公开(公告)号：US20180120203A1

公开(公告)日：2018-05-03

申请号：US15567288

申请日：2016-04-18

申请人： Rowan University

发明人： Vince Beachley

IPC分类号： G01N1/36

CPC分类号： G01N1/36 ,  A01N1/0263 ,  G01N2001/364 ,  G01N2001/366 ,  G01N2001/368

摘要： Tissue samples embedded in settable, sectionable media such as paraffin can be cut into sections. Embedding one or more sectionable fiducial indicia in the medium permits identification of the plane along which the medium has been cut. However, prior methods of inserting indicia into embedded tissue sample blocks are cumbersome and difficult to perform. Sectionable fiducial indicia can be embedded in a block of medium by releasibly fixing the indicia to the sidewall or base of a mold used to shape the tissue block during embedding of a tissue sample in the medium block.

公开(公告)号：US09594087B2

公开(公告)日：2017-03-14

申请号：US14493985

申请日：2014-09-23

申请人： CellPath Ltd.

发明人： Paul Webber ,  Philip Webber ,  Richard Titcombe

IPC分类号： A61B10/00 ,  G01N35/00 ,  G01N1/36

CPC分类号： G01N35/00732 ,  G01N1/36 ,  G01N2001/366 ,  G01N2035/00801 ,  G01N2035/00831

摘要： A histology processing cassette comprising a box comprising a compartment for holding a biological tissue sample, the box having a bottom face comprising at least in part a sample support surface and being transmissible to radiation, an open top face, and two side walls, a back wall and a front wall, the box having dimensions greater than a standard size histology processing cassette and comprising a recess in the front wall adapted to receive a standard size cassette which has a front wall comprising a unique identifier for the biological tissue sample such that upon insertion of the standard cassette in the recess the unique identifier on the front wall of the standard cassette is readable.

摘要翻译： 一种组织学处理盒，包括一个盒子，该盒子包括用于容纳生物组织样本的隔室，该盒子具有一个底面，该底面至少部分地包括一个样品支撑表面，并且可透射到辐射，一个敞开的顶面和两个侧壁， 壁和前壁，该盒具有大于标准尺寸的组织学处理盒的尺寸，并且包括在前壁中的适于接收标准尺寸盒的凹部，其具有包括用于生物组织样本的唯一标识符的前壁， 将标准盒插入凹槽中，标识盒前壁上的唯一标识符是可读的。

公开(公告)号：US20150276574A1

公开(公告)日：2015-10-01

申请号：US14661020

申请日：2015-03-18

申请人： SYSMEX CORPORATION

发明人： Maria KIRIYAMA ,  Takuya KUBO

IPC分类号： G01N15/14 ,  G01N1/30

CPC分类号： G01N15/14 ,  G01N1/30 ,  G01N1/36 ,  G01N1/4044 ,  G01N1/44 ,  G01N15/1459 ,  G01N33/5306 ,  G01N2001/366 ,  G01N2015/1006

摘要： A cell analysis method is provided that comprises (a) removing embedding medium from a sample obtained from the tissue specimen which is aldehyde-fixed and embedded in the water-insoluble embedding medium, thereby obtaining the sample comprising the tissue from which the embedding medium is removed; (b) heating the sample obtained from step (a) in the presence of a divalent carboxylic acid compound to obtain a heat-treated sample comprising the tissue; (c) contacting the heat-treated sample from step (b) with an enzyme having a cell dispersion activity to separate the sample into individual cells, thereby obtaining the sample comprising the individual cells dispersed in a solvent; (d) subjecting the separated cells from step (c) to flow cytometry to obtain optical information; and (e) analyzing the cells based on the optical information obtained in the step (d).

摘要翻译： 提供了一种细胞分析方法，其包括（a）从醛固定并嵌入在水不溶性包埋介质中的组织标本获得的样品中除去包埋培养基，从而获得包含组织的样品，其中嵌入介质为 删除 （b）在二元羧酸化合物的存在下加热步骤（a）得到的样品，得到包含组织的热处理样品; （c）将来自步骤（b）的经热处理的样品与具有细胞分散活性的酶接触以将样品分离成单个细胞，从而获得包含分散在溶剂中的各个细胞的样品; （d）使来自步骤（c）的分离的细胞流式细胞仪获得光学信息; 和（e）基于步骤（d）中获得的光学信息来分析单元。

公开(公告)号：US09097630B2

公开(公告)日：2015-08-04

申请号：US14105355

申请日：2013-12-13

申请人： BioPath Automation, L.L.C.

发明人： Warren P. Williamson, IV ,  Craig B. Berky ,  Stephen P. Whitlatch ,  Thomas J. Ward

IPC分类号： G01N1/28 ,  G01N1/36 ,  B01L3/00 ,  B01L9/00 ,  G01N1/31

CPC分类号： G01N1/36 ,  B01L3/508 ,  B01L9/52 ,  B01L2300/043 ,  G01N2001/315 ,  G01N2001/366 ,  Y10T436/25

摘要： A method of staging a tissue sample cassette, including a cassette body and a lid, from an upper position in a frame used during a tissue embedding process to a lower position in the frame. The method comprises coupling the cassette with the frame such that the cassette is in the upper position and positively stopped at the upper position by first structure on a wall of the frame. The cassette is moved relative to the frame to the lower position by selectively engaging the cassette with a staging mechanism. An external force is applied to the cassette with the staging mechanism in a direction to move the cassette from the upper position toward the lower position. The cassette is retained in the lower position using second structure on the wall of the frame, such that the cassette is exposed for embedding the cassette in an embedding medium and subsequently slicing the tissue sample embedded in the embedding medium.

摘要翻译： 一种从在组织嵌入过程中使用的框架中的上部位置将组织样本盒（包括盒体和盖子）分层到框架中的下部位置的方法。 该方法包括将盒子与框架连接，使得盒子处于上部位置，并且通过框架壁上的第一结构在上部位置上被主动地停止。 通过选择性地将盒与分段机构接合，盒带相对于框架移动到下部位置。 一个外力作用在盒子上，其中分段机构沿一个使盒子从上部位置向下位置运动的方向。 使用框架壁上的第二结构将盒子保持在下部位置，使得盒子被暴露以将盒子嵌入到嵌入介质中，并随后切片嵌入在嵌入介质中的组织样本。

公开(公告)号：US20150104596A1

公开(公告)日：2015-04-16

申请号：US14356152

申请日：2011-11-03

申请人： Ulrich Felix Vogel

发明人： Ulrich Felix Vogel

IPC分类号： G09F3/00 ,  G01N1/36

CPC分类号： G09F3/0297 ,  B01L3/54 ,  B01L2300/021 ,  G01N1/06 ,  G01N1/36 ,  G01N2001/366 ,  Y10T428/13 ,  Y10T428/139 ,  Y10T428/1397 ,  Y10T428/24802

摘要： Prior art for processing human cell or tissue biopsy specimens implies the individualization of the specimens by marking the specimen containers (e.g. for transportation) and the specimen holders (e.g. plastic cassettes, glass slides) but not the specimen itself. The specimen processing steps include several manual transfers of the specimens between different containers and specimen holders. This manual transfer may result in specimen mix-ups. These specimen mix-ups can be prevented by attaching solvent resistant uni- or multicolored barcoded cuttable strips of cellulose acetate directly to the specimens thereby marking and individualizing the specimen itself. The strips adhere to the specimens at all steps of tissue processing and evaluation.

摘要翻译： 用于处理人细胞或组织活检标本的现有技术意味着通过标记样品容器（例如用于运输）和样品保持器（例如塑料盒，玻璃载玻片）而不是样品本身来个体化样品。 样品处理步骤包括在不同容器和样品架之间的样品的几个手动转移。 此手动传输可能导致样品混合。 可以通过将抗溶剂的单色或多色条形可转换的醋酸纤维素条直接粘贴到样品上，从而标记和个体化样品本身，可以防止这些样品混合。 在组织处理和评估的所有步骤中条带粘附到样品上。

公开(公告)号：US08283134B2

公开(公告)日：2012-10-09

申请号：US12086590

申请日：2006-12-25

申请人： Shigeru Amano ,  Yoshinobu Toda

发明人： Shigeru Amano ,  Yoshinobu Toda

IPC分类号： G01N1/30

CPC分类号： G01N1/36 ,  G01N2001/315 ,  G01N2001/366 ,  G01N2001/368

摘要： A cassette for fixing, embedding and slicing biological tissues includes a base sheet and a lid sheet formed of an elongated plastic sheet which is sliceable and has not less than predetermined strength. The base sheet has grooves as biological tissue receiving portions. The base plate and the lid sheet are formed by folding the plastic sheet along a fold line so that the lid sheet can be selectively opened and closed. Biological tissues are pressed against the bottoms of the grooves with pressing/retaining sponge members secured to the lid sheet at its portions corresponding to the respective grooves. In this state, the biological tissues are fixed by a fixing agent, and the cassette is dehydrated, infiltrated with paraffin as an embedding agent to embed the cassette, thereby forming a block including an embedding frame. The block is then sliced with a microtome to provide good biological tissue specimens.

摘要翻译： 用于固定，嵌入和切片生物组织的盒子包括基片和由可切割且具有不小于预定强度的细长塑料片形成的盖片。 基片具有作为生物组织接收部的槽。 基板和盖片通过沿折叠线折叠塑料片而形成，使得盖片可以选择性地打开和关闭。 生物组织被压靠在槽的底部，其压紧/保持海绵构件在其相应于各个槽的部分处固定到盖片。 在这种状态下，通过固定剂固定生物体组织，并将该盒脱水，用石蜡浸渍作为嵌入剂嵌入盒中，由此形成包括嵌入框架的块体。 然后用切片机将块切片以提供良好的生物组织标本。

公开(公告)号：US08080219B2

公开(公告)日：2011-12-20

申请号：US12425570

申请日：2009-04-17

申请人： James B. McCormick

发明人： James B. McCormick

IPC分类号： B01L3/00

CPC分类号： G01N1/36 ,  G01N1/31 ,  G01N1/312 ,  G01N2001/366 ,  Y10T436/2575

摘要： The present invention is directed to a card member for receiving a tissue specimen to be processed for histological examination including an absorbent portion for maintaining the tissue specimen in engageable contact with the card member, and a generally non-adhesive portion for allowing separation of the tissue specimen from the card member, where the card member provides the specimen with a known, consistent orientation throughout processing.

摘要翻译： 本发明涉及一种卡片构件，用于接收用于组织学检查的待加工组织样本，包括用于保持组织样本与卡片构件接合的吸收部分，以及用于允许组织分离的大体上非粘合部分 来自卡片构件的标本，其中卡片构件在整个加工过程中向标本提供已知的，一致的取向。

公开(公告)号：US08034292B2

公开(公告)日：2011-10-11

申请号：US13047053

申请日：2011-03-14

申请人： Douglas P. Allen ,  Dominic P. DiNovo ,  George A. Keller ,  Rebeccah P. Quam ,  Thomas J. Ward ,  Warren P. Williamson, IV

发明人： Douglas P. Allen ,  Dominic P. DiNovo ,  George A. Keller ,  Rebeccah P. Quam ,  Thomas J. Ward ,  Warren P. Williamson, IV

IPC分类号： G01N21/00

CPC分类号： G01N1/36 ,  G01N35/00732 ,  G01N2001/366 ,  Y10T436/2525 ,  Y10T436/2575

摘要： An automated machine for handling and embedding tissue samples contained on microtome sectionable supports. The machine includes an input member configured to hold a plurality of the microtome sectionable supports prior to a tissue embedding operation. An output member is configured to hold a plurality of the microtome sectionable supports after the tissue embedding operation. A cooling unit is configured to hold at least one of the microtome sectionable supports during the tissue embedding operation. A motorized carrier assembly is mounted for movement and configured to hold at least one of the microtome sectionable supports. The carrier assembly moves the support from the input member to the cooling unit and, finally, to the output member. A dispensing device dispenses an embedding material onto the microtome sectionable support and at least one tissue sample carried by the microtome sectionable support during the embedding operation.

摘要翻译： 一种用于处理和嵌入包含在切片机可切片支架上的组织样本的自动化机器。 该机器包括一个输入构件，构造成在组织嵌入操作之前保持多个切片机可切割支架。 输出构件被配置为在组织嵌入操作之后保持多个切片机可切割支撑件。 冷却单元构造成在组织嵌入操作期间保持切片机可分段支撑件中的至少一个。 安装电动载体组件用于移动并且构造成保持切片机可分段支撑件中的至少一个。 托架组件将支撑件从输入构件移动到冷却单元，最后移动到输出构件。 分配装置在嵌入操作期间将嵌入材料分配到切片机可切片支撑件和由切片机可切割支撑件携带的至少一个组织样本。

公开(公告)号：US20110182783A1

公开(公告)日：2011-07-28

申请号：US13047044

申请日：2011-03-14

申请人： Warren P. Williamson, IV ,  Thomas J. Ward ,  Stephen P. Whitlatch

发明人： Warren P. Williamson, IV ,  Thomas J. Ward ,  Stephen P. Whitlatch

IPC分类号： B01L3/00

CPC分类号： G01N1/36 ,  G01N35/00732 ,  G01N2001/366 ,  Y10T436/2525 ,  Y10T436/2575

摘要： An automated machine for handling and embedding tissue samples contained on microtome sectionable supports. The machine includes an input member configured to hold a plurality of the microtome sectionable supports prior to a tissue embedding operation. An output member is configured to hold a plurality of the microtome sectionable supports after the tissue embedding operation. A cooling unit is configured to hold at least one of the microtome sectionable supports during the tissue embedding operation. A motorized carrier assembly is mounted for movement and configured to hold at least one of the microtome sectionable supports. The carrier assembly moves the support from the input member to the cooling unit and, finally, to the output member. A dispensing device dispenses an embedding material onto the microtome sectionable support and at least one tissue sample carried by the microtome sectionable support during the embedding operation.

摘要翻译： 一种用于处理和嵌入包含在切片机可切片支架上的组织样本的自动化机器。 该机器包括一个输入构件，构造成在组织嵌入操作之前保持多个切片机可切割支架。 输出构件被配置为在组织嵌入操作之后保持多个切片机可切割支撑件。 冷却单元构造成在组织嵌入操作期间保持切片机可分段支撑件中的至少一个。 安装电动载体组件用于移动并且构造成保持切片机可分段支撑件中的至少一个。 托架组件将支撑件从输入构件移动到冷却单元，最后移动到输出构件。 分配装置在嵌入操作期间将嵌入材料分配到切片机可切片支撑件和由切片机可切割支撑件携带的至少一个组织样本。

公开(公告)号：US20110076753A1

公开(公告)日：2011-03-31

申请号：US12889843

申请日：2010-09-24

申请人： Peter Goerner ,  Caroline Breen ,  Roger Harrington Smith ,  Sheila Christine Dandy ,  Alan Harold Bridge

发明人： Peter Goerner ,  Caroline Breen ,  Roger Harrington Smith ,  Sheila Christine Dandy ,  Alan Harold Bridge

IPC分类号： C12M1/00

CPC分类号： G01N1/31 ,  G01N1/06 ,  G01N1/312 ,  G01N1/36 ,  G01N1/42 ,  G01N2001/364 ,  G01N2001/366

摘要： A histology tissue embedding apparatus including a reservoir for an embedding medium and an outlet valve connected to the reservoir through which the embedding medium is dispensable, and including trigger means which control the operation of the outlet valve, the trigger means having a rest position in which the outlet valve is closed, wherein the rest position of the trigger means in relation to the outlet valve is adjustable.

摘要翻译： 一种组织学组织包埋装置，其包括用于嵌入介质的储存器和连接到所述储存器的出口阀，所述储存器通过所述储存器嵌入介质是可分配的，并且包括控制所述出口阀的操作的触发装置，所述触发装置具有静止位置， 出口阀关闭，其中触发装置相对于出口阀的静止位置是可调节的。