公开(公告)号：US20170151322A9

公开(公告)日：2017-06-01

申请号：US14765365

申请日：2014-02-05

申请人： UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. ,  THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES ,  THERMO FISHER SCIENTIFIC INC.

发明人： Jon Michael Karpilow ,  Mark Steven Oberste ,  Ralph A. Tripp ,  Stephen M. Tompkins

IPC分类号： A61K39/13 ,  C12N7/00 ,  C12N15/113

CPC分类号： A61K39/13 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2320/12 ,  C12N2770/32021 ,  C12N2770/32034 ,  C12N2770/32052 ,  C12N2770/32351 ,  C12N2770/32651

摘要： Provided herein are engineered cell lines. In some embodiments, cells of an engineered cell line have altered expression of a gene and/or altered expression of an miRNA, wherein the altered expression results in increased or decreased production of a virus. The virus is a picomavirus, such as a poliovirus or Enterovirus 71. Also provided herein are methods for using the engineered cells to produce virus, and methods for treating a subject having or at risk of having a viral infection.

公开(公告)号：US09663485B2

公开(公告)日：2017-05-30

申请号：US14442637

申请日：2013-11-14

申请人： The United States of America, as represented by the Secretary, Dept. of Health and Human Services

发明人： Juan Jose Marugan ,  Wei Zheng ,  Jingbo Xiao ,  John McKew

IPC分类号： A01N43/16 ,  A61K31/355 ,  C07D311/58 ,  A61K31/335 ,  A61K31/35 ,  A61K31/352 ,  A61K31/353 ,  C07D311/72 ,  A61K31/122 ,  A61K31/724 ,  C07C50/02 ,  C07C50/06 ,  C07C50/24

CPC分类号： C07D311/58 ,  A61K31/122 ,  A61K31/335 ,  A61K31/35 ,  A61K31/352 ,  A61K31/353 ,  A61K31/355 ,  A61K31/724 ,  C07C50/02 ,  C07C50/06 ,  C07C50/24 ,  C07D311/72

摘要： The subject invention relates to improved tocopheryl quinone derivatives and tocopherol derivatives having improved pharmacokinetics in vivo that can, in some embodiments, be useful in the treatment of Lysosomal Storage Disorders, restoration of normal mitochondrial ATP production, modulation of intracellular calcium ion concentration and other treatments or therapies. The tocopheryl quinone derivatives and tocopherol derivatives have side chains that have terminally halogenated carbon atoms.

公开(公告)号：US20160368944A1

公开(公告)日：2016-12-22

申请号：US15255466

申请日：2016-09-02

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES

发明人： Genbin Shi ,  Gary X. Shaw ,  Xinhua Ji

IPC分类号： C07H19/16

CPC分类号： C07H19/16 ,  C07D475/04 ,  C07H19/167 ,  G16B15/00

摘要： The disclosure provides linked purine pterin compounds and analogues thereof that are novel HPPK inhibitors. The HPPK inhibitors described herein are compounds and the pharmaceutically acceptable salts thereof of general Formula I: (I). The variables, e.g. A1 to A3, R1 to R4, L1, L2, B1, and B2 are described herein. Compounds and salts of Formula I bind to HPPK with high affinity and specificity. Pharmaceutical compositions containing an HPPK inhibitor of Formula I and methods of treating a bacterial infection in a patient by providing one or more HPPK inhibitors of Formula I to the patient are also provided. Processes and intermediates useful for preparing compounds of Formula I are also provided. Methods of using the disclosed compounds to guide the development of additional novel anti-bacterial agents are also provided.

摘要翻译： 变量例如 A1〜A3，R1〜R4，L1，L2，B1，B2。 式I的化合物和盐以高亲和力和特异性结合HPPK。 还提供了含有式I的HPPK抑制剂和通过向患者提供一种或多种式I的HPPK抑制剂来治疗患者细菌感染的方法的药物组合物。 还提供了可用于制备式I化合物的方法和中间体。 还提供了使用所公开的化合物指导另外的新型抗菌剂的开发的方法。

公开(公告)号：US20160355897A1

公开(公告)日：2016-12-08

申请号：US15173450

申请日：2016-06-03

申请人： The United States of America, as represented by the Secretary, Dept. of Health and Human Services

发明人： William Allan Nix ,  M. Steven Oberste

IPC分类号： C12Q1/70

CPC分类号： C12Q1/701 ,  A61K2300/00 ,  A61K39/12 ,  A61K2039/6075 ,  C12N7/00 ,  C12N2770/32311 ,  C12N2770/32351 ,  C07K14/005 ,  C12Q1/70 ,  C12Q1/6806 ,  G01N33/56983

摘要： Disclosed herein are methods and compositions for detection of enterovirus D in a sample, particularly detection of enterovirus D68. The methods include contacting a sample with at least one primer (such as a forward primer and/or a reverse primer) capable of specifically amplifying an EV-D68 viral protein 1 (VP1) nucleic acid or a portion thereof and/or a detectably labeled probe capable of specifically hybridizing to an EV-D68 VP1 nucleic acid, under conditions sufficient for specific amplification of the EV-D68 VP1 nucleic acid by the at least one primer and/or under conditions sufficient for specific hybridization of the probe to the EV-D68 nucleic acid. The amplification of the EV-D68 VP1 nucleic acid and/or the hybridization of the probe to the EV-D68 VP1 nucleic acid is detected, thereby identifying presence of EV-D68 in the sample.

摘要翻译： 本文公开了用于检测样品中肠道病毒D的方法和组合物，特别是肠道病毒D68的检测。 所述方法包括将样品与能够特异性扩增EV-D68病毒蛋白1（VP1）核酸或其部分和/或可检测标记的至少一个引物（例如正向引物和/或反向引物）接触 能够在足以用于通过至少一个引物特异性扩增EV-D68 VP1核酸的条件下和/或在足以使探针与EV- D68 VP1核酸特异性杂交的条件下，与EV-D68 VP1核酸特异性杂交的探针， D68核酸。 检测EV-D68 VP1核酸的扩增和/或探针与EV-D68 VP1核酸的杂交，从而鉴定样品中EV-D68的存在。

公开(公告)号：US09512429B2

公开(公告)日：2016-12-06

申请号：US14886970

申请日：2015-10-19

申请人： The United States of America, as represented by the Secretary, Dept. of Health and Human Services

发明人： John Milburn Jessup ,  Nikolay Korokhov

IPC分类号： C07H21/02 ,  C07H21/04 ,  A61K48/00 ,  C12N15/63 ,  C12N15/113 ,  C12N15/86

CPC分类号： C12N15/113 ,  C12N15/86 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/32 ,  C12N2710/10032 ,  C12N2710/10043 ,  C12N2710/10045 ,  C12N2799/022 ,  C12N2799/028 ,  C12N2820/007 ,  C12N2830/008

摘要： The present description relates to an inhibitory RNA molecule, comprising an oligonucleotide that selectively knocks down expression a Nanog pseudogene expressed in many human cancers, a replicating viral vector capable of encoding such inhibitory RNA molecule, pharmaceutical compositions comprising said vector, and methods of treating cancer by administration of said pharmaceutical composition.

摘要翻译： 本说明书涉及抑制性RNA分子，其包含选择性敲低在许多人类癌症中表达的Nanog假基因表达的寡核苷酸，能够编码该抑制性RNA分子的复制型病毒载体，包含所述载体的药物组合物和治疗癌症的方法 通过施用所述药物组合物。

公开(公告)号：US20160311910A1

公开(公告)日：2016-10-27

申请号：US15101583

申请日：2014-10-30

申请人： The United States of America, as represented by the Secretary, Dept. of Health and Human Services

发明人： Haiying Qin ,  Terry J. Fry

IPC分类号： C07K16/28 ,  C07K14/725 ,  C07K14/715 ,  C07K16/30 ,  C07K14/705

摘要： The invention provides a chimeric antigen receptor (CAR) comprising an antigen binding domain specific for TSLPR, a transmembrane domain, and an intracellular T cell signaling domain. Nucleic acids, recombinant expression vectors, host cells, populations of cells, antibodies, or antigen binding portions thereof, and pharmaceutical compositions relating to the CARs are disclosed. Methods of detecting the presence of a proliferative disorder, e.g., cancer, in a mammal and methods of treating or preventing a proliferative disorder, e.g., cancer, in a mammal are also disclosed.

摘要翻译： 本发明提供了包含对TSLPR特异性的抗原结合结构域，跨膜结构域和细胞内T细胞信号结构域的嵌合抗原受体（CAR）。 公开了核酸，重组表达载体，宿主细胞，细胞群，抗体或其抗原结合部分以及与CAR相关的药物组合物。 还公开了在哺乳动物中检测哺乳动物中增殖性疾病例如癌症的存在的方法以及治疗或预防哺乳动物中增殖性疾病例如癌症的方法。

公开(公告)号：US20160231310A1

公开(公告)日：2016-08-11

申请号：US14915602

申请日：2014-09-02

申请人： UNIVERSITY OF MARYLAND, COLLEGE PARK ,  CHILDREN'S NATIONAL MEDICAL CENTER ,  THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPT., OF HEALTH AND HUMAN SERVICES

发明人： Omar Bilal AYYUB ,  Adam Michael BEHRENS ,  Peter KOFINAS ,  Marshall Lynn SUMMAR ,  Juan Manuel CABRERA-LUQUE ,  Gary CUNNINGHAM ,  Anton SIMEONOV ,  Juan MARUGAN

IPC分类号： G01N33/49 ,  G01N33/68 ,  G01N27/327

CPC分类号： G01N33/4925 ,  G01N21/78 ,  G01N27/327 ,  G01N27/3275 ,  G01N33/6893 ,  G01N33/84 ,  G01N2800/04

摘要： The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

摘要翻译： 本公开涉及能够在离聚物存在下使用靛酚试剂测量一种或多种氨或铵离子的总浓度的生物传感器。 在一些实施方案中，生物传感器包括含氟化膜，其包含离子交换体，其接触构造成接收诸如全血的样品的容器中的碱性缓冲液。 本公开还涉及在护理生物传感器的点处检测或定量全血中的氨或铵离子浓度的方法，而不依赖于气相色谱法或任何需要大约二十分钟的测量。

公开(公告)号：US09402889B2

公开(公告)日：2016-08-02

申请号：US14585721

申请日：2014-12-30

申请人： The United States of America, as represented by the Secretary, Dept. of Health and Human Services

发明人： Dennis J. Kopecko ,  De-Qi Xu

IPC分类号： A61K39/112 ,  C07H21/02 ,  C07H21/04 ,  A61K48/00 ,  C07K14/25 ,  C12N15/52 ,  A61K39/00

CPC分类号： A61K39/0283 ,  A61K48/00 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/25 ,  C12N15/52 ,  Y02A50/484

摘要： The invention relates to Salmonella typhi Ty21a comprising core-linked Shigella dysenteriae serotype 1 O-specific polysaccharide (O-Ps) and DNA encoding O antigen biosynthesis, said DNA selected from the group consisting of: a) the DNA sequence set out in any one of SEQ ID NOs: 1 and 2 and species homologs thereof; b) DNA encoding Shigella dysenteriae serotype 1 polypeptides encoded by any one of SEQ ID NOs: 1 and 2, and species homologs thereof; and c) DNA encoding a O antigen biosynthesis gene product that hybridizes under moderately stringent conditions to the DNA of (a) or (b); and related sequences, compositions of matter, vaccines, methods of using, and methods of making.

公开(公告)号：US20160184242A1

公开(公告)日：2016-06-30

申请号：US15065355

申请日：2016-03-09

申请人： The United States of America, as Represented by the Secretary, Dept. of Health and Human Services

发明人： Raj K. Puri ,  Bharat H. Joshi ,  Benjamin Rubin

IPC分类号： A61K31/145 ,  A61K45/06 ,  A61K9/48 ,  A61K9/00 ,  A61K9/28

CPC分类号： A61K31/145 ,  A61K9/0053 ,  A61K9/28 ,  A61K9/4891 ,  A61K45/06 ,  A61K2300/00

摘要： The present disclosure is directed to methods for inhibiting or suppressing metastasis of a tumor in a mammalian subject using a cysteamine product, e.g., cysteamine or cystamine or derivatives thereof. Also described herein is a method for treating pancreatic cancer in a mammalian subject by administering a cysteamine product described herein.

摘要翻译： 本公开涉及使用半胱胺产物（例如半胱胺或胱胺或其衍生物）抑制或抑制哺乳动物受试者中肿瘤转移的方法。 本文还描述了通过施用本文所述的半胱胺产物来治疗哺乳动物受试者的胰腺癌的方法。

公开(公告)号：US20160169899A1

公开(公告)日：2016-06-16

申请号：US14903706

申请日：2014-07-11

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES

发明人： Majda HAZNADAR ,  Ewy MATHE ,  Andrew D. PATTERSON ,  Curtis Craig HARRIS ,  Frank GONZALEZ ,  Kristopher KRAUSZ ,  Soumen MANN

IPC分类号： G01N33/574

CPC分类号： G01N33/57423 ,  G01N33/5743 ,  G01N33/57488 ,  G01N2400/00 ,  G01N2800/52

摘要： The present invention provides methods and materials for diagnosing cancer in an individual using a tissue, blood or urine sample from the patient. Specifically, the disclosed method comprises determining the level of one or more metabolite selected from the group consisting of creatine riboside, metabolite 561+, Cortisol sulfate and N-acetylneuraminic acid. The present invention also provides a method for determining the prognosis of a cancer patient by determining the level of one or more metabolite selected from the group consisting of creatine riboside, metabolite 561+, Cortisol sulfate and N-acetylneuraminic acid. Also provided are kits for detecting cancer or determining the prognosis of a cancer patient.

摘要翻译： 本发明提供了使用来自患者的组织，血液或尿液样本诊断个体癌症的方法和材料。 具体地，所公开的方法包括测定选自肌酸核苷，代谢物561+，硫酸皮质醇和N-乙酰神经氨酸的一种或多种代谢物的水平。 本发明还提供了通过测定选自肌酸核苷，代谢物561+，硫酸皮质醇和N-乙酰神经氨酸的一种或多种代谢物的水平来确定癌症患者的预后的方法。 还提供了用于检测癌症或确定癌症患者的预后的试剂盒。