公开(公告)号：US08871437B2

公开(公告)日：2014-10-28

申请号：US13133709

申请日：2009-12-09

Applicant: Tan Inoue ,  Hirohide Saito ,  Shunichi Kashida ,  Karin Hayashi

Inventor： Tan Inoue ,  Hirohide Saito ,  Shunichi Kashida ,  Karin Hayashi

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/63 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12N15/111 ,  C12N2310/14 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2320/50

Abstract: An object of the present invention is to provide an RNAi control system using an RNA-protein interaction motif. The present invention provides an shRNA comprising: a guide strand having a sequence complementary to a target sequence; a passenger strand which forms a duplex with the guide strand; and a linker strand which links the guide strand and the passenger strand, wherein the linker strand comprises an RNP-derived protein-binding motif sequence. The present invention also provides an RNAi control system comprising: the shRNA; and an RNP-derived protein which specifically binds to a protein-binding motif sequence in the shRNA.

Abstract translation: 本发明的目的是提供使用RNA-蛋白质相互作用基序的RNAi控制系统。 本发明提供了一种shRNA，其包含：具有与靶序列互补的序列的引导链; 与引导链形成双相的乘客股; 和连接引物链和乘客链的连接体链，其中连接体链包含RNP衍生的蛋白质结合基序序列。 本发明还提供了一种RNAi控制系统，其包含：shRNA; 以及特异性结合shRNA中蛋白质结合基序序列的RNP衍生蛋白质。

公开(公告)号：US08865124B2

公开(公告)日：2014-10-21

申请号：US12920283

申请日：2009-02-27

Applicant: Atsushi Miyawaki ,  Kazuki Sasaki

Inventor： Atsushi Miyawaki ,  Kazuki Sasaki

IPC: A61K51/00 ,  A61M36/14 ,  G01N33/50 ,  A61K51/08 ,  A61K49/14 ,  A61K49/00 ,  C07K14/00 ,  C12Q1/28 ,  G01N21/64 ,  G01N33/84 ,  G01N21/77

CPC classification number: G01N33/5008 ,  A61K49/0017 ,  A61K49/0056 ,  A61K49/14 ,  A61K51/08 ,  A61K51/088 ,  C07K14/00 ,  C12Q1/28 ,  G01N21/6428 ,  G01N21/77 ,  G01N33/5014 ,  G01N33/84 ,  G01N2500/10

Abstract: The present invention relates to fluorescent or luminescent probe reagents for measuring oxidative stress in a cell or an organism. Examples of the probe reagents include: a fluorescent or luminescent protein and a marker protein; a fluorescent or luminescent protein, a marker protein, and a regulatory factor; or a fluorescent or luminescent protein, a marker protein, a cleavage sequence, and a regulatory factor. In the probe reagents, the marker protein makes it possible to detect the oxidative stress caused by reactive oxygen species and comprises a regulatory factor-binding site and a ubiquitin-binding site; and the regulatory factor is a protein making it possible to regulate degradation of the marker protein in response to the reactive oxygen species. The present invention also relates to a method of measuring oxidative stress in a cell or an organism, or a method of screening a substance which suppresses or promotes the oxidative stress in a cell or an organism by using the probe reagent.

Abstract translation: 本发明涉及用于测量细胞或生物体中的氧化应激的荧光或发光探针试剂。 探针试剂的实例包括：荧光或发光蛋白和标记蛋白; 荧光或发光蛋白，标记蛋白和调节因子; 或荧光或发光蛋白，标记蛋白，切割序列和调节因子。 在探针试剂中，标记蛋白使得可以检测由活性氧引起的氧化应激，并且包含调节因子结合位点和泛素结合位点; 并且调节因子是使得可以响应于活性氧来调节标记蛋白的降解的蛋白质。 本发明还涉及一种测定细胞或生物体中的氧化应激的方法，或者通过使用探针试剂筛选抑制或促进细胞或生物体中的氧化应激的物质的方法。

公开(公告)号：US08791261B2

公开(公告)日：2014-07-29

申请号：US13255739

申请日：2010-03-10

Applicant: Makoto Fujita ,  Sota Sato ,  Kosuke Suzuki

Inventor： Makoto Fujita ,  Sota Sato ,  Kosuke Suzuki

IPC: C07F15/00 ,  C07H23/00

CPC classification number: C07H15/26 ,  C01B33/18 ,  C01F7/021 ,  C01G1/02 ,  C01G23/047 ,  C01G23/053 ,  C01G25/02 ,  C01P2002/85 ,  C01P2002/86 ,  C01P2002/89 ,  C01P2004/04 ,  C07H23/00

Abstract: Provided is a polyhedral transition metal complex including a hollow shell, the hollow shell including n1 (where n1 is an integer from 6 to 60) transition metal atoms and 2(n1) bidentate organic ligands, the bidentate organic ligands including a group derived from a polyhydroxy compound via a linking group, and formed so that the group derived from the polyhydroxy compound is oriented toward an inner space of the hollow shell. Also provided are: an ultrafine particle-containing transition metal complex including the polyhedral transition metal complex, and ultrafine particles of a metal oxide, the ultrafine particles being included within the hollow shell of the polyhedral transition metal complex; a method of producing the same. The invention thus provides a polyhedral transition metal complex that makes it possible to efficiently produce metal oxide particles having a uniform particle size of several nanometers, an ultrafine particle-containing polyhedral transition metal complex in which metal oxide particles are included within a polyhedral structure and the method of producing the same.

Abstract translation: 提供了包含中空壳体的多面体过渡金属络合物，中空壳体包括n1（其中n1为6至60的整数）过渡金属原子和2（n1）二齿有机配体，二齿有机配体包括衍生自 多羟基化合物通过连接基团形成，并且形成为使得来自多羟基化合物的基团朝向中空壳体的内部空间。 还提供：包含多面体过渡金属络合物的超细颗粒的过渡金属络合物和金属氧化物的超细颗粒，超细颗粒包含在多面体过渡金属络合物的中空壳内; 其制造方法。 因此，本发明提供了一种多面体过渡金属络合物，其可以有效地生产具有几纳米的均匀粒径的金属氧化物颗粒，其中在多面体结构内包含金属氧化物颗粒的超细颗粒的多面体过渡金属络合物， 制作方法

公开(公告)号：US08772181B2

公开(公告)日：2014-07-08

申请号：US14001171

申请日：2012-02-27

Applicant: Suguru Noda ,  Soichiro Takano

Inventor： Suguru Noda ,  Soichiro Takano

IPC: H01L21/469 ,  H01L29/08

CPC classification number: H01L21/28506 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/184 ,  H01L29/43 ,  H01L29/66742 ,  H01L29/78603 ,  H01L29/78684

Abstract: A production method for producing graphene on a substrate, and the like are provided. According to the method, in a forming step heating is conducted to a solid solution temperature at which a solid solution of carbon dissolved in a metal is able to be formed, and a solid solution layer (505) composed of the solid solution on a substrate (103) is formed; and in a removing step graphene (102) is grown on the substrate (103) by removing the metal from the solid solution layer (505) while maintaining the heating to the solid solution temperature. As a solvent for dissolving carbon a metal composed of a single element as well as various alloys are applicable. The graphene (102) touches directly the substrate (103), by removing the metal from the solid solution layer (505) by supplying an etching gas.

Abstract translation: 提供了在基板上制造石墨烯的制造方法等。 根据该方法，在成形步骤中，将能够形成溶解在金属中的碳的固体溶液的固溶温度加热至在基板上固溶的固溶体（505） （103）; 并且在去除步骤中，通过从固溶层（505）中除去金属，同时保持加热至固溶体温度，在基板（103）上生长石墨烯（102）。 作为溶解碳的溶剂，可以使用由单一元素构成的金属以及各种合金。 石墨烯（102）通过提供蚀刻气体从固溶体层（505）中除去金属，直接接触衬底（103）。

公开(公告)号：US20140183169A1

公开(公告)日：2014-07-03

申请号：US14201324

申请日：2014-03-07

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Junichi FUJITA

IPC: G01R3/00

CPC classification number: G01R3/00 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y35/00 ,  H01J37/317 ,  H01J2237/316 ,  H01J2237/3165

Abstract: A sharpened metallic probe having a top of main body including a carbon-containing metal and a method and an apparatus for fabricating the metallic probe. A metallic probe with an extremely sharpened tip is obtained by inducing a field emission from the tip of the main body of the metallic probe where a nanotube is joined thereto, heating and melting locally the tip of the main body of the metallic probe by a Joule heating, and tearing the nanotube toward a counter electrode by a Coulomb attractive force.

Abstract translation: 一种具有包括含碳金属的主体顶部的金属探针和用于制造金属探针的方法和装置。 通过从金属探针的主体的尖端引起场发射，其中纳米管被接合到其上，通过焦耳将金属探针的主体的尖端局部加热和熔化，获得具有极尖锐尖的金属探针 通过库仑吸引力加热和撕开纳米管朝向对电极。

公开(公告)号：US08765384B2

公开(公告)日：2014-07-01

申请号：US10590045

申请日：2005-02-18

Applicant: Yasuo Suda

Inventor： Yasuo Suda

IPC: G01N33/53 ,  G01N33/532 ,  G01N33/553

CPC classification number: G01N33/52 ,  C07H15/04 ,  G01N33/531 ,  G01N2400/00

Abstract: A novel ligand conjugate which is effectively utilizable for analyzing a function of a protein; a ligand-supporting object; and a method of analyzing a protein. The ligand conjugate has a structure which comprises: a linker compound having a structure represented by the following General Formula (1): (wherein n and p each is an integer of 0 to 6) in which X is a structure comprising one, two, or three hydrocarbon derivative chains which have an aromatic amino group at the end and may have a carbon-nitrogen bond in the main chain, Y is a hydro-carbon structure containing one or more sulfur atoms, and Z is a straight-chain structure comprising a carbon-carbon bond or carbon-oxygen bond; and a sugar which has a reducing end and is bonded to the linker compound through the aromatic amino group.

Abstract translation: 一种新颖的配体结合物，可有效用于分析蛋白质的功能; 配体支持物; 和分析蛋白质的方法。 配体缀合物具有以下结构：包含具有以下通式（1）表示的结构的连接体化合物：（其中n和p各自为0〜6的整数），其中，X为包含1〜 或在末端具有芳香族氨基并且在主链中可具有碳 - 氮键的三个烃衍生物链，Y是含有一个或多个硫原子的氢碳结构，Z是直链结构，包括 碳 - 碳键或碳 - 氧键; 和具有还原性末端并通过芳香族氨基与连接体化合物键合的糖。

公开(公告)号：US08746994B2

公开(公告)日：2014-06-10

申请号：US13272546

申请日：2011-10-13

Applicant: Norihisa Hasimoto

Inventor： Norihisa Hasimoto

IPC: G03B17/00 ,  H04N7/00 ,  H04N5/232

CPC classification number: H04N5/23238 ,  F16M11/046 ,  F16M11/10 ,  F16M11/2064 ,  F16M11/2085 ,  F16M11/2092 ,  G03B17/561 ,  G03B37/02

Abstract: So that parallax-free images can be taken even if the camera is rotated during filming and the direction of the camera is changed to an arbitrary direction, the auxiliary device is provided with a camera body retaining part and, in an equidistant surface centered on the nodal point of the camera, a camera attachment part. Regardless of the angle the camera makes as a result of the way in which the camera attachment part is mounted to a camera support mechanism part, the nodal point of the camera remains at a fixed location.

Abstract translation: 因此，即使照相机在拍摄期间旋转并且照相机的方向改变为任意方向，也可以获得无视差的图像，辅助设备设置有照相机主体保持部分，并且以等距离的表面为中心 相机的节点，相机附件部分。 由于照相机安装部件安装在照相机支撑机构部分上的方式，相机的角度无论如何，照相机的节点保持在固定位置。

公开(公告)号：US08722196B2

公开(公告)日：2014-05-13

申请号：US12921348

申请日：2009-03-02

Applicant: Shinji Yae ,  Tatsuya Hirano ,  Hitoshi Matsuda

Inventor： Shinji Yae ,  Tatsuya Hirano ,  Hitoshi Matsuda

IPC: B32B3/00 ,  B32B3/06 ,  B32B3/26 ,  B32B5/22 ,  B32B9/06 ,  B32B15/04

CPC classification number: H01L21/288 ,  C23C18/1619 ,  C23C18/1644 ,  C23C18/1889 ,  C23C18/31 ,  C23C18/44 ,  C23C18/54 ,  Y10T428/249953 ,  Y10T428/249955 ,  Y10T428/249977 ,  Y10T428/249978 ,  Y10T428/249979 ,  Y10T428/249986 ,  Y10T428/249994

Abstract: Proposed are a composite material having a high adhesiveness, wherein non-penetrating pores that are formed in a silicone surface layer are filled up with a metal or the like without leaving any voids by using the plating technique and the silicone surface layer is coated with the metal or the like, and a method of producing the composite material. A composite material, which has a high adhesiveness between a second metal or an alloy of the second metal (106a, 106b) and a silicone surface, can be obtained by filling up non-penetrating pores that are formed in the surface of a silicone substrate (100) substantially with a second metal or an alloy of the second metal (106a) with the use of the autocatalytic electroless plating technique wherein a first metal located at the bottom of the non-penetrating pores as described above serves as the starting point, and coating the surface of the silicone substrate (100) with the second metal (106b).

Abstract translation: 提出具有高粘合性的复合材料，其中形成在硅氧烷表面层中的非穿透孔用金属等填充，而不会通过使用电镀技术留下任何空隙，并且硅氧烷表面层涂覆有 金属等，以及复合材料的制造方法。 通过填充在硅树脂基材的表面上形成的非穿透孔，可以获得在第二金属或第二金属（106a，106b）的合金与硅酮表面之间具有高粘附性的复合材料 （100）基本上具有第二金属或第二金属（106a）的合金，使用自催化无电镀技术，其中位于如上所述的非穿透孔底部的第一金属作为起点， 以及用所述第二金属（106b）涂覆所述硅树脂基底（100）的表面。

公开(公告)号：US08666733B2

公开(公告)日：2014-03-04

申请号：US12997252

申请日：2009-06-03

Applicant: Kazuo Toraichi ,  Mitsuteru Nakamura ,  Yasuo Morooka

Inventor： Kazuo Toraichi ,  Mitsuteru Nakamura ,  Yasuo Morooka

IPC: G10L21/00 ,  G10L25/90 ,  G10L19/00

CPC classification number: G10L19/0208

Abstract: When encoding an audio signal, it is possible to efficiently encode the audio signal while maintaining high register signal components, and prevent deterioration of sound quality of decoded signal. A digital audio signal is divided into a plurality of frequency bands. The digital audio signal having been divided into each band is function-approximated for each divided band. Further, parameters of function having been function-approximated are encoded. When performing decoding process, parameters of the function of each band are used to perform function interpolation, synthesize the function-interpolated signal of each band interpolated, and decode the signal. Thus, when function-approximating each band, by suitably setting the function equation, it is possible to perform an encoding process while maintaining the high register components and perform a compression-coding process which enables reproduction with very good sound quality.

Abstract translation: 当对音频信号进行编码时，可以在保持高的寄存器信号分量的同时有效地编码音频信号，并且防止解码信号的声音质量的劣化。 数字音频信号被分成多个频带。 被划分成每个频带的数字音频信号对于每个分频带进行功能近似。 此外，对功能近似的功能的参数进行编码。 当执行解码处理时，使用每个频带的功能参数进行功能插值，合成插入的每个频带的功能插值信号，并对信号进行解码。 因此，当功能近似每个频带时，通过适当地设置函数方程，可以在保持高寄存器分量的同时进行编码处理，并执行能够以非常好的音质再现的压缩编码处理。

公开(公告)号：US08648223B2

公开(公告)日：2014-02-11

申请号：US13753628

申请日：2013-01-30

Applicant: Japan Science and Technology Agency ,  Nippoh Chemicals Co., Ltd.

Inventor： Junichi Yoshida ,  Seiji Suga ,  Kazuhide Kataoka ,  Koji Midorikawa ,  Yuji Hagiwara

IPC: C07C22/00

CPC classification number: C07C17/02 ,  C07B39/00 ,  C07C17/12 ,  C07C37/62 ,  C07C41/22 ,  C07C209/74 ,  C07C253/30 ,  C25B1/24 ,  C25B3/06 ,  C07C25/18 ,  C07C25/22 ,  C07C211/52 ,  C07C255/50 ,  C07C39/27 ,  C07C43/225

Abstract: A method of the present invention, for producing an iodizing agent, includes the step of electrolyzing iodine molecules in a solution by using an acid as a supporting electrolyte. This realizes (i) a method of producing an iodine cation suitable for use as an iodizing agent that does not require a sophisticated separation operation after iodizing reaction is completed, and (ii) an electrolyte used in the method. Further, a method of the present invention, for producing an aromatic iodine compound, includes the step of causing an iodizing agent, and an aromatic compound whose nucleus has one or more substituent groups and two or more hydrogen atoms, to react with each other under the presence of a certain ether compound. This realizes such a method of producing an aromatic iodine compound that position selectivity in iodizing reaction of an aromatic compound is improved.