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    Real time electronic cell sensing system and applications for cell-based assays
    94.
    发明授权
    Real time electronic cell sensing system and applications for cell-based assays 有权
    实时电子传感系统和基于细胞的测定应用

    公开(公告)号：US08026080B2

    公开(公告)日：2011-09-27

    申请号：US11725040

    申请日：2007-03-15

    Applicant: Xiaobo Wang ,  Yama A. Abassi ,  Xiao Xu ,  Jiangbo Gan

    Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Xiao Xu ,  Jiangbo Gan

    IPC: C12Q1/20 ,  C12Q1/08 ,  C12M1/34

    CPC classification number: G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

    Abstract: The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

    Abstract translation: 本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。 在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。 在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件 用于控制设备站和阻抗分析仪。 在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。 在一些优选的方面，设计测定法以研究化合物对细胞的影响，例如细胞毒性测定。 在其它优选方面，设计这些测定法来研究影响细胞对抗原的IgE介导的反应的化合物。

    Device and method for the detection and enumeration of multiple groups of microorganisms
    95.
    发明授权
    Device and method for the detection and enumeration of multiple groups of microorganisms 有权
    用于检测和计数多组微生物的装置和方法

    公开(公告)号：US07745169B2

    公开(公告)日：2010-06-29

    申请号：US11766208

    申请日：2007-06-21

    Applicant: Gideon Eden ,  Ruth Eden

    Inventor： Gideon Eden ,  Ruth Eden

    IPC: C12Q1/08 ,  C12N1/02 ,  C12M1/34

    CPC classification number: C12M41/36 ,  C12Q1/06

    Abstract: A device and method simultaneously detects and enumerates two groups of microorganisms in a test sample, utilizing a single test container. In the container liquid growth media, a chromogenic substrate and a fluorogenic substrate are mixed with the test sample. The test container is incubated to allow bacterial growth and metabolism. Spectral changes of the substrates are dynamically detected using two external light sources aimed at a transparent section of the test container, and a single external photo detector. One light source operates in the visible band and the second in the long ultraviolet band. The two dynamic time patterns generated by the two substrates are analyzed in real time to determine the presence or absence of each microorganisms group and to enumerate their original concentrations in the test sample.

    Abstract translation: 一种装置和方法同时利用单个测试容器检测和列举测试样品中的两组微生物。 在容器液体生长培养基中，将显色底物和荧光底物与测试样品混合。 将测试容器温育以允许细菌生长和代谢。 使用瞄准测试容器的透明部分的两个外部光源和单个外部光电检测器来动态地检测衬底的光谱变化。 一个光源在可见光带中工作，第二个在长紫外线带中工作。 实时分析由两个底物产生的两个动态时间图，以确定每个微生物组的存在或不存在，并列举其在测试样品中的原始浓度。

    Method and Kit for Detecting Components in a Sample
    96.
    发明申请
    Method and Kit for Detecting Components in a Sample 有权
    用于检测样品中组分的方法和试剂盒

    公开(公告)号：US20080269064A1

    公开(公告)日：2008-10-30

    申请号：US11587710

    申请日：2004-04-29

    Applicant: Marc Ramael

    Inventor： Marc Ramael

    IPC: C40B30/04 ,  C12Q1/08

    CPC classification number: G01N33/587 ,  G01N33/54373 ,  G01N33/58

    Abstract: Methods and a kit for use in the detection of a component in a sample on a solid support are disclosed which use a conjugate and polymer having metal particles of diameter in the nanometer range (that is between 0.1 and 500 nm). Methods and a kit for detection of a component in a sample on a solid support which have a conjugate and optionally a polymer bound to one or more supermagnetic particles are also disclosed. The methods and kits may be used for enhancing in vivo imaging and microscopy.

    Abstract translation: 公开了一种用于检测固体支持物上的样品中组分的试剂盒，其使用直径在纳米范围内（即在0.1至500nm之间）的金属颗粒的共轭物和聚合物。 还公开了用于检测固体支持物上具有缀合物和任选的与一个或多个超磁性颗粒结合的聚合物的样品中组分的试剂盒。 方法和试剂盒可用于增强体内成像和显微镜。

    APPARATUS AND METHOD FOR TESTING LIQUID SAMPLES
    97.
    发明申请
    APPARATUS AND METHOD FOR TESTING LIQUID SAMPLES 有权
    用于测试液体样品的装置和方法

    公开(公告)号：US20080003638A1

    公开(公告)日：2008-01-03

    申请号：US11852540

    申请日：2007-09-10

    Applicant: Ross Goldman ,  David Townsend ,  Kenneth Smith ,  Kathleen McCarthy

    Inventor： Ross Goldman ,  David Townsend ,  Kenneth Smith ,  Kathleen McCarthy

    IPC: C12Q1/06 ,  C12Q1/08

    CPC classification number: B01L3/50853 ,  B01L3/502 ,  B01L2200/0642 ,  B01L2300/042 ,  B01L2300/0832 ,  B01L2300/0864 ,  C12M41/36 ,  C12Q1/06

    Abstract: An article for holding a liquefied sample for the quantification of biological material in the sample includes a device having a reaction chamber enclosing a volume therein, the reaction chamber having an upper opening through which a liquefied sample can be poured and a plurality of discrete non-permeable compartments, each of the compartments having an upper rim and being configured and dimensioned to hold separate aliquots of a liquefied sample therein; and a gasket lid removably secured to the top of the device, the gasket lid being configured and dimensioned for sealing the upper rim of each compartment to prevent liquid communication between the compartments.

    Abstract translation: 用于保持用于定量样品中的生物材料的液化样品的物品包括具有包围其中的体积的反应室的装置，所述反应室具有上部开口，液体样品可以通过该上部开口被排出， 每个隔室具有上边缘，并且其构造和尺寸被设计成在其中容纳液化样品的分离的等分试样; 以及可移除地固定到装置的顶部的垫圈盖，垫圈盖被构造和尺寸设计成用于密封每个隔室的上边缘以防止隔室之间的液体连通。

    Method for identifying Listeria monocytogenes and culture medium
    98.
    发明授权
    Method for identifying Listeria monocytogenes and culture medium 有权
    确定单核细胞增多性李斯特菌和培养基的方法

    公开(公告)号：US07270978B2

    公开(公告)日：2007-09-18

    申请号：US10432048

    申请日：2001-11-19

    Applicant: Céline Roger-Dalbert ,  Laurence Barbaux

    Inventor： Céline Roger-Dalbert ,  Laurence Barbaux

    IPC: C12Q1/00 ,  C12Q1/04 ,  C12Q1/08

    CPC classification number: C12N1/20 ,  C12N9/16 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q2334/50

    Abstract: A substrate which can be used for the direct identification of pathogenic bacteria belonging to the genus Listeria by detecting an esterase activity other than Phosphatidyl Inositol-specific Phospholipase C (PI-PLC), an esterase which is specific to the species Listeria monocytogenes. The use of two substrates, one as described above and the other specific for all or some members of the genus Listeria, and a reaction medium containing such a substrate or such a combination of substrates are disclosed. An identification method which exploits such culture media is also disclosed. The invention is particularly applicable in the field of diagnosis.

    Abstract translation: 可用于通过检测除磷脂酰肌醇特异性磷脂酶C（PI-PLC）以外的酯酶活性来直接鉴定属于利斯特氏菌属的致病菌的底物，其是对单核细胞增生利斯特氏菌属特异性的酯酶。 公开了使用如上所述的两个底物和另一个特异于李斯特氏菌的所有或一些成员的底物，以及含有这种底物或底物组合的反应介质。 还公开了利用这种培养基的识别方法。 本发明特别适用于诊断领域。

    Rapid method of detection and enumeration of sulfide-producing bacteria in food products
    99.
    发明申请
    Rapid method of detection and enumeration of sulfide-producing bacteria in food products 审中-公开
    食品中硫化物生产细菌的快速检测和计数方法

    公开(公告)号：US20050221419A1

    公开(公告)日：2005-10-06

    申请号：US11133084

    申请日：2005-05-19

    Applicant: Grete lorentzen ,  Olaug Skjerdal ,  James Berg ,  Olaug Skjerdal ,  Groa Loraitzar

    Inventor： Grete lorentzen ,  Olaug Skjerdal ,  James Berg ,  Olaug Skjerdal ,  Groa Loraitzar

    IPC: C12Q1/04 ,  C12Q1/06 ,  C12Q1/08

    CPC classification number: C12Q1/04 ,  C12Q1/06 ,  Y10S435/968

    Abstract: A rapid method for detecting spoilage of a food sample, particularly a fish sample, by detecting and enumerating sulfide-producing bacteria (SPB). A growth medium containing iron and sulfur is combined with the food sample forming an incubation mixture which is incubated for a period of time. In one embodiment, a plurality of fluorescence measurements are taken during an incubation period of about 3 hours to 17 hours at 30° C. SPB are determined to be present in the sample if the fluorescence measurement initially increases and then decreases to form a fluorescence maximum (peak). The time to detection of the fluorescence peak can be used with a correlation schedule to enumerate the SPB in the food sample. In another embodiment, a visual test can also be used to identify color changes in the incubation mixture to provide a semi-quantitative enumeration of SPB effective after about 3 hours to 17 hours of incubation.

    Abstract translation: 通过检测和列举产生硫化物的细菌（SPB）来检测食品样品，特别是鱼类样品的变质的快速方法。 将包含铁和硫的生长培养基与形成孵育混合物的食物样品合并一段时间。 在一个实施方案中，在30℃下约3小时至17小时的孵育期间进行多次荧光测量。如果荧光测量最初增加然后降低以形成荧光最大值，则确定SPB存在于样品中 （峰）。 检测荧光峰的时间可以与相关性计划一起使用以列举食品样品中的SPB。 在另一个实施方案中，视觉测试也可以用于鉴定孵育混合物中的颜色变化，以提供在约3小时至17小时孵育后有效的SPB的半定量计数。

    Gelled system and method for detecting microorganisms by separation and culture on gelled system
    100.
    发明授权
    Gelled system and method for detecting microorganisms by separation and culture on gelled system 失效
    凝胶体系和通过分离和培养在凝胶体系上检测微生物的方法

    公开(公告)号：US6020150A

    公开(公告)日：2000-02-01

    申请号：US913757

    申请日：1997-09-22

    Applicant: Genevieve Contant-Pussard ,  Fran.cedilla.oise Boisard-Beaupere ,  Jean-Luc Martinoli ,  Alain Rousseau

    Inventor： Genevieve Contant-Pussard ,  Fran.cedilla.oise Boisard-Beaupere ,  Jean-Luc Martinoli ,  Alain Rousseau

    IPC: C12M1/00 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/24 ,  C12N1/02 ,  C12Q1/06 ,  C12Q1/08

    CPC classification number: C12Q1/04 ,  C12Q1/24 ,  Y10S435/975

    Abstract: A method for detecting the presence or absence of microorganisms belonging to the group which consists of bacteria and yeasts in a liquid sample (5) of a biological material. The method comprises placing the liquid sample (5) in a centrifuge tube (6a, 6b) above a gelled system (10) comprising at least (a) a first so-called development phase (1), i.e. a gel comprising a microorganism culture medium and a reagent for inducing a detectable optical measurement change in the presence of microorganisms, said gel being an intimate mixture of water and water-absorbing polymeric particles that have swelled in such a way that, in said intimate mixture, said polymeric particles have (.alpha.) a dry weight concentration of 0.05-0.2 g/ml, and (.beta.) a swollen-state diameter of 90-320 .mu.m, the water in the intimate mixture being at least partially provided by said culture medium; centrifuging; and revealing the presence or absence of microorganisms in said liquid sample (5) at said first phase (1) of the gelled system (10) by means of said reagent inducing a detectable optical measurement change.

    Abstract translation: PCT No.PCT / FR96 / 00419 Sec。 371日期：1997年9月22日 102（e）日期1997年9月22日PCT提交1996年3月20日PCT公布。 公开号WO96 / 29427 日期1996年9月26日在生物材料的液体样品（5）中检测属于由细菌和酵母组成的组的微生物的存在或不存在的方法。 该方法包括将液体样品（5）放置在凝胶系统（10）上方的离心管（6a，6b）中，所述凝胶系统（10）至少包含（a）第一所谓的显影相（1），即包含微生物培养物 介质和用于在微生物存在下诱导可检测的光学测量变化的试剂，所述凝胶是水和吸水聚合物颗粒的紧密混合物，其以这样的方式溶胀，使得在所述紧密混合物中所述聚合物颗粒具有 α）0.05-0.2g / ml的干重浓度，和（β）90-320μm的膨胀状态直径，紧密混合物中的水至少部分地由所述培养基提供; 离心; 并且通过所述试剂在凝胶系统（10）的所述第一阶段（1）处揭示所述液体样品（5）中存在或不存在微生物，从而引起可检测的光学测量变化。

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