公开(公告)号：US09551697B2

公开(公告)日：2017-01-24

申请号：US14056795

申请日：2013-10-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen

IPC: G01N27/447 ,  G01N33/487 ,  G01N27/327

CPC classification number: G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.

Abstract translation: 公开了鉴定分子的方法。 通过在第一周期期间将一个第一电压信号施加到一对电极上，将一个分子吸引到纳米孔，其中第一电压信号引起通过纳米孔的第一离子电流，其指示分子的一部分的性质接近于 纳米孔。 在第二时段期间，通过在所述一对电极施加第二电压信号，从所述纳米孔释放所述分子，其中所述第二电压信号引起通过所述纳米孔的第二离子电流。 至少部分地基于通过包含第一离子电流和第二离子电流的纳米孔的净离子电流确定第一周期和第二周期。

公开(公告)号：US09494554B2

公开(公告)日：2016-11-15

申请号：US13918626

申请日：2013-06-14

Applicant: Genia Technologies, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Kevin Deierling

IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/68

CPC classification number: G01N27/44791 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/631

Abstract: The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. Nucleic acid molecules can be sequenced with a high accuracy (e.g., greater than 97% in a single pass) using a chip comprising an array of independently addressable nanopore sensors at a density of at least about 500 sites per 1 mm2. An individual nanopore sensor can include a nanopore in a membrane that is adjacent or in proximity to a sensing electrode.

Abstract translation: 本公开提供了用于测序核酸分子的装置，系统和方法。 使用包含独立可寻址的纳米孔传感器阵列的芯片，密度为每1mm 2至少约500个位点，核酸分子可以以高精度（例如，单次通过中大于97％）进行测序。 单个纳米孔传感器可以包括邻近或接近检测电极的膜中的纳米孔。

公开(公告)号：US20160266064A1

公开(公告)日：2016-09-15

申请号：US14977312

申请日：2015-12-21

Applicant: Genia Technologies, Inc.

Inventor： Kevin Deierling ,  Roger J.A. Chen ,  David J. Fullagar

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487

Abstract: A device for controlling, detecting, and measuring a molecular complex is disclosed. The device comprises a common electrode. The device further comprises a plurality of measurement cells. Each measurement cell includes a cell electrode and an integrator electronically coupled to the cell electrode. The integrator measures the current flowing between the common electrode and the cell electrode. The device further comprises a plurality of analog-to-digital converters, wherein an integrator from the plurality of measurement cells is electrically coupled to one analog-to-digital converter of the plurality of analog-to-digital converters.

公开(公告)号：US09393541B2

公开(公告)日：2016-07-19

申请号：US14794524

申请日：2015-07-08

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen

IPC: G05D23/00 ,  B01J19/00 ,  G05D23/20

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.

公开(公告)号：US20160123923A1

公开(公告)日：2016-05-05

申请号：US14534042

申请日：2014-11-05

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

Abstract translation: 公开了一种在基于纳米孔的测序芯片上输出纳米孔传感器测量的方法。 测量与纳米孔传感器相关的电特性。 处理与纳米孔传感器相关的电特性。 确定电特性和一个或多个先前电特性的总结。 导出了电气特性和一个或多个以前的电气特性的总结。 确定总结包括确定电特性和一个或多个先前的电特性的至少一部分对应于纳米孔传感器处的基本呼叫事件。 总结表示电特性和一个或多个先前电特性的至少一部分。

公开(公告)号：US09322062B2

公开(公告)日：2016-04-26

申请号：US14521427

申请日：2014-10-22

Applicant: Genia Technologies, Inc.

Inventor： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech ,  Hui Tian ,  Robert Yuan ,  John Foster ,  Roger Chen

IPC: G01N27/327 ,  C12Q1/68 ,  C12M1/34

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

Abstract translation: 公开了用于分子检测和感测的生物芯片。 生物芯片包括基底。 生物芯片包括形成在衬底上的多个离散位置，其密度大于每平方毫米500个孔。 每个离散部位包括设置在基底上以形成孔的侧壁。 每个离散位置包括设置在井的底部的电极。 在一些实施例中，形成井，使得井之间的串扰减少。 在一些实施例中，设置在阱的底部的电极被组织成电极组，其中每组电极共享公共对电极。 在一些实施例中，设置在阱底部的电极具有专用的对电极。 在一些实施例中，侧壁的表面被硅烷化，使得表面有助于在孔中或邻近孔的膜的形成。

公开(公告)号：US20150275287A1

公开(公告)日：2015-10-01

申请号：US14225263

申请日：2014-03-25

Applicant: Genia Technologies, Inc.

Inventor： Hui Tian

IPC: C12Q1/68

CPC classification number: G01N33/48721

Abstract: A nanopore based sequencing chip is disclosed. The sequencing chip comprises a first portion made from a first wafer. The first portion includes an array of nanopore cells. The first portion further includes a measurement circuit connected to one or more nanopore cells, the measurement circuit producing an output measurement signal. The first portion further includes one or more vias transmitting the output measurement signal. The sequencing chip further includes a second portion made from a second wafer, the second portion comprising one or more corresponding vias receiving the output measurement signal.

Abstract translation: 公开了一种基于纳米孔的测序芯片。 测序芯片包括由第一晶片制成的第一部分。 第一部分包括纳米孔细胞阵列。 第一部分还包括连接到一个或多个纳米孔单元的测量电路，该测量电路产生输出测量信号。 第一部分还包括传输输出测量信号的一个或多个通孔。 排序芯片还包括由第二晶片制成的第二部分，第二部分包括接收输出测量信号的一个或多个对应的通孔。

公开(公告)号：US09121826B2

公开(公告)日：2015-09-01

申请号：US14558222

申请日：2014-12-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen

IPC: H01L23/552 ,  G01N27/447 ,  H01L21/768

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

公开(公告)号：US20150153303A1

公开(公告)日：2015-06-04

申请号：US14558268

申请日：2014-12-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/327

CPC classification number: G01N27/3278 ,  C12Q1/6874 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to: receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.

Abstract translation: 公开了一种用于检测分子复合物的电性能的系统。 该系统包括电耦合到分子复合物的电极，其输出受分子复合物的电特性影响的电信号，其中分子复合物的电特性对电信号的影响的特征在于期望的带宽。 该系统还包括积分放大器电路，其被配置为：从电极接收电信号。 积分放大器电路还被配置为在预定带宽内随时间选择性地放大和积分电信号的一部分，其中至少部分地基于预期带宽来选择预定带宽。

公开(公告)号：US09041420B2

公开(公告)日：2015-05-26

申请号：US13893142

申请日：2013-05-13

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Randy Davis

IPC: C12Q1/00 ,  G01N27/447 ,  C12Q1/68 ,  G01N15/00 ,  B82Y30/00

CPC classification number: C12Q1/6816 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N27/3278 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  G01N2015/0038 ,  Y10T29/49002 ,  Y10T29/49117 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.

Abstract translation: 用于表征分子的技术在本文中描述。 在一个实例中，分子的一部分被捕获在纳米孔中，跨越纳米孔施加可变电压，直到分子的被捕获部分在纳米孔内移动，并且基于影响运动所需的电刺激来表征分子 纳米孔内的分子的截留部分的至少一部分。