公开(公告)号：US20160340719A1

公开(公告)日：2016-11-24

申请号：US15079322

申请日：2016-03-24

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: C12Q1/68 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6816 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N27/3278 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  G01N2015/0038 ,  Y10T29/49002 ,  Y10T29/49117 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.

公开(公告)号：US20160327507A1

公开(公告)日：2016-11-10

申请号：US15066117

申请日：2016-03-10

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH ,  Hui TIAN ,  Robert YUAN ,  John FOSTER ,  Roger CHEN

IPC: G01N27/327 ,  C12Q1/68 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

公开(公告)号：US20160216233A1

公开(公告)日：2016-07-28

申请号：US14606632

申请日：2015-01-27

Applicant: Genia Technologies, Inc.

Inventor： Jennifer Hovis ,  Hui Tian ,  Roger J.A. Chen

IPC: G01N27/447 ,  G01N33/487

CPC classification number: G01N33/48721

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

Abstract translation: 公开了一种纳米孔测序装置。 纳米孔测序装置包括工作电极。 还包括电介质层，其中电介质层的一部分水平地邻近工作电极设置，并且介电层的一部分设置在工作电极的一部分上方并覆盖其中，并且其中介电层形成具有 在工作电极的未覆盖部分上方的开口。 工作电极的基面面积大于工作电极的未覆盖部分上方开口的基面面积。

公开(公告)号：US09377437B2

公开(公告)日：2016-06-28

申请号：US14513642

申请日：2014-10-14

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Randy Davis

IPC: G01R27/28 ,  G01N27/447 ,  C12Q1/68 ,  G01N27/327 ,  B82Y30/00 ,  G01N15/00

CPC classification number: C12Q1/6816 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N27/3278 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  G01N2015/0038 ,  Y10T29/49002 ,  Y10T29/49117 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.

公开(公告)号：US20160178577A1

公开(公告)日：2016-06-23

申请号：US14577511

申请日：2014-12-19

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/447 ,  C12Q1/68 ,  B01L3/00

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

Abstract translation: 公开了分析纳米孔中分子的方法。 通过将纳米孔耦合到电压源，跨越插入膜中的纳米孔施加电压。 纳米孔与电压源分离。 在解耦之后，确定纳米孔上电压的衰减速率。 基于确定的纳米孔上的电压衰减速率，纳米孔中的分子与其它可能的分子区分开来。

公开(公告)号：US20160178576A1

公开(公告)日：2016-06-23

申请号：US14575938

申请日：2014-12-18

Applicant: Genia Technologies, Inc.

Inventor： Bill Maney ,  David Bruce Robinson ,  Markus Wallgren ,  Robert A. Yuan

IPC: G01N27/447 ,  G01N27/403

CPC classification number: G01N27/44791 ,  C12Q1/6869 ,  G01N27/403 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A nanopore based sequencing chip package is disclosed. The nanpore based sequencing chip package includes a reservoir defined by a plurality of surfaces. The chip package includes a nanopore cell array comprising a plurality of nanopore sensor cells enclosed by the reservoir. Each nanopore sensor cell has a working electrode. At least one surface of the reservoir is configured to be in contact with a conducting fluid when the conducting fluid is flowing through the reservoir. The chip package further includes a counter electrode disposed on the at least one surface of the reservoir.

Abstract translation: 公开了一种基于纳米孔的测序芯片封装。 基于纳基的测序芯片封装包括由多个表面限定的储存器。 芯片封装包括纳米孔单元阵列，其包括由储存器封闭的多个纳米孔传感器单元。 每个纳米孔传感器单元具有工作电极。 当导电流体流过储存器时，储存器的至少一个表面被配置为与导电流体接触。 芯片封装还包括设置在储存器的至少一个表面上的对电极。

公开(公告)号：US20160146365A1

公开(公告)日：2016-05-26

申请号：US14867918

申请日：2015-09-28

Applicant: Genia Technologies, Inc.

Inventor： Robert A. Yuan

IPC: F16K27/00

CPC classification number: F04B53/10 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/0663 ,  B01L2300/0627 ,  B01L2300/0867 ,  B01L2400/0644 ,  F16K27/003 ,  G01N33/48721

Abstract: A delivery system for a sensor chip includes a plurality of selectable ports arranged on a first assembly. Each of the selectable ports is in communication with a separate channel. The delivery system includes a second assembly movable in relation to the first assembly. The second assembly has a channel that is mechanically connectable to different ones of the plurality of selectable ports on the first assembly by motion of the second assembly relative to the first assembly. The delivery system includes a mechanical interface configured to engage a separate actuator so that relative motion of the first assembly and the second assembly is affected by the actuator.

Abstract translation: 用于传感器芯片的传送系统包括布置在第一组件上的多个可选择的端口。 每个可选择的端口与单独的通道通信。 输送系统包括可相对于第一组件移动的第二组件。 第二组件具有通过第二组件相对于第一组件的运动可机械地连接到第一组件上的多个可选择端口中的不同可选端口的通道。 输送系统包括被配置为接合单独的致动器的机械接口，使得第一组件和第二组件的相对运动受致动器的影响。

公开(公告)号：US20160027728A1

公开(公告)日：2016-01-28

申请号：US14808953

申请日：2015-07-24

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: H01L23/522 ,  G01N27/447 ,  H01L21/768 ,  H01L23/552 ,  H01L23/58

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

公开(公告)号：US20150337366A1

公开(公告)日：2015-11-26

申请号：US14376836

申请日：2013-02-15

Applicant: Genia Technologies, Inc.

Inventor： Randall DAVIS ,  Roger CHEN

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2320/32 ,  G01N33/48721 ,  G01N33/5432 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract translation: 本公开提供了用于制造生物芯片的生物芯片和方法。 生物芯片可以包括邻近或接近电极的膜（例如脂质双层）中的纳米孔。 描述了形成膜并将纳米孔插入膜中的方法。 生物芯片和方法可用于核酸（例如DNA）测序。 本公开还描述了使用生物芯片检测，分选和分类分子（例如蛋白质）的方法。

公开(公告)号：US20150211060A1

公开(公告)日：2015-07-30

申请号：US14591690

申请日：2015-01-07

Applicant: Genia Technologies, Inc.

Inventor： Kevin Deierling ,  Roger J.A. Chen ,  David J. Fullagar

IPC: C12Q1/68

CPC classification number: G01N27/4473 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/48721 ,  Y10T436/11 ,  C12Q2565/631 ,  C12Q2565/607

Abstract: A device for controlling, detecting, and measuring a molecular complex is disclosed. The device comprises a common electrode. The device further comprises a plurality of measurement cells. Each measurement cell includes a cell electrode and an integrator electronically coupled to the cell electrode. The integrator measures the current flowing between the common electrode and the cell electrode. The device further comprises a plurality of analog-to-digital converters, wherein an integrator from the plurality of measurement cells is electrically coupled to one analog-to-digital converter of the plurality of analog-to-digital converters.

Abstract translation: 公开了一种用于控制，检测和测量分子复合物的装置。 该装置包括公共电极。 该装置还包括多个测量单元。 每个测量单元包括电池耦合到电池电极的单元电极和积分器。 积分器测量在公共电极和电池电极之间流动的电流。 该装置还包括多个模数转换器，其中来自多个测量单元的积分器电耦合到多个模数转换器的一个模 - 数转换器。