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    Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof
    1.
    发明授权
    Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof 失效
    人血红蛋白S的单克隆抗体及其制备的细胞系

    公开(公告)号：US4752583A

    公开(公告)日：1988-06-21

    申请号：US676162

    申请日：1984-11-29

    申请人： Ronald H. Jensen ,  Martin Vanderlaan ,  William L. Bigbee ,  Larry H. Stanker ,  Elbert W. Branscomb ,  Robert J. Grabske

    发明人： Ronald H. Jensen ,  Martin Vanderlaan ,  William L. Bigbee ,  Larry H. Stanker ,  Elbert W. Branscomb ,  Robert J. Grabske

    IPC分类号： G01N33/72 ,  C12N5/00 ,  C07K15/04

    CPC分类号： G01N33/721 ,  Y10S530/861

    摘要： The present invention provides monoclonal antibodies specific to and distinguish between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype.

    摘要翻译： 本发明提供对血红蛋白S和血红蛋白A特异性和区别的单克隆抗体及其生产和使用方法。 这些抗体能够通过仅一个氨基酸残基来区分彼此不同的两种血红蛋白类型。 根据本方法制备的抗体可用作免疫荧光标记物，以列举由于干细胞中的体细胞突变而导致血红蛋白基因表达改变的性质的循环红细胞。 这样的测量被认为是人体内体内细胞体细胞突变的测定。 由于根据本发明产生的单克隆抗体对血红蛋白S表现出高度的特异性和更高的亲和力，所以它们适用于标记人红细胞用于血红蛋白基因型的流式细胞检测。

    Monoclonal antibodies to cyclodiene insecticides and method for detecting the same
    2.
    发明授权
    Monoclonal antibodies to cyclodiene insecticides and method for detecting the same 失效
    环二烯杀虫剂的单克隆抗体及其检测方法

    公开(公告)号：US5334528A

    公开(公告)日：1994-08-02

    申请号：US428537

    申请日：1989-10-30

    申请人： Larry H. Stanker ,  Martin Vanderlaan ,  Bruce E. Watkins

    发明人： Larry H. Stanker ,  Martin Vanderlaan ,  Bruce E. Watkins

    IPC分类号： C07K16/44 ,  C12N5/20 ,  C07K15/00

    CPC分类号： C07K16/44 ,  Y10S436/815

    摘要： Methods are described for making specific monoclonal antibodies useful for detection of cyclodienes in foods and environmental samples. Monoclonal antibodies specifically reactive with cyclodienes can detect accumulated pesticides in food, tissue or environmental samples. Extraction and preparation of organic samples for immunoassay in a polar-nonpolar reaction medium permits detection of halogenated organic ring structures at concentrations in samples.

    摘要翻译： 描述了用于制备特异性单克隆抗体用于检测食品和环境样品中的环糊精的方法。 与环二烯特异性反应的单克隆抗体可以检测食品，组织或环境样品中积累的农药。 在极性 - 非极性反应介质中提取和制备用于免疫测定的有机样品允许在样品浓度下检测卤代有机环结构。

    Monoclonal antibodies for the separate detection of halodeoxyuridines and method for their use
    4.
    发明授权
    Monoclonal antibodies for the separate detection of halodeoxyuridines and method for their use 失效
    用于单独检测卤代脱氧尿苷的单克隆抗体及其使用方法

    公开(公告)号：US5053336A

    公开(公告)日：1991-10-01

    申请号：US380638

    申请日：1989-07-17

    申请人： Martin Vanderlaan ,  Bruce E. Watkins ,  Larry H. Stanker

    发明人： Martin Vanderlaan ,  Bruce E. Watkins ,  Larry H. Stanker

    IPC分类号： C07K16/44

    CPC分类号： C07K16/44 ,  Y10S530/809

    摘要： Monoclonal antibodies are described which have specific affinities for halogenated nucleoside analogs and are preferentially selective for one particular halogen. Such antibodies, when incorporated into immunochemical reagents, may be used to identify and independently quantify the cell division character of more than one population or subpopulation in flow cytometric measurements. Independent assessment of division activity in cell sub-populations facilitates selection of appropriate time and dose for administration of anti-proliferative agents. The hybridomas which secrete halogen selective antibodies and the method of making them are described.

    摘要翻译： 描述了对卤代核苷类似物具有特异性亲和力的单克隆抗体，并且对于一种特定的卤素优选是选择性的。 当掺入免疫化学试剂时，这样的抗体可用于鉴定和独立地定量流式细胞术测量中多于一个群体或亚群体的细胞分裂特征。 独立评估细胞亚种群中的分裂活性有助于选择合适的施用抗增殖剂的时间和剂量。 描述了分泌卤素选择性抗体的杂交瘤及其制备方法。

    Monoclonal antibodies to ceftiofur
    7.
    发明授权
    Monoclonal antibodies to ceftiofur 失效
    头孢噻呋的单克隆抗体

    公开(公告)号：US5908781A

    公开(公告)日：1999-06-01

    申请号：US766250

    申请日：1996-12-10

    申请人： Larry H. Stanker ,  Beate G. Rose ,  John R. DeLoach ,  Carol Kamps Holtzapple

    发明人： Larry H. Stanker ,  Beate G. Rose ,  John R. DeLoach ,  Carol Kamps Holtzapple

    IPC分类号： C07K16/14 ,  C12P21/08 ,  G01N33/53 ,  C12N5/12

    CPC分类号： C07K16/14 ,  G01N33/5308 ,  Y10S435/81

    摘要： Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to the ceftiofur. These hybridomas may be obtained by using as an immunization agent or immunogen, desfuroyl ceftiofur which has been conjugated to an immunogenic carrier. Ceftiofur in biological samples may be detected and quantified by contacting the sample with the antibodies to form a ceftiofur/antibody immunocomplex when ceftiofur is present, which immunocomplex may then be detected. The monoclonal antibodies may also be incorporated into kits for the detection and quantification of ceftiofur.

    摘要翻译： 已经产生产生和分泌选择性结合头孢噻呋的单克隆抗体的杂交瘤细胞系。 这些杂交瘤可以通过使用已经与免疫原性载体缀合的免疫剂或免疫原，脱氧呋喃甲基头孢噻呋来获得。 生物样品中的头孢噻呋可以通过使样品与抗体接触以形成头孢噻呋/抗体免疫复合物，当头孢噻呋存在时可以检测和定量，然后可以检测该免疫复合物。 单克隆抗体也可以并入用于检测和定量头孢噻呋的试剂盒中。

    Method of measurement in biological systems
    10.
    发明授权
    Method of measurement in biological systems 失效
    生物系统中的测量方法

    公开(公告)号：US5209919A

    公开(公告)日：1993-05-11

    申请号：US693248

    申请日：1991-04-26

    申请人： Kenneth W. Turteltaub ,  John S. Vogel ,  James S. Felton ,  Barton L. Gledhill ,  Jay C. Davis ,  Larry H. Stanker

    发明人： Kenneth W. Turteltaub ,  John S. Vogel ,  James S. Felton ,  Barton L. Gledhill ,  Jay C. Davis ,  Larry H. Stanker

    IPC分类号： A61K51/04 ,  G01N33/60

    CPC分类号： G01N33/60 ,  A61K51/0423 ,  A61K51/0459 ,  A61K2123/00 ,  Y10T436/13

    摘要： Disclosed is a method of quantifying molecules in biological substances, comprising:a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere,b. preparing a long-lived radioisotope labeled reactive chemical specie,c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof,d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host,e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources,f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and,g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

    摘要翻译： 公开了一种量化生物物质中的分子的方法，包括：a。 选择其中存在放射性同位素的生物宿主的浓度等于或小于环境生物圈中的浓度，b。 制备长寿命放射性同位素标记的反应性化学物质，c。 以足够低的剂量将所述化学物质施用于所述生物宿主，以避免对其生物系统的显着明显的损害，d。 允许一段时间足以在所述宿主的所有生物系统中传播所述化学物质与所述宿主的相互作用，例如， 从足够的方式从所述宿主中分离出生物物质的反应级分，以避免所述物质从外界来源污染，f。 通过合适的方式将生物物质的所述部分转化为在几种可能的离子源中的至少一种中有效地产生带电离子的材料，而不引入显着的同位素分馏，以及g。 通过直接同位素计数测量所述材料中的放射性同位素浓度。