摘要:
The present invention relates to a polypeptide capable of increasing the production of L-methionine in a microorganism. In particular, the present invention relates to an YgaZ and YgaH polypeptide or a complex thereof, referred to herein as YgaZH polypeptide, which are novel putative L-methionine exporters, polynucleotides encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing L-methionine and/or S-adenosyl-methionine, comprising the steps of culturing the transformed microorganism to produce L-methionine and/or S-adenosyl-methionine, and isolating L-methionine and/or S-adenosyl-methionine. The transformed microorganism of the present invention produces L-methionine in a high yield, thereby being used for medicinal and pharmaceutical industries and feed industry, in particular, animal feeds
摘要:
A method of producing 1,2-propandiol is provided. The method includes incubating Klebsiella pneumoniae in a medium containing 10–30 g/L of a sugar carbon source excluding 6-deoxyhexose, in aerobic conditions; and separating 1,2-propandiol from the cultures. Using the method, 1,2-propandiol can be produced with a high yield by incubating Klebsiella pneumoniae in a medium containing a cheaper sugar carbon source.
摘要:
Provided are a polypeptide having glycerol-3-phosphate dehydrogenase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 1 and a polypeptide having glycerol-3-phosphate phosphatase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 2. Provided is also a method for producing glycerol, which includes: culturing a host cell transformed with a vector containing a polynucleotide including a first polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 1 and a second polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 2 which are operably linked to a suitable regulatory sequence; and recovering glycerol from the culture.
摘要翻译:提供了具有甘油-3-磷酸脱氢酶活性的多肽和与SEQ ID NO:1所示的氨基酸序列具有80%或更高同源性的多肽和具有甘油-3-磷酸磷酸酶活性的多肽和80%以上的多肽 提供了与序列号2所示的氨基酸序列的同源性。提供了一种生产甘油的方法,其包括:培养用含有多核苷酸转化的宿主细胞,所述载体含有编码氨基酸序列的第一多核苷酸, 编码SEQ ID NO:2所示的氨基酸序列的第二多核苷酸,其编码可操作地连接到合适的调节序列; 并从培养物中回收甘油。
摘要:
Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.
摘要:
Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.
摘要:
Disclosed herein are a method of preparing an L-methionine production strain by overexpressing proteins involved in L-methionine biosynthesis in an L-threonine production strain, a strain prepared by the method, and a method of producing L-methionine using the strain.
摘要:
Disclosed herein are a method of preparing an L-methionine production strain by overexpressing proteins involved in L-methionine biosynthesis in an L-threonine production strain, a strain prepared by the method, and a method of producing L-methionine using the strain.
摘要:
The present invention relates to an amino acid-producing microorganism capable of simultaneously utilizing glycerol as a carbon source, a method for preparing the microorganism, and a method for producing amino acids using the microorganism. According to the present invention, amino acids can be efficiently produced using a byproduct of biodiesel production, glycerol, thereby substituting a cheaper material for the conventional fermentation materials such as glucose.
摘要:
Provided are a microorganism capable of producing L-threonine and having an inactivated tyrR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.
摘要:
Provided are a microorganism capable of producing L-threonine and having an inactivated galR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.