a binding site for selectively binding to the target cell and a means for introducing the particles into the chamber.
摘要翻译： 用于从供体抽出全血的装置，分离全血以获得靶细胞群，并将目标细胞群体中的所选择的一个或全部血液剩余物的至少一部分返回给供体或患者。 还可以提供与该装置的可旋转分离装置相关联使用的一次性分离插入物。 一次性插图可以包括用于接收包括靶细胞的细胞悬浮液的腔室，用于容纳具有用于选择性地结合靶细胞的结合位点的颗粒的容器和用于将颗粒引入腔室的装置。
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3.发明授权Apparatus for separation of biologic components from heterogeneous cell populations 失效用于分离生物成分与异种细胞群体的装置公开(公告)号：US06221315B1
公开(公告)日：2001-04-24
申请号：US09290653
申请日：1999-04-12
IPC分类号： B01L1100
CPC分类号： G01N33/56972 , A61M1/3618 , A61M1/362 , A61M1/3696 , C12Q1/24 , G01N33/5002 , G01N33/5005 , G01N33/569 , Y10T436/111666 , Y10T436/25375 , Y10T436/2575
摘要： Apparatus for separating a biological component from a biological fluid are disclosed. The apparatus includes a chamber for receiving a biological fluid including a biological component, and a rotor for creating a centrifugal field within the chamber. A source of particles may be included wherein the particles have a binding site for selectively binding to the biological component. The particles may be aseptically introduced into the chamber. The apparatus may withdraw whole blood from a donor, separate the whole blood to obtain a target cell population and return a selected one of either the target cell population or at least a portion of the whole blood remainder to the donor or patient. A disposable separation inset for use in conjunction with a rotatable separation device such as the apparatus described above may also be provided.
摘要翻译： 公开了用于从生物流体分离生物组分的装置。 该装置包括用于接收包括生物组分的生物流体的腔室和用于在腔室内产生离心场的转子。 可以包括颗粒源，其中颗粒具有用于选择性结合生物组分的结合位点。 颗粒可以无菌地引入室中。 该装置可以从供体抽出全血，分离全血以获得靶细胞群并将目标细胞群体中的所选择的一个或全部血液剩余物的至少一部分返回给供体或患者。 还可以提供与诸如上述装置的可旋转分离装置结合使用的一次性分离插入件。
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4.发明授权
公开(公告)号：US5641622A
公开(公告)日：1997-06-24
申请号：US255107
申请日：1994-06-07
申请人： William C. Lake , Richard Giesler , Dennis Van Epps , John R. Chapman , Jeffrey A. Martinson , Dale R. Ellis , Frederick Aono , Daniel F. Bischof
发明人： William C. Lake , Richard Giesler , Dennis Van Epps , John R. Chapman , Jeffrey A. Martinson , Dale R. Ellis , Frederick Aono , Daniel F. Bischof
IPC分类号： A61M1/36 , C12N5/00 , C12N5/02 , C12N5/06 , C12Q1/24 , G01N33/50 , G01N33/569 , A01N1/02 , C12N1/02 , C12Q1/68
CPC分类号： G01N33/56972 , A61M1/362 , A61M1/3696 , C12Q1/24 , G01N33/5002 , G01N33/5005 , G01N33/569 , Y10T436/111666 , Y10T436/25375 , Y10T436/2575
摘要： A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.
摘要翻译： 提供了结合免疫亲和分离和连续流离心分离技术的方法，用于从异质细胞混合物中选择性分离成核的异质细胞群。 非均相细胞混合物紧密接触以促进与附着有活性结合特定所需类型的细胞的细胞混合物的颗粒结合。 选择颗粒使得颗粒/细胞共轭体的沉降速度与细胞混合物中的其它细胞的沉降速度充分不同，以允许其通过连续流动细胞分离器分离。 该方法以预期的免疫亲和力分离的高精度快速处理大量的细胞混合物，并且可用于分离例如来自全血，骨髓浓缩物或外周血干细胞浓缩物的各种类型的白细胞; 或淋巴因子活化的杀伤细胞的前体，肿瘤浸润性淋巴细胞细胞或来自淋巴细胞或单核细胞浓缩物或来自组织细胞制剂的活化的杀伤单核细胞。
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公开(公告)号：US20130045852A1
公开(公告)日：2013-02-21
申请号：US13199129
申请日：2011-08-19
申请人： John R. Chapman , Rodney Sparks
发明人： John R. Chapman , Rodney Sparks
CPC分类号： B01D21/26 , A61M1/3693 , B01D21/262 , B01D2221/10 , B01L3/5021 , B01L2200/026 , B01L2300/0864 , G01N33/491
摘要： The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation. A medium density fluid is used within the vessel to further isolate constituents of differing densities.
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公开(公告)号：US20120193274A1
公开(公告)日：2012-08-02
申请号：US13506145
申请日：2012-03-30
IPC分类号： B01D21/26
CPC分类号： B01D21/26 , A61M1/029 , A61M2202/0413 , A61M2202/0464 , A61M2202/10 , B01D21/262 , B01D21/307 , B01D21/34 , B01D2221/10 , B01L3/50215 , B01L2300/048 , B01L2300/049 , B01L2300/0681 , B01L2400/0605 , B01L2400/0611 , G01N2035/00495
摘要： A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.
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公开(公告)号：US20120122649A1
公开(公告)日：2012-05-17
申请号：US13374983
申请日：2012-01-26
IPC分类号： B01D43/00
CPC分类号： B01D21/26 , A61M1/029 , A61M2202/0413 , A61M2202/0464 , A61M2202/10 , B01D21/262 , B01D21/307 , B01D21/34 , B01D2221/10 , B01L3/50215 , B01L2300/048 , B01L2300/049 , B01L2300/0681 , B01L2400/0605 , B01L2400/0611 , G01N2035/00495
摘要： A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.
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8.发明授权Mass spectrometer system including an ion source operable under high pressure conditions, and a two-stage pumping arrangement 失效包括在高压条件下可操作的离子源的质谱仪系统和两级泵送装置
公开(公告)号：US5164593A
公开(公告)日：1992-11-17
申请号：US662468
申请日：1991-02-28
摘要： A mass spectrometer system includes an ion source effective to produce ions within a viscous flow region. A pumping arrangement is effective to produce a further viscous flow region of lower pressure than the first region, ions passing through the further region into a molecular flow region. The energy of the ions is defined as they enter the molecular flow region.
摘要翻译： 质谱仪系统包括有效地在粘性流动区域内产生离子的离子源。 泵送装置有效地产生比第一区域更低的压力的另外的粘性流动区域，离子通过另外的区域进入分子流动区域。 离子的能量定义为它们进入分子流动区域。
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公开(公告)号：US20120213754A1
公开(公告)日：2012-08-23
申请号：US13398739
申请日：2012-02-16
申请人： John R. Chapman
发明人： John R. Chapman
CPC分类号： C12N5/0653 , A61J1/10 , A61K35/12 , A61K35/14 , A61K35/15 , A61K35/16 , A61K35/18 , A61K35/19 , B65D81/266 , C12N5/0647 , C12N5/0663 , C12N5/0665 , C12N5/0667 , C12N2500/02
摘要： An apparatus for the preparation of cells for implantation into a living body is disclosed. The apparatus comprises a vessel substantially impermeable to gaseous oxygen; and fluid within said vessel, the fluid having a maximal dissolved oxygen capacity substantially equivalent to normal saline yet having a hypoxic oxygen concentration between about 0% to about 5% of said maximal dissolved oxygen capacity. The bag oxygen concentration level remains low when the apparatus is stored at 22°-25° C. in normal atmospheric conditions for a period of at least 30 days. The present invention simplifies the process of achieving donor cell hypoxic preconditioning for cell implantation, and may be used to bathe said cells to be transplanted for a sufficient time to activate the hypoxic metabolic pathway.
摘要翻译： 公开了一种用于制备用于植入生物体的细胞的装置。 该装置包括基本上不透气态的容器; 和所述容器内的流体，所述流体具有基本上等于生理盐水的最大溶解氧能力，但是具有在所述最大溶解氧容量的约0％至约5％之间的缺氧氧浓度。 当装置在正常大气条件下在22°-25℃下储存至少30天时，袋氧浓度水平保持较低。 本发明简化了用于细胞植入的供体细胞缺氧预处理的过程，并且可以用于洗涤待移植的细胞足够的时间以激活缺氧代谢途径。
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10.发明申请Apparatus and method for separating and isolating components of a biological fluid 有权用于分离和分离生物流体组分的装置和方法公开(公告)号：US20120128552A1
公开(公告)日：2012-05-24
申请号：US13374988
申请日：2012-01-24
IPC分类号： B04B1/00
CPC分类号： B01D21/26 , A61M1/029 , A61M2202/0413 , A61M2202/0464 , A61M2202/10 , B01D21/262 , B01D21/307 , B01D21/34 , B01D2221/10 , B01L3/50215 , B01L2300/048 , B01L2300/049 , B01L2300/0681 , B01L2400/0605 , B01L2400/0611 , G01N2035/00495
摘要： A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.
摘要翻译： 一种用于分离和分离生物流体组分的装置，包括用于容纳待处理流体的容器，用于在提供与其进行填充和抽出连通的同时关闭容器的管帽组件;设置在容器内用于漏斗和控制生物 流体流入浮子内的反向圆顶形隔离室，并控制生物流体流出隔离室，用于在离心过程中进行隔离室内至少一种组分或分离的生物流体流的密封隔离 处理。 所述装置还包括用于连接设置在所述浮子组件内的抽吸通道的柔性管和所述管帽组件的抽吸阀，用于允许至少提取在所述室内封装或隔离的所述一个部件或部件。
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