公开(公告)号：US11097269B2

公开(公告)日：2021-08-24

申请号：US15945272

申请日：2018-04-04

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Keisuke Goto ,  Yoichi Makino ,  Akihiro Hoshino

IPC: B01L3/00 ,  G01N21/03 ,  G01N33/52 ,  B81B3/00 ,  B81B1/00 ,  G01N21/64 ,  B01L7/00

Abstract: A microfluidic device includes: a base plate allowing an electromagnetic wave to pass therethrough and having no autofluorescence; a microwell array formed on the base plate and including a wall layer in which a plurality of through-holes are formed in a thickness direction; and a lid member disposed opposite to the base plate in a state of being separated from the wall layer, wherein microwells are formed by the base plate and the through-holes formed in the wall layer, and wherein the wall layer is formed of a material containing a colored component that absorbs an electromagnetic wave of a predetermined wavelength.

公开(公告)号：US20180299380A1

公开(公告)日：2018-10-18

申请号：US16018277

申请日：2018-06-26

Applicant: Toppan Printing Co., Ltd.

Inventor： Yoichi Makino ,  Keisuke Goto

IPC: G01N21/64 ,  G01N21/05 ,  G01N35/08 ,  C12Q1/25 ,  C12Q1/68

Abstract: A microfluidic device includes a substrate having an electromagnetic wave transmission property, a lid member facing the substrate and being separated from the substrate such that a flow channel is formed between the substrate and the lid member, a light absorption layer which is placed in the flow channel and absorbs an electromagnetic wave, and a microwell array formed on the substrate and having plural microwells that are open to the flow channel to receive a target of analysis.

公开(公告)号：US20170233790A1

公开(公告)日：2017-08-17

申请号：US15586622

申请日：2017-05-04

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Yoichi Makino ,  Tomoyuki Ozawa ,  Shuichi Akashi ,  Tomoko kunitomi

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/682 ,  B01L3/50853 ,  B01L2300/04 ,  B01L2300/0829 ,  B01L2300/168 ,  C12M33/00 ,  C12M35/00 ,  C12Q1/68 ,  C12Q1/6825 ,  G01N33/53

Abstract: An array device including a base having wells, a cover positioned over the base with a gap from the base such that openings of the wells are covered by the cover with the gap in between, an injection port communicating with the gap, a discharge port communicating with the gap and positioned apart from the injection port, and a waste liquid vessel which collects liquid that flows from the gap via the discharge port and is positioned at a level different from the gap forming a channel. The discharge port is placed to discharge a surplus aqueous solution outside the wells from the discharge port by an oleaginous sealing liquid to be delivered from the injection port.

公开(公告)号：US11142731B2

公开(公告)日：2021-10-12

申请号：US15854355

申请日：2017-12-26

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Tomoko Kunitomi ,  Yoichi Makino

IPC: B01L3/00 ,  C12M1/34 ,  C12M1/00 ,  G01N21/64 ,  C12Q1/6825 ,  B01L7/00

Abstract: An analytical device includes a plate including a first substrate having a front surface and a back surface and a second substrate facing the back surface of the first substrate such that at least one detection space observable from outside is formed between the second substrate and the back surface of the first substrate, and an adapter. The first substrate has a first port having a through hole formed through the front surface and communicated with the detection space for delivering a liquid-containing substance into the detection space, the first substrate has a second port having a through hole formed through the front surface and communicated with the detection space for discharging a liquid-containing substance or a gas from the detection space, and the adapter is attachable to and detachable from the first port and the second port, and the front surface of the first substrate without the adapter is flat.

公开(公告)号：US10894973B2

公开(公告)日：2021-01-19

申请号：US15586622

申请日：2017-05-04

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Yoichi Makino ,  Tomoyuki Ozawa ,  Shuichi Akashi ,  Tomoko Kunitomi

IPC: C12Q1/68 ,  C12Q1/682 ,  G01N33/53 ,  C12M1/42 ,  C12M1/26 ,  B01L3/00 ,  C12Q1/6825

Abstract: An array device including a base having wells, a cover positioned over the base with a gap from the base such that openings of the wells are covered by the cover with the gap in between, an injection port communicating with the gap, a discharge port communicating with the gap and positioned apart from the injection port, and a waste liquid vessel which collects liquid that flows from the gap via the discharge port and is positioned at a level different from the gap forming a channel. The discharge port is placed to discharge a surplus aqueous solution outside the wells from the discharge port by an oleaginous sealing liquid to be delivered from the injection port.

公开(公告)号：US10718776B2

公开(公告)日：2020-07-21

申请号：US15914756

申请日：2018-03-07

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Yoichi Makino

IPC: G01N33/58 ,  C12Q1/68 ,  C12Q1/686 ,  G01N33/68

Abstract: A method of detecting a biological substance, including introducing a first substance dispersed in a solvent into a flow channel formed between a first substrate and a second substrate such that the first substance is placed in a first well formed in the first substrate, and detecting the first substance. The first well is formed such that the first well has an open side which faces downwards and is communicated with the flow channel, and the solvent has a specific gravity larger than a specific gravity of the first substance.

公开(公告)号：US12173354B2

公开(公告)日：2024-12-24

申请号：US16778618

申请日：2020-01-31

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Masayuki Ogino ,  Yoichi Makino

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6837 ,  C12Q1/6876

Abstract: A method of detecting a nucleic acid including mixing a fluid including a target nucleic acid with a detection reagent including a first enzyme for cleaving a first nucleic acid having a first flap and a second enzyme for cleaving a second nucleic acid such that the target nucleic acid, the first nucleic acid and the second nucleic acid form a complex as a first invasive structure, conducting a first reaction which causes the first enzyme to cleave the first flap of the first invasive structure and produces a third nucleic acid that forms a complex, as a second invasive structure, with a fourth nucleic acid having a second flap, and conducting a second reaction which causes the second enzyme to cleave the second flap of the second invasive structure and produces a cleaved product.

公开(公告)号：US11662313B2

公开(公告)日：2023-05-30

申请号：US16018277

申请日：2018-06-26

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Yoichi Makino ,  Keisuke Goto

IPC: G01N21/64 ,  C12Q1/68 ,  G01N33/487 ,  B01L3/00 ,  G01N21/05 ,  G01N35/08 ,  C12Q1/25

CPC classification number: G01N21/645 ,  B01L3/502715 ,  C12Q1/25 ,  C12Q1/68 ,  G01N21/05 ,  G01N21/6428 ,  G01N33/487 ,  G01N35/08 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/165 ,  G01N2021/058 ,  G01N2021/6482

Abstract: A microfluidic device includes a substrate having an electromagnetic wave transmission property, a lid member facing the substrate and being separated from the substrate such that a flow channel is formed between the substrate and the lid member, a light absorption layer which is placed in the flow channel and absorbs an electromagnetic wave, and a microwell array formed on the substrate and having plural microwells that are open to the flow channel to receive a target of analysis.

公开(公告)号：US11559804B2

公开(公告)日：2023-01-24

申请号：US16173102

申请日：2018-10-29

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Yoichi Makino ,  Akihiro Hoshino ,  Tomoko Kunitomi ,  Keisuke Goto ,  Yosuke Horiuchi

IPC: G01N21/64 ,  B01L3/00 ,  B01L7/00

Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.

公开(公告)号：US11414697B2

公开(公告)日：2022-08-16

申请号：US16171630

申请日：2018-10-26

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Masahiro Matsukawa ,  Yoichi Makino ,  Akihiro Hoshino

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/6844 ,  G01N21/64 ,  G01N33/68 ,  C12Q1/68 ,  G01N21/00 ,  C12M1/00 ,  G01N37/00 ,  C12N15/09 ,  G01N33/50 ,  C12N15/10

Abstract: A reaction container including a transparent base having a first surface having at least one region where recessed portions are formed and recessed from the first surface, and a cover member positioned such that the cover member forms a gap from the first surface inside the region and is welded to the transparent base outside the region. The cover member absorbs infrared light and transmits light having a wavelength within a range of visible light.