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    Regioselective hydroxylation of isophorone
    1.
    发明授权
    Regioselective hydroxylation of isophorone 有权

    公开(公告)号：US12077801B2

    公开(公告)日：2024-09-03

    申请号：US16500497

    申请日：2018-04-06

    申请人： DSM IP Assets B.V.

    发明人： Werner Bonrath ,  Martin Schürmann ,  Michele Tavanti ,  Fabio Parmeggiani ,  Nicholas Turner ,  Andrea Mattevi ,  Jose Ruben Casstellanos

    IPC分类号： C12P7/26 ,  C12N9/02

    CPC分类号： C12P7/26 ,  C12N9/0081 ,  C12Y114/15006

    摘要： The present invention relates to novel process for the production of ketoisophorone via biocatalytic conversion of isophorone, in particular a one-pot biocatalytic system for conversion of α-isophorone in a two-step oxidation process, with a first oxidation being catalyzed by a heme containing oxidoreductase such as a cytochrome P450 monooxygenase followed by another oxidation which can be either a chemical reaction or a biocatalytic reaction, in particular wherein the oxidation is catalyzed by an NAD(P) or NADP(H)-dependent oxidoreductase. The invention further provides polypeptides and nucleic acid sequences coding for cytochrome P450 monooxygenase with modified (higher) substrate selectivity, total turnover numbers and/or (re)activity compared to the wild-type enzyme. Ketoisophorone is useful as building block in the synthesis of vitamins and carotenoids.

    Process for oxidation of steroids and genetically engineered cells used therein
    8.
    发明申请
    Process for oxidation of steroids and genetically engineered cells used therein 审中-公开
    用于其中使用的类固醇和基因工程细胞的氧化过程

    公开(公告)号：US20030108982A1

    公开(公告)日：2003-06-12

    申请号：US10084814

    申请日：2002-02-26

    申请人： Aventis Pharma S.A.

    发明人： Herman Slijkhuis ,  Eric Bastiaan Smaal ,  Gerardus Cornelis Maria Selten

    IPC分类号： C12P033/08 ,  C12N001/21 ,  C12N015/74

    CPC分类号： C12Y106/02004 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0095 ,  C12P33/00 ,  C12Y101/01053 ,  C12Y114/15004 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y118/01002

    摘要： An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting a of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17null-hydroxy-progesterone; the conversion of 17null-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host.

    摘要翻译： 可在重组宿主中操作的表达盒，其包含编码蛋白质的异源DNA编码序列，所述蛋白质单独或与一种或多种另外的蛋白质协同作用来催化用于将胆固醇转化为氢化可的松的生物学途径中的氧化步骤 ，该步骤选自：胆固醇转化为孕烯雌酚; 孕烯醇酮转化为孕酮; 孕酮转化为17α-羟基孕酮; 将17α-羟孕酮转化为皮质醇; 将皮质醇转化为氢化可的松，以及相应的控制顺序在所述宿主中有效。

    Cholesterol disposal fusion enzymes
    9.
    发明授权
    Cholesterol disposal fusion enzymes 失效
    胆固醇处理融合酶

    公开(公告)号：US5939318A

    公开(公告)日：1999-08-17

    申请号：US564090

    申请日：1996-02-05

    申请人： Walter L. Miller ,  Jennifer A. Harikrishna ,  Stephen M. Black

    发明人： Walter L. Miller ,  Jennifer A. Harikrishna ,  Stephen M. Black

    IPC分类号： C12N1/15 ,  C12N1/21 ,  C12N9/02 ,  C12P33/00 ,  C12N5/10 ,  C12N15/62 ,  C12N15/79

    CPC分类号： C12N9/0081 ,  C12N9/0095 ,  C12P33/005 ,  C12Y114/15006 ,  C12Y118/01002 ,  C07K2319/00

    摘要： Fusion enzymes having multiple segments of different biological activity including one segment having P450scc activity and at least one segment having electron-transfer activity for transferring electrons to P450scc are described along with genetic constructs for production of such enzymes and methods for their use. Methods for their use include cholesterol degradation in vitro or in vivo as well as conversion of cholesterol to other useful steroidal products including pregnenolone.

    摘要翻译： PCT No.PCT / US94 / 06698 Sec。 371日期：1996年2月5日 102（e）日期1996年2月5日PCT提交1994年6月9日PCT公布。 出版物WO94 / 29434 PCT 1994年12月22日的日期描述了具有不同生物学活性的多个片段的融合酶，包括具有P450scc活性的一个片段和至少一个具有用于将电子转移至P450scc的电子转移活性的片段以及用于生产这些酶的遗传构建体及其方法 使用。 其使用方法包括体外或体内的胆固醇降解以及胆固醇转化为其他有用的甾体产物，包括孕烯醇酮。