公开(公告)号：US4752583A

公开(公告)日：1988-06-21

申请号：US676162

申请日：1984-11-29

申请人： Ronald H. Jensen ,  Martin Vanderlaan ,  William L. Bigbee ,  Larry H. Stanker ,  Elbert W. Branscomb ,  Robert J. Grabske

发明人： Ronald H. Jensen ,  Martin Vanderlaan ,  William L. Bigbee ,  Larry H. Stanker ,  Elbert W. Branscomb ,  Robert J. Grabske

IPC分类号： G01N33/72 ,  C12N5/00 ,  C07K15/04

CPC分类号： G01N33/721 ,  Y10S530/861

摘要： The present invention provides monoclonal antibodies specific to and distinguish between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype.

摘要翻译： 本发明提供对血红蛋白S和血红蛋白A特异性和区别的单克隆抗体及其生产和使用方法。 这些抗体能够通过仅一个氨基酸残基来区分彼此不同的两种血红蛋白类型。 根据本方法制备的抗体可用作免疫荧光标记物，以列举由于干细胞中的体细胞突变而导致血红蛋白基因表达改变的性质的循环红细胞。 这样的测量被认为是人体内体内细胞体细胞突变的测定。 由于根据本发明产生的单克隆抗体对血红蛋白S表现出高度的特异性和更高的亲和力，所以它们适用于标记人红细胞用于血红蛋白基因型的流式细胞检测。

公开(公告)号：US5334528A

公开(公告)日：1994-08-02

申请号：US428537

申请日：1989-10-30

申请人： Larry H. Stanker ,  Martin Vanderlaan ,  Bruce E. Watkins

发明人： Larry H. Stanker ,  Martin Vanderlaan ,  Bruce E. Watkins

IPC分类号： C07K16/44 ,  C12N5/20 ,  C07K15/00

CPC分类号： C07K16/44 ,  Y10S436/815

摘要： Methods are described for making specific monoclonal antibodies useful for detection of cyclodienes in foods and environmental samples. Monoclonal antibodies specifically reactive with cyclodienes can detect accumulated pesticides in food, tissue or environmental samples. Extraction and preparation of organic samples for immunoassay in a polar-nonpolar reaction medium permits detection of halogenated organic ring structures at concentrations in samples.

摘要翻译： 描述了用于制备特异性单克隆抗体用于检测食品和环境样品中的环糊精的方法。 与环二烯特异性反应的单克隆抗体可以检测食品，组织或环境样品中积累的农药。 在极性 - 非极性反应介质中提取和制备用于免疫测定的有机样品允许在样品浓度下检测卤代有机环结构。

公开(公告)号：US5108900A

公开(公告)日：1992-04-28

申请号：US319102

申请日：1989-03-06

申请人： Larry H. Stanker ,  Martin Vanderlaan ,  Bruce E. Watkins ,  Jeanette M. Van Emon ,  Carolyn L. Bigbee

发明人： Larry H. Stanker ,  Martin Vanderlaan ,  Bruce E. Watkins ,  Jeanette M. Van Emon ,  Carolyn L. Bigbee

IPC分类号： A61K39/395 ,  C07K14/435 ,  C07K16/00 ,  C07K16/16 ,  C07K16/44 ,  C07K17/02 ,  C12N5/10 ,  C12N5/20 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/53 ,  G01N33/577

CPC分类号： C07K16/16 ,  Y10S436/815 ,  Y10S436/822

摘要： Methods are described for making specific monoclonal antibodies which may be used in a sensitive immunoassay for detection of synthetic pyrethroids in foods and environmental samples. Appropriate sample preparation and enzyme amplification of the immunoassay for this widely-used class of pesticides permits detection at low levels in laboratory and field tested samples.

摘要翻译： 描述了用于制备特异性单克隆抗体的方法，其可用于敏感免疫测定以检测食品和环境样品中的合成拟除虫菊酯。 这种广泛使用的农药类型的适当的样品制备和酶扩增免疫测定允许在实验室和现场测试样品中检测到低水平。

公开(公告)号：US5053336A

公开(公告)日：1991-10-01

申请号：US380638

申请日：1989-07-17

申请人： Martin Vanderlaan ,  Bruce E. Watkins ,  Larry H. Stanker

发明人： Martin Vanderlaan ,  Bruce E. Watkins ,  Larry H. Stanker

IPC分类号： C07K16/44

CPC分类号： C07K16/44 ,  Y10S530/809

摘要： Monoclonal antibodies are described which have specific affinities for halogenated nucleoside analogs and are preferentially selective for one particular halogen. Such antibodies, when incorporated into immunochemical reagents, may be used to identify and independently quantify the cell division character of more than one population or subpopulation in flow cytometric measurements. Independent assessment of division activity in cell sub-populations facilitates selection of appropriate time and dose for administration of anti-proliferative agents. The hybridomas which secrete halogen selective antibodies and the method of making them are described.

摘要翻译： 描述了对卤代核苷类似物具有特异性亲和力的单克隆抗体，并且对于一种特定的卤素优选是选择性的。 当掺入免疫化学试剂时，这样的抗体可用于鉴定和独立地定量流式细胞术测量中多于一个群体或亚群体的细胞分裂特征。 独立评估细胞亚种群中的分裂活性有助于选择合适的施用抗增殖剂的时间和剂量。 描述了分泌卤素选择性抗体的杂交瘤及其制备方法。

公开(公告)号：US4798807A

公开(公告)日：1989-01-17

申请号：US877909

申请日：1986-06-24

申请人： Martin Vanderlaan ,  Larry H. Stanker ,  Bruce E. Watkins ,  Nina R. Bailey

发明人： Martin Vanderlaan ,  Larry H. Stanker ,  Bruce E. Watkins ,  Nina R. Bailey

IPC分类号： G01N33/53 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/44 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC分类号： C07K16/44 ,  Y10S435/948 ,  Y10S436/815 ,  Y10S530/808

摘要： Compositions of matter are described which include five monoclonal antibodies that react with dioxins and dibenzofurans, and the five hybridomas that produce these monoclonal antibodies. In addition, a method for the use of these antibodies in a sensitive immunoassay for dioxins and dibenzofurans is given, which permits detection of these pollutants in samples at concentrations in the range of a few parts per billion.

摘要翻译： 描述了包括与二恶英和二苯并呋喃反应的五种单克隆抗体以及产生这些单克隆抗体的五种杂交瘤的物质组合物。 此外，给出了在二恶英和二苯并呋喃的敏感免疫测定中使用这些抗体的方法，其允许以浓度在几十分之几的浓度检测样品中的这些污染物。

公开(公告)号：US5429925A

公开(公告)日：1995-07-04

申请号：US966565

申请日：1992-10-26

申请人： Martin Vanderlaan ,  Larry H. Stanker ,  Bruce E. Watkins ,  Peter Petrovic ,  Siegbert Gorbach

发明人： Martin Vanderlaan ,  Larry H. Stanker ,  Bruce E. Watkins ,  Peter Petrovic ,  Siegbert Gorbach

IPC分类号： G01N33/53 ,  G01N33/535 ,  G01N33/537 ,  G01N33/543

CPC分类号： G01N33/5308 ,  Y10S435/803 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S436/815 ,  Y10S436/825

摘要： A method is described for the use of monoclonal antibodies in a sensitive immunoassay for halogenated dioxins and dibenzofurans in industrial samples which contain impurities. Appropriate sample preparation and selective enzyme amplification of the immunoassay sensitivity permits detection of dioxin contaminants in industrial or environmental samples at concentrations in the range of a few parts per trillion.

摘要翻译： 描述了在含有杂质的工业样品中的用于卤化二恶英和二苯并呋喃的敏感免疫测定中使用单克隆抗体的方法。 适当的样品制备和选择性酶扩增免疫测定灵敏度允许检测工业或环境样品中二恶英污染物，浓度范围在几万分之几。

公开(公告)号：US5908781A

公开(公告)日：1999-06-01

申请号：US766250

申请日：1996-12-10

申请人： Larry H. Stanker ,  Beate G. Rose ,  John R. DeLoach ,  Carol Kamps Holtzapple

发明人： Larry H. Stanker ,  Beate G. Rose ,  John R. DeLoach ,  Carol Kamps Holtzapple

IPC分类号： C07K16/14 ,  C12P21/08 ,  G01N33/53 ,  C12N5/12

CPC分类号： C07K16/14 ,  G01N33/5308 ,  Y10S435/81

摘要： Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to the ceftiofur. These hybridomas may be obtained by using as an immunization agent or immunogen, desfuroyl ceftiofur which has been conjugated to an immunogenic carrier. Ceftiofur in biological samples may be detected and quantified by contacting the sample with the antibodies to form a ceftiofur/antibody immunocomplex when ceftiofur is present, which immunocomplex may then be detected. The monoclonal antibodies may also be incorporated into kits for the detection and quantification of ceftiofur.

摘要翻译： 已经产生产生和分泌选择性结合头孢噻呋的单克隆抗体的杂交瘤细胞系。 这些杂交瘤可以通过使用已经与免疫原性载体缀合的免疫剂或免疫原，脱氧呋喃甲基头孢噻呋来获得。 生物样品中的头孢噻呋可以通过使样品与抗体接触以形成头孢噻呋/抗体免疫复合物，当头孢噻呋存在时可以检测和定量，然后可以检测该免疫复合物。 单克隆抗体也可以并入用于检测和定量头孢噻呋的试剂盒中。

公开(公告)号：US20120009209A1

公开(公告)日：2012-01-12

申请号：US13157216

申请日：2011-06-09

申请人： Robert M. Hnasko ,  Larry H. Stanker ,  Stanley B. Prusiner

发明人： Robert M. Hnasko ,  Larry H. Stanker ,  Stanley B. Prusiner

IPC分类号： A61K39/00 ,  A61P31/00 ,  C07K16/18 ,  G01N33/566 ,  G01N21/76

CPC分类号： C07K16/44 ,  C07K16/2872 ,  C07K2317/33 ,  C07K2317/34 ,  G01N33/68 ,  G01N33/6896 ,  G01N2500/02 ,  G01N2800/2828

摘要： Peptide sequences that specifically bind infectious prion protein for the generation of antibodies and therapeutic agents are disclosed herein.

摘要翻译： 本文公开了特异性结合感染性朊病毒蛋白以产生抗体和治疗剂的肽序列。

公开(公告)号：US5691200A

公开(公告)日：1997-11-25

申请号：US675749

申请日：1996-07-03

申请人： Michael H. Kogut ,  John R. DeLoach ,  Larry H. Stanker ,  Rita B. Moyes ,  Billy M. Hargis

发明人： Michael H. Kogut ,  John R. DeLoach ,  Larry H. Stanker ,  Rita B. Moyes ,  Billy M. Hargis

IPC分类号： A61K38/00 ,  C07K14/52 ,  C07K14/535 ,  C12N5/02 ,  C12N5/06

CPC分类号： C07K14/535 ,  C07K14/52 ,  A61K38/00

摘要： Immortal avian T lymphocyte cell lines which produce and secrete immune lymphokines are disclosed. These cell lines may be produced from T cells recovered from fowl which have been hyperimmunized in vivo. The activated T cells are first exposed in vitro to a mitogen effective for secondary stimulation thereof, and then virally transformed to produce an immortal cell line. When cultured in vitro, the cell lines produce and secrete immune lymphokines which may be administered to fowl to increase their resistance to infections.

摘要翻译： 公开了产生和分泌免疫淋巴因子的不朽禽类T淋巴细胞系。 这些细胞系可以从在体内被超免疫的家禽中回收的T细胞产生。 首先将活化的T细胞在体外暴露于对其二次刺激有效的促分裂原，然后进行病毒转化以产生永生细胞系。 当在体外培养时，细胞系产生和分泌可以施用于家禽以增加其对感染的抗性的免疫淋巴因子。

公开(公告)号：US5209919A

公开(公告)日：1993-05-11

申请号：US693248

申请日：1991-04-26

申请人： Kenneth W. Turteltaub ,  John S. Vogel ,  James S. Felton ,  Barton L. Gledhill ,  Jay C. Davis ,  Larry H. Stanker

发明人： Kenneth W. Turteltaub ,  John S. Vogel ,  James S. Felton ,  Barton L. Gledhill ,  Jay C. Davis ,  Larry H. Stanker

IPC分类号： A61K51/04 ,  G01N33/60

CPC分类号： G01N33/60 ,  A61K51/0423 ,  A61K51/0459 ,  A61K2123/00 ,  Y10T436/13

摘要： Disclosed is a method of quantifying molecules in biological substances, comprising:a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere,b. preparing a long-lived radioisotope labeled reactive chemical specie,c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof,d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host,e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources,f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and,g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

摘要翻译： 公开了一种量化生物物质中的分子的方法，包括：a。 选择其中存在放射性同位素的生物宿主的浓度等于或小于环境生物圈中的浓度，b。 制备长寿命放射性同位素标记的反应性化学物质，c。 以足够低的剂量将所述化学物质施用于所述生物宿主，以避免对其生物系统的显着明显的损害，d。 允许一段时间足以在所述宿主的所有生物系统中传播所述化学物质与所述宿主的相互作用，例如， 从足够的方式从所述宿主中分离出生物物质的反应级分，以避免所述物质从外界来源污染，f。 通过合适的方式将生物物质的所述部分转化为在几种可能的离子源中的至少一种中有效地产生带电离子的材料，而不引入显着的同位素分馏，以及g。 通过直接同位素计数测量所述材料中的放射性同位素浓度。