公开(公告)号：US11602709B2

公开(公告)日：2023-03-14

申请号：US16328640

申请日：2017-08-29

Applicant: EMD Millipore Corporation

Inventor： Joseph Geringer ,  Robert Castro ,  Martin Szyk ,  Sophie Daudenarde ,  Joseph Almasian

IPC: B01D46/42 ,  B01D46/00

Abstract: Port protection device particularly suited for an encapsulated filter unit. In certain embodiments, the protection device comprises a main body defining an internal region configured to receive a port or the like protruding from an encapsulated filter. In certain embodiments, the main body includes a slot that allows for easy removal of the protection device from the port or the like. A tube management system may be provided to receive and hold tubing. One suitable tube management system includes two spaced tangs that extend from the main body of the protection device and are configured to cooperatively receive and hold tubing between them.

公开(公告)号：US20220402968A1

公开(公告)日：2022-12-22

申请号：US17756096

申请日：2020-12-11

Applicant: EMD Millipore Corporation

Inventor： Herbert Lutz ,  Kenneth Scott ,  Michael Bruce ,  Kristina Cunningham

IPC: C07K1/36 ,  C12M1/00 ,  C12N7/00 ,  C07K1/22 ,  C07K1/34 ,  B01D15/38 ,  B01D15/18 ,  B01D15/36 ,  B01D15/32

Abstract: Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to filtration to form a clarified product sample; subjecting said clarified product sample to affinity chromatography to remove impurities; subsequently subjecting said product sample to diafiltration followed by virus filtration and optional concentration. The buffer used during the diafiltration step (and thus in the virus filtration step) is the buffer desired for the final formulation of the product.

公开(公告)号：USD970517S1

公开(公告)日：2022-11-22

申请号：US29758166

申请日：2020-11-12

Applicant: Sigma-Aldrich Co. LLC ,  EMD Millipore Corporation

Designer： Sarah Trice ,  Lindsey Rickershauser ,  Xiang Wu ,  Churl Oh ,  Nadia Brugger ,  Emma Gardener

公开(公告)号：USD962282S1

公开(公告)日：2022-08-30

申请号：US29758161

申请日：2020-11-12

Applicant: Sigma-Aldrich Co. LLC ,  EMD Millipore Corporation

Designer： Sarah Trice ,  Lindsey Rickershauser ,  Xiang Wu ,  Churl Oh ,  Nadia Brugger ,  Emma Gardener

公开(公告)号：US20220186259A1

公开(公告)日：2022-06-16

申请号：US17605190

申请日：2020-06-25

Applicant: EMD Millipore Corporation

Inventor： Naohisa Yoshioka

IPC: C12N15/86 ,  C12N9/12

Abstract: Synthetic, self-replicating RNA vectors comprising sequence encoding at least one immortalization protein, and use of the self-replicating RNA vectors to extend the lifespan of primary cell populations and expand said populations of primary cells.

公开(公告)号：US20220186235A1

公开(公告)日：2022-06-16

申请号：US17606644

申请日：2020-05-13

Applicant: EMD Millipore Corporation

Inventor： Naohisa Yoshioka

IPC: C12N15/79 ,  C12N15/10 ,  C12N9/22 ,  C12N15/11

Abstract: Synthetic, noninfectious, self-replicating RNA vectors that encode CRISPR proteins are provided. Each self-replicating RNA vector comprises a sequence encoding a plurality of non-structural replication complex proteins from an alphavirus and a sequence encoding a CRISPR protein. Also provided are methods for genome editing in which a synthetic self-replicating RNA vector is transfected into cells along with at least one corresponding guide RNA.

公开(公告)号：US20220176278A1

公开(公告)日：2022-06-09

申请号：US17682221

申请日：2022-02-28

Applicant: EMD Millipore Corporation

Inventor： Jonathan Steen ,  Mikhail Kozlov ,  Herbert Lutz ,  Joseph Parrella ,  Elizabeth M. Goodrich ,  Bala Raghunath

IPC: B01D29/90 ,  B01D61/14 ,  B01D63/04 ,  B01D63/12 ,  B01D29/52 ,  B01D29/60

Abstract: Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

公开(公告)号：US11305271B2

公开(公告)日：2022-04-19

申请号：US15677526

申请日：2017-08-15

Applicant: EMD Millipore Corporation

Inventor： David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

IPC: B01D15/36 ,  B01J20/28 ,  B01J47/12 ,  B01J39/26 ,  C12N7/02 ,  B01J47/014 ,  B01J47/127 ,  B01D15/38 ,  C07K1/18 ,  B01J41/20 ,  B01D15/32 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  C07K16/00 ,  C12N7/00 ,  G01N30/52

Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

公开(公告)号：US20220032235A1

公开(公告)日：2022-02-03

申请号：US17432122

申请日：2020-02-20

Applicant: EMD Millipore Corporation

Inventor： Kevin Rautio ,  Sean Foley ,  Stephen G. Hunt ,  Bumchul Lee ,  Nathan Landry

IPC: B01D63/08

Abstract: Single-use integral chromatography unit having an inlet and an outlet, and comprising one or more plates or pairs of filter plates interposable between a pair of end plates. In certain embodiments, each of the filter plates comprises a polymeric framework with one or more membranes supported therein. The filter plates and end plates may be assembled to form a substantially fixed, substantially water-tight, integral stack. Fluid entering the unit through a common inlet passes the membrane or membranes of each filter plate substantially contemporaneously prior to exiting the unit through a common outlet (cf, “parallel” flow). The assembly is a modular design, as multiple pairs of plates can be stacked in a suitable holder to form a single chromatography unit.

公开(公告)号：US20210388036A1

公开(公告)日：2021-12-16

申请号：US17388090

申请日：2021-07-29

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Neil Soice ,  Shari Spector ,  Kara Levine

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32

Abstract: The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.