公开(公告)号：US12131805B2

公开(公告)日：2024-10-29

申请号：US16428656

申请日：2019-05-31

Applicant: 10X Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Serge Saxonov ,  Kevin Ness ,  Benjamin Hindson

IPC: G16B30/10 ,  G16B30/00 ,  G16B30/20

CPC classification number: G16B30/10 ,  G16B30/00 ,  G16B30/20

Abstract: The invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention improves the worth and significance of sequencing data.

公开(公告)号：US12098423B2

公开(公告)日：2024-09-24

申请号：US16736323

申请日：2020-01-07

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20240191271A1

公开(公告)日：2024-06-13

申请号：US18388735

申请日：2023-11-10

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Zahra Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

CPC classification number: C12P19/34 ,  C12Q1/6806 ,  C40B20/04 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US12005454B2

公开(公告)日：2024-06-11

申请号：US17145858

申请日：2021-01-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Debkishore Mitra ,  Donald A. Masquelier ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N2035/00237 ,  G01N35/085 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US20230335220A1

公开(公告)日：2023-10-19

申请号：US18198763

申请日：2023-05-17

Applicant: 10x Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Serge Saxonov ,  Kevin Ness ,  Benjamin Hindson

IPC: G16B30/10 ,  G16B30/00 ,  G16B30/20

CPC classification number: G16B30/10 ,  G16B30/00 ,  G16B30/20

Abstract: The invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention improves the worth and significance of sequencing data.

公开(公告)号：US20220290217A1

公开(公告)日：2022-09-15

申请号：US17312615

申请日：2019-12-06

Applicant: 10x Genomics, Inc.

Inventor： Lucas Frenz ,  Preyas Shah ,  Benjamin Hindson ,  Eswar Prasad Ramachandran Iyer

IPC: C12Q1/6837 ,  C12N15/10

Abstract: Methods for determining a location of a feature on an array include: (a) providing a first array with a first plurality of features immobilized on a first substrate; (b) providing a second array with a second plurality of features immobilized on a second substrate; (c) aligning the first array with the second array; (d) hybridizing a first barcoded oligonucleotide of the first array to a second barcoded oligonucleotide of the second array, thereby producing a combined nucleic acid that includes first and second spatial barcodes; (e) determining all or a portion of the sequence of the combined nucleic acid; and (f) identifying the second barcoded oligonucleotide associated with the first barcoded oligonucleotide in the combined nucleic acid, and determining the location of a second feature in the second array.

公开(公告)号：US11359239B2

公开(公告)日：2022-06-14

申请号：US17314526

申请日：2021-05-07

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Kevin Ness ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20220098659A1

公开(公告)日：2022-03-31

申请号：US17392610

申请日：2021-08-03

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20210285044A1

公开(公告)日：2021-09-16

申请号：US16818437

申请日：2020-03-13

Applicant: 10X Genomics, Inc.

Inventor： Paul Ryvkin ,  Jason Underwood ,  Michael Schnall-Levin ,  Tarjei Mikkelsen ,  Benjamin Hindson

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6851

Abstract: Provided herein are compositions, systems and methods for tagging molecular events, reactions, species, etc., but without the need for complex, highly diverse libraries of tagging molecules. Provided are tagging moieties that can have a smaller number, a few, or even a single original “tagging” structure that may be transformed or transformable, in situ, into a collection of larger numbers of unique tagging or “barcode” moieties.

公开(公告)号：US11035002B2

公开(公告)日：2021-06-15

申请号：US16998425

申请日：2020-08-20

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.