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240 条记录 (耗时 0.027 秒)

    METHOD OF ENRICHING AND DETECTING A TARGET MICROORGANISM
    92.
    发明申请
    METHOD OF ENRICHING AND DETECTING A TARGET MICROORGANISM 审中-公开

    公开(公告)号：US20180258458A1

    公开(公告)日：2018-09-13

    申请号：US15756100

    申请日：2016-08-29

    申请人： 3M INNOVATIVE PROPERTIES COMPANY

    发明人： GREGORY W. SITTON

    IPC分类号： C12Q1/04 ,  G01N33/58 ,  G01N21/64

    CPC分类号： C12Q1/04 ,  C12Q1/10 ,  C12Q2304/22 ,  C12Q2304/24 ,  G01N21/6428 ,  G01N33/583

    摘要： The present disclosure provides a method of detecting a target bacterium. The method includes forming a primary enrichment culture that includes a test sample, a primary enrichment medium and a redox dye; incubating the primary enrichment culture for a first period of time equal to eight hours or less; assessing the primary enrichment culture during the first period of time to detect whether the redox dye changes from a first state to a second state; if the second state is detected in the primary enrichment culture during the first period of time, mixing a portion of the primary enrichment culture with a secondary enrichment medium to form a secondary enrichment culture and incubating the secondary enrichment culture for a second period of time; and performing a test to detect the target bacterium using a portion of the second enrichment culture.

    Quenching dye for labeling biomolecules and method for preparing the same
    96.
    发明授权
    Quenching dye for labeling biomolecules and method for preparing the same 有权
    用于标记生物分子的淬灭染料及其制备方法

    公开(公告)号：US09512315B2

    公开(公告)日：2016-12-06

    申请号：US15033478

    申请日：2013-10-31

    申请人： DKC CORPORATION

    发明人： Jin Woo Park ,  Kiwon Kim

    IPC分类号： C09B62/45 ,  G01N33/58

    CPC分类号： C09B62/45 ,  C07C309/53 ,  C09B56/12 ,  C09B62/445 ,  G01N33/582 ,  G01N33/583

    摘要： The present disclosure provides a quenching dye with an anthraquinone structure, which has a broad and high absorption spectrum in the visible and near-infrared wavelength regions and exhibits high stability and superior solubility in aqueous conditions. A quenching dye composition containing the anthraquinone compound according to the present disclosure as an active ingredient can be widely used in the field of optical molecular imaging to observe the movement of a biomolecule because it can easily label a biomolecule manipulated in an aqueous buffer.

    摘要翻译： 本公开内容提供了具有蒽醌结构的淬灭染料，其在可见光和近红外波长区域具有宽而高的吸收光谱，并且在水性条件下表现出高稳定性和优异的溶解性。 包含根据本公开的蒽醌化合物作为活性成分的淬灭染料组合物可以广泛用于光学分子成像领域，以观察生物分子的移动，因为它可以容易地标记在水性缓冲液中操作的生物分子。

    QUENCHING DYE FOR LABELING BIOMOLECULES AND METHOD FOR PREPARING THE SAME
    97.
    发明申请
    QUENCHING DYE FOR LABELING BIOMOLECULES AND METHOD FOR PREPARING THE SAME 有权
    用于标记生物分子的叮咬染料及其制备方法

    公开(公告)号：US20160266130A1

    公开(公告)日：2016-09-15

    申请号：US15033478

    申请日：2013-10-31

    申请人： DKC CORPORATION

    发明人： Jin Woo PARK ,  Kiwon KIM

    IPC分类号： G01N33/58

    CPC分类号： C09B62/45 ,  C07C309/53 ,  C09B56/12 ,  C09B62/445 ,  G01N33/582 ,  G01N33/583

    摘要： The present disclosure provides a quenching dye with an anthraquinone structure, which has a broad and high absorption spectrum in the visible and near-infrared wavelength regions and exhibits high stability and superior solubility in aqueous conditions. A quenching dye composition containing the anthraquinone compound according to the present disclosure as an active ingredient can be widely used in the field of optical molecular imaging to observe the movement of a biomolecule because it can easily label a biomolecule manipulated in an aqueous buffer.

    摘要翻译： 本公开内容提供了具有蒽醌结构的淬灭染料，其在可见光和近红外波长区域具有宽而高的吸收光谱，并且在水性条件下表现出高稳定性和优异的溶解性。 包含根据本公开的蒽醌化合物作为活性成分的淬灭染料组合物可以广泛用于光学分子成像领域，以观察生物分子的移动，因为它可以容易地标记在水性缓冲液中操作的生物分子。

    METHOD AND KIT FOR ASSESSING VIABLE CELLS
    99.
    发明申请
    METHOD AND KIT FOR ASSESSING VIABLE CELLS 审中-公开
    用于评估可变细胞的方法和工具包

    公开(公告)号：US20160131658A1

    公开(公告)日：2016-05-12

    申请号：US14821096

    申请日：2015-08-07

    申请人： ChemoMetec A/S

    发明人： Mette Elena Skindersø ,  Helle Frøbose Sørensen ,  Søren Kjaerulff

    IPC分类号： G01N33/58 ,  G01N21/64

    CPC分类号： G01N33/582 ,  C09B15/00 ,  C09B57/00 ,  C09B57/02 ,  G01N21/6428 ,  G01N33/502 ,  G01N33/583 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2510/00

    摘要： The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).

    摘要翻译： 本发明提供用于分析细胞的简单，快速的方法和程序，在此对细胞进行定量和定性评估，例如活力。 本发明涉及各种任选取代的报告化合物在完整（例如，活的）细胞中与在非完整（例如死亡，应激和凋亡）细胞中比较高的浓度存在的硫醇类物质反应时特别可检测的用途） 。 本发明还涉及各种任选取代的报告化合物在与完整和/或未完整细胞中存在的物质反应时特别可检测的用途。 此外，本发明涉及与使用各种任选取代的报告化合物的测量技术和/或仪器的用途。 本发明还涉及用于分析细胞的方法中使用的组合物，例如包含N-（7-二甲基氨基-4-甲基-3-香豆素基） - 马来酰亚胺（DACM）的组合物。

    COMPOSITIONS AND METHODS FOR MAKING AND USING OXYGEN SENSING NANOFIBERS AND SCAFFOLDS
    100.
    发明申请
    COMPOSITIONS AND METHODS FOR MAKING AND USING OXYGEN SENSING NANOFIBERS AND SCAFFOLDS 有权
    制备和使用氧气感应纳米纤维和组分的组合物和方法

    公开(公告)号：US20160041177A1

    公开(公告)日：2016-02-11

    申请号：US14818980

    申请日：2015-08-05

    申请人： University of Virginia Patent Foundation

    发明人： Kenneth Brayman ,  Daniel Bowers ,  Cassandra L. Fraser ,  Edward A. Botchwey, III

    IPC分类号： G01N33/58 ,  A61K49/00

    CPC分类号： G01N33/582 ,  A61K49/0015 ,  A61K49/0021 ,  A61K49/0054 ,  A61K49/0095 ,  G01N33/583 ,  G01N33/84 ,  G01N2800/7038

    摘要： To address the need for scaffold-based oxygen concentration monitoring, a single-component, self-referenced oxygen sensor was made into nanofibers. Electrospinning process parameters were tuned to produce a biomaterial scaffold with specific morphological features. The ratio of an oxygen sensitive phosphorescence signal to an oxygen insensitive fluorescence signal was calculated at each image pixel to determine an oxygenation value. A single component boron dye-polymer conjugate was chosen for additional investigation due to improved resistance to degradation in aqueous media compared to a dye polymer blend. Standardization curves show that in fully supplemented media, the fibers are responsive to dissolved oxygen concentrations less than 15 parts per million. Spatial and temporal ratiometric gradients were observed in vitro radiating outward from the center of a dense adherent cell grouping. Sensor activation in ischemia and cell transplant models in vivo show oxygenation decreases on the scale of minutes.

    摘要翻译： 为了满足基于脚手架氧浓度监测的需要，将单组分自参考氧传感器制成纳米纤维。 调整电纺工艺参数以产生具有特定形态特征的生物材料支架。 在每个图像像素处计算氧敏感磷光信号与不敏感荧光信号的比率，以确定氧合值。 选择单组分硼染料 - 聚合物共轭物进行额外的研究，因为与染料聚合物共混物相比，水性介质中的降解性改善。 标准化曲线显示，在完全补充的培养基中，纤维对低于15ppm的溶解氧浓度有反应。 体外观察到空间和时间比例梯度，从密集贴壁细胞组的中心向外辐射。 局部缺血和细胞移植模型中的传感器活化在体内显示出氧合作用以分钟的规模降低。