公开(公告)号：US20180136159A1

公开(公告)日：2018-05-17

申请号：US15820032

申请日：2017-11-21

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  J. William Maney

IPC: G01N27/22 ,  G01N33/487 ,  C12Q1/6869 ,  G01N27/447

CPC classification number: G01N27/228 ,  C12Q1/6869 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

公开(公告)号：US09806033B2

公开(公告)日：2017-10-31

申请号：US15240872

申请日：2016-08-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen

IPC: H01L23/552 ,  G01N27/447 ,  H01L21/768 ,  G01N33/487 ,  H01L23/522 ,  H01L23/58 ,  H01L49/02 ,  B82Y15/00

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

公开(公告)号：US09789460B2

公开(公告)日：2017-10-17

申请号：US15183560

申请日：2016-06-15

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen

IPC: G05D23/00 ,  B01J19/00 ,  G05D23/20

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.

公开(公告)号：US20170268052A1

公开(公告)日：2017-09-21

申请号：US15444042

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Preethi Sarvabhowman ,  Charles Schwab

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6806 ,  C12Y207/07007 ,  C12Q2565/631

Abstract: The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.

公开(公告)号：US09759711B2

公开(公告)日：2017-09-12

申请号：US13759701

申请日：2013-02-05

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  David J. Fullagar

IPC: G01N27/327 ,  G01N33/487

CPC classification number: G01N33/48721

Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

公开(公告)号：US20170199150A1

公开(公告)日：2017-07-13

申请号：US15371106

申请日：2016-12-06

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  J. William Maney, Jr.

IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/68

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

公开(公告)号：US20170198343A1

公开(公告)日：2017-07-13

申请号：US15385779

申请日：2016-12-20

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH

IPC: C12Q1/68 ,  G01N27/327 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

公开(公告)号：US20170091381A1

公开(公告)日：2017-03-30

申请号：US14864408

申请日：2015-09-24

Applicant: Genia Technologies, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  Bill Maney ,  Jayalakshmi Rajaraman

IPC: G06F19/22

CPC classification number: G06F19/22 ,  G01N33/48721

Abstract: A system includes a plurality of nanopore cells. Data corresponding to nanopore states of the plurality of nanopore cells is received. The data is analyzed to determine a compressed output size of the data given at least one compression technique. It is determined whether the compressed output size exceeds a data budget. In the event it is determined that the compressed output size exceeds the data budget, the data is modified. The modified data is outputted.

公开(公告)号：US20170088890A1

公开(公告)日：2017-03-30

申请号：US15274770

申请日：2016-09-23

Applicant: Genia Technologies, Inc.

Inventor： Timothy Kellogg CRAIG ,  Cynthia Ann CECH ,  Michael DORWART ,  Liv Elisabeth JENSEN ,  Marshall Winston PORTER ,  Christos TZITZILONIS ,  Alexander Hyun-min YANG

IPC: C12Q1/68 ,  C07K14/31

CPC classification number: C12Q1/6869 ,  C07K14/31 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

公开(公告)号：US20170043314A1

公开(公告)日：2017-02-16

申请号：US15183560

申请日：2016-06-15

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: B01J19/00 ,  G05D23/20

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.

Abstract translation: 公开了一种用于调节测量阵列的温度的系统。 该系统包括包括多个传感器的测量阵列，其中多个传感器集成在集成电路管芯上。 该系统包括集成在集成电路管芯上的热传感器，其中热传感器感测与多个传感器相关联的温度。 该系统还包括耦合到集成电路管芯的热泵，其中热泵由包括热传感器的反馈控制电路控制。