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    Modified variant bowman birk protease inhibitors
    93.
    发明授权
    Modified variant bowman birk protease inhibitors 有权
    改良型bowman birk蛋白酶抑制剂

    公开(公告)号：US07803902B2

    公开(公告)日：2010-09-28

    申请号：US12252062

    申请日：2008-10-15

    申请人： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

    发明人： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

    IPC分类号： C07K14/00

    CPC分类号： C07K14/811 ,  A61K8/64 ,  A61Q7/02

    摘要： The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.

    摘要翻译： 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白（BBPI），并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体，表达和产生修饰变体BBPI的转化宿主细胞，修饰变体BBPI蛋白，包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。

    Thermostable Neutral Metalloproteases
    95.
    发明申请
    Thermostable Neutral Metalloproteases 有权
    耐热中性金属蛋白酶

    公开(公告)号：US20090263882A1

    公开(公告)日：2009-10-22

    申请号：US12473735

    申请日：2009-05-28

    申请人： Andrew Shaw ,  Louise Wallace ,  David A. Estell ,  Ronaldus W. J, Hommes ,  Sang-Kyu Lee ,  Hiroshi Oh ,  Eugene S. Sadlowski

    发明人： Andrew Shaw ,  Louise Wallace ,  David A. Estell ,  Ronaldus W. J, Hommes ,  Sang-Kyu Lee ,  Hiroshi Oh ,  Eugene S. Sadlowski

    IPC分类号： C12N9/50

    CPC分类号： C12N9/54 ,  C11D1/02 ,  C11D3/386 ,  C11D3/38663 ,  C12Y304/24

    摘要： The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

    摘要翻译： 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中，本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中，中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁，漂白和消毒的应用。

    Methods For Selective Targeting
    96.
    发明申请
    Methods For Selective Targeting 有权
    选择性定位方法

    公开(公告)号：US20090093393A1

    公开(公告)日：2009-04-09

    申请号：US12099632

    申请日：2008-04-08

    申请人： Christopher J. Murray ,  Pilar Tijerina ,  David A. Estell ,  Yiyou Chen

    发明人： Christopher J. Murray ,  Pilar Tijerina ,  David A. Estell ,  Yiyou Chen

    IPC分类号： C11D1/10 ,  C40B30/04 ,  C07K7/00

    CPC分类号： G01N33/54393 ,  C07K7/06 ,  C07K7/08 ,  C12N15/1034 ,  C12N15/1037 ,  C12N15/1072 ,  C40B40/02 ,  G01N33/6845 ,  G01N2500/02

    摘要： A selective targeting method is disclosed comprising contacting a library of ligands, particularly a peptide library, with an anti-target to allow the ligands to bind to the anti-target; separating the non-binding ligands from the anti-target bound ligands, contacting the non-binding anti-target ligands with a target allowing the unbound ligands to bind with the target to form a target-bound ligand complex; separating the target-bound ligand complex from ligands which do not bind to the target, and identifying the target-bound ligands on the target-bound ligand complex wherein the target-bound ligands have a KD in the range of about 10−7 to 10−10 M. Additionally claimed are the ligands identified according to the method.

    摘要翻译： 公开了一种选择性靶向方法，其包括将配体文库，特别是肽文库与抗靶相接触，以允许配体结合抗靶; 将非结合配体与抗靶结合配体分离，使非结合抗靶配体与允许未结合配体与靶结合以形成靶结合配体复合物的靶; 将靶结合的配体络合物与不结合靶的配体分离，并鉴定靶结合的配体配合物上的靶结合配体，其中靶结合配体的KD在约10-7至10范围内 另外要求的是根据该方法鉴定的配体。

    Serine proteases from gram-positive microorganisms
    99.
    发明授权
    Serine proteases from gram-positive microorganisms 失效

    公开(公告)号：US07329527B2

    公开(公告)日：2008-02-12

    申请号：US11014432

    申请日：2004-12-16

    申请人： David A. Estell

    发明人： David A. Estell

    IPC分类号： C12N9/48 ,  C12N9/14 ,  C12P21/04 ,  C07H21/04 ,  C11D3/02

    CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38663 ,  C12N9/18 ,  C12N9/48 ,  C12N15/75

    摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.

    Serine proteases from-gram-positive microorganisms
    100.
    发明授权
    Serine proteases from-gram-positive microorganisms 失效
    来自革兰氏阳性微生物的丝氨酸蛋白酶

    公开(公告)号：US07329526B2

    公开(公告)日：2008-02-12

    申请号：US11014339

    申请日：2004-12-16

    申请人： David A. Estell

    发明人： David A. Estell

    IPC分类号： C12N9/48 ,  C12N9/14 ,  C12P21/04 ,  C07H21/04 ,  C11D3/02

    CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38663 ,  C12N9/18 ,  C12N9/48 ,  C12N15/75

    摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.

    摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。