公开(公告)号：US20230242871A1

公开(公告)日：2023-08-03

申请号：US18000072

申请日：2021-05-28

申请人： The Board of Trustees of the Leland Stanfor Junior University

发明人： Vittorio Sebastiano ,  Kyle M. Loh ,  Gugene Kang

IPC分类号： C12N5/0735

CPC分类号： C12N5/0611 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2506/45

摘要： Provided herein are, inter alia, compositions and methods for generating a population of PGCs with improved efficiencies and line-to-line consistency. Inductive signals are temporally dynamic: WNT activation for 12 hours incipiently differentiates primed hPSCs into posterior epiblast, while subsequently, sharp WNT inhibition together with BMP activation specifies PGCs. hPSC-derived PGCs can be easily purified by virtue of their CXCR4+PDGFRα-GARP-surface-marker profile.

公开(公告)号：US20190093078A1

公开(公告)日：2019-03-28

申请号：US16120342

申请日：2018-09-03

申请人： Technion Research & Development Foundation Limited

发明人： Michal AMIT ,  Joseph ITSKOVITZ-ELDOR

IPC分类号： C12N5/074

CPC分类号： C12N5/0607 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0606 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

公开(公告)号：US20180200305A1

公开(公告)日：2018-07-19

申请号：US15917370

申请日：2018-03-09

申请人： The General Hospital Corporation

发明人： Jonathan Lee Tilly ,  Joshua Johnson

IPC分类号： A61K35/54 ,  C12N5/074 ,  C12N15/877 ,  C12N5/0735 ,  A01K67/027

CPC分类号： A61K35/54 ,  A01K67/0271 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0393 ,  C12N5/0607 ,  C12N5/0611 ,  C12N15/8775

摘要： The present invention relates to female germline stem cells and their progenitors, methods of isolation thereof, and methods of use thereof.

公开(公告)号：US20170152476A1

公开(公告)日：2017-06-01

申请号：US15370969

申请日：2016-12-06

申请人： Shoukhrat Mitalipov ,  Masahito Tachibana

发明人： Shoukhrat Mitalipov ,  Masahito Tachibana

IPC分类号： C12N5/0735 ,  C12N15/873

CPC分类号： C12N5/0606 ,  C12N5/0603 ,  C12N5/0609 ,  C12N5/0611 ,  C12N5/16 ,  C12N15/873 ,  C12N15/8776 ,  C12N2500/44 ,  C12N2500/70 ,  C12N2500/84 ,  C12N2502/02 ,  C12N2506/02 ,  C12N2517/04

摘要： Human pluripotent embryonic stem cells produced by somatic cell nuclear transfer as well as methods of making and using said human pluripotent embryonic stem cells are disclosed.

公开(公告)号：US09546383B2

公开(公告)日：2017-01-17

申请号：US14276810

申请日：2014-05-13

申请人： Shoukhrat Mitalipov ,  Masahito Tachibana

发明人： Shoukhrat Mitalipov ,  Masahito Tachibana

IPC分类号： C12N5/00 ,  C12N15/873 ,  C12N5/16 ,  C12N5/073 ,  C12N5/075 ,  C12N5/0735 ,  C12N15/877

CPC分类号： C12N5/0606 ,  C12N5/0603 ,  C12N5/0609 ,  C12N5/0611 ,  C12N5/16 ,  C12N15/873 ,  C12N15/8776 ,  C12N2500/44 ,  C12N2500/70 ,  C12N2500/84 ,  C12N2502/02 ,  C12N2506/02 ,  C12N2517/04

摘要： Human pluripotent embryonic stem cells produced by somatic cell nuclear transfer as well as methods of making and using said human pluripotent embryonic stem cells are disclosed.

摘要翻译： 公开了通过体细胞核转移产生的人多能胚胎干细胞以及制备和使用所述人多能胚胎干细胞的方法。

公开(公告)号：US20160362657A1

公开(公告)日：2016-12-15

申请号：US14990539

申请日：2016-01-07

申请人： The General Hospital Corporation

发明人： Jonathan Tilly ,  Joshua Johnson

IPC分类号： C12N5/0735 ,  A61B17/43 ,  A61K35/14 ,  C12N5/078 ,  C12N5/075

CPC分类号： C12N5/0611 ,  A61B17/43 ,  A61K35/14 ,  A61K35/28 ,  A61K2035/124 ,  C12N5/0609 ,  C12N5/0634 ,  C12N2506/11 ,  C12P21/06

摘要： The application describes the use of peripheral blood derived germline stem cells and their progenitors, methods of isolation thereof, and methods of use thereof.

摘要翻译： 本申请描述了外源血源生殖干细胞及其祖细胞的使用，其分离方法及其使用方法。

公开(公告)号：US09410121B2

公开(公告)日：2016-08-09

申请号：US14578443

申请日：2014-12-21

申请人： Technion Research & Development Foundation Limited

发明人： Michal Amit ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/02 ,  C12N5/074 ,  C12N5/0735 ,  C12N5/00 ,  C12N5/0797

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0607 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。

公开(公告)号：US08945925B2

公开(公告)日：2015-02-03

申请号：US14058347

申请日：2013-10-21

申请人： Technion Research & Development Foundation Limited

发明人： Michal Amit ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/02 ,  C12N5/074 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0607 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。

公开(公告)号：US20140315301A1

公开(公告)日：2014-10-23

申请号：US14259997

申请日：2014-04-23

申请人： Yeda Research and Development Co. Ltd.

发明人： Yaqub HANNA ,  Noa Novershtern ,  Yoach Rais

IPC分类号： C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0611 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/42 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/999

摘要： Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

摘要翻译： 提供了一种分离的人类天真多能干细胞（PSC），其中：（i）当初始PSC是女性PSC时，所述初始女性PSC具有X-非活性特异性转录物（XIST）基因的两个未甲基化等位基因; 和（ii）当所述初始PSC是雄性PSC时，所述初始雄性PSC具有所述XIST基因的未甲基化等位基因。 还提供了包含ERK1 / 2抑制剂，GSK3β抑制剂，p38抑制剂，JNK抑制剂，STAT3活化剂和至少一种选自以下的药剂的培养基：bFGF，TGFβ1，PKC抑制剂， ROCK抑制剂和NOTCH抑制剂; 或至少选自TGFR抑制剂，FGFR抑制剂，PKC抑制剂，ROCK抑制剂和NOTCH抑制剂的药剂。

公开(公告)号：US20130252328A1

公开(公告)日：2013-09-26

申请号：US13787009

申请日：2013-03-06

申请人： DAVINCI BIOSCIENCES LLC

发明人： Francisco Silva ,  Rafael Gonzalez

IPC分类号： C12N5/071

CPC分类号： C12N5/0608 ,  C12N5/0607 ,  C12N5/0611 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/13

摘要： Adult human gonadal stem cells that are capable of differentiating into cells of the mesodermal lineage and ectodermal lineage are described.

摘要翻译： 描述了能够分化成中胚层谱系和外胚层谱系细胞的成年人性腺细胞干细胞。