公开(公告)号：US08268177B2

公开(公告)日：2012-09-18

申请号：US12673160

申请日：2008-08-13

Applicant: Jackie Y. Ying ,  Guolin Xu ,  Yoke San Daniel Lee

Inventor： Jackie Y. Ying ,  Guolin Xu ,  Yoke San Daniel Lee

IPC: B03C1/035 ,  B81B1/00

CPC classification number: B03C1/32 ,  B03C1/002 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/035 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/22 ,  B03C2201/26 ,  G01N35/0098

Abstract: A microfluidic separation system, which comprises a magnetic separator, which itself comprises a magnetic energy source; first and second magnetically conductive members leading from the magnetic energy source and having respective terminal ends that are separated by a gap over which a magnetic field is applied due to the magnetic energy source. The separation system further comprises a microfluidic chip for insertion into the gap, which comprises a body defining channels on respective faces of the body; and an exterior lining that seals the plurality of channels to allow separate test sample volumes to circulate in at least two of the channels. Upon insertion of the chip into the gap, a first test sample volume is confined to circulating closer to the terminal end of the first member and a second test sample volume is confined to circulating closer to the terminal end of the second member.

Abstract translation: 微流体分离系统，其包括磁分离器，其本身包括磁能源; 第一和第二导电构件，其从磁能源引出并且具有由由于磁能源而施加磁场的间隙分开的相应末端。 分离系统还包括用于插入到间隙中的微流体芯片，其包括在主体的相应面上限定通道的主体; 以及密封多个通道以允许单独的测试样品体积在至少两个通道中循环的外部衬里。 当将芯片插入到间隙中时，第一测试样本体积被限制为更靠近第一构件的终端循环，并且第二测试样本体积被限制为更靠近第二构件的终端循环。

公开(公告)号：US20120225473A1

公开(公告)日：2012-09-06

申请号：US13452054

申请日：2012-04-20

Applicant: Lotien Richard Huang ,  Thomas A. Barber ,  Bruce L. Carvalho ,  Ravi Kapur ,  Paul Vernucci ,  Mehmet Toner ,  Zihua Wang

Inventor： Lotien Richard Huang ,  Thomas A. Barber ,  Bruce L. Carvalho ,  Ravi Kapur ,  Paul Vernucci ,  Mehmet Toner ,  Zihua Wang

IPC: C12M1/00

CPC classification number: B03C1/30 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0472 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/32 ,  B03C2201/18 ,  B33Y80/00 ,  C12M47/04 ,  C12M47/06 ,  G01N1/40 ,  G01N1/4077 ,  G01N33/5044 ,  G01N2035/00237 ,  Y10T137/0318 ,  Y10T137/8593 ,  Y10T436/25 ,  Y10T436/25375

Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

Abstract translation: 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。

公开(公告)号：US08252187B2

公开(公告)日：2012-08-28

申请号：US12936087

申请日：2009-03-18

Applicant: Mukash Jassal ,  Neil Watson

Inventor： Mukash Jassal ,  Neil Watson

IPC: B01D35/06 ,  B01D21/26 ,  B03C1/02 ,  C02F1/48

CPC classification number: B04C9/00 ,  B01D21/0009 ,  B01D21/267 ,  B03C1/286 ,  B03C1/30 ,  B03C2201/18 ,  B04C5/13 ,  B04C2009/001 ,  C02F1/38 ,  C02F1/488 ,  C02F5/00 ,  C02F2303/08 ,  F24D19/0092

Abstract: A method and apparatus (10) for separating magnetic and non-magnetic particles from water in a domestic central heating system is disclosed. A magnet (60) is located in the housing and the inlet and the outlet are arranged so that, in use, water flows through the apparatus in a cyclonic motion from the inlet downwardly proximate the walls of the housing, and then upwardly within the downwardly flowing water, to the outlet. Particles entrained within the water separate out by vortex separation as the water flows downwardly. Also, magnetic particles entrained within the water are collected on the magnet as the water flows upwardly. The apparatus also comprises a sleeve (30) located within the housing such that an outer circulation channel (31) is defined between the housing and the sleeve. The magnet is located within the sleeve such that an inner circulation channel (61) is defined between the sleeve and the magnet. The inlet (40) of the housing is arranged to deliver the water into the outer circulation channel, and the outlet (50) of the housing is arranged to exhaust the water from the inner circulation channel.

Abstract translation: 公开了一种用于在家用中央供暖系统中从水中分离磁性和非磁性颗粒的方法和装置（10）。 磁体（60）位于壳体中，并且入口和出口被布置成使得在使用中，水以气旋的方式从入口向下靠近壳体的壁部从气流中流过设备，然后在向下 流水，到出口。 随着水向下流动，夹带在水中的颗粒通过涡流分离分离出来。 此外，随着水向上流动，夹带在水中的磁性颗粒被收集在磁体上。 设备还包括位于壳体内的套筒（30），使得在壳体和套筒之间限定外部循环通道（31）。 磁体位于套筒内，使得内部循环通道（61）被限定在套筒和磁体之间。 壳体的入口（40）布置成将水输送到外循环通道中，并且壳体的出口（50）布置成从内循环通道排出水。

公开(公告)号：US20120211428A1

公开(公告)日：2012-08-23

申请号：US13369338

申请日：2012-02-09

Applicant: Markus Zahn ,  T. Alan Hatton ,  Shahriar Rohinton Khushrushahi

Inventor： Markus Zahn ,  T. Alan Hatton ,  Shahriar Rohinton Khushrushahi

IPC: B03C1/02 ,  B03C1/01 ,  C02F1/48

CPC classification number: C02F1/488 ,  B01D17/047 ,  B01D17/06 ,  B03C1/01 ,  B03C1/02 ,  B03C11/00 ,  B03C2201/18 ,  C02F1/281 ,  C02F1/681 ,  Y10S210/925

Abstract: Method for oil removal. The method includes adding a magnetizable material, with or without appropriately selected surfactants, of order micron (having no net magnetization) or nanometer size to magnetize the oil or water phase by either making a ferrofluid, magnetorheological fluid, a magnetic Pickering emulsion (oil in water or water in oil emulsion), or any other process to magnetize either oil or water phases. The magnetized fluid is separated from the non-magnetic phase using novel or existing magnetic separation techniques or by permanent magnets or electromagnets thereby separating oil and water phases. The magnetized particles are separated from the magnetized phase using novel or existing magnetic separation techniques to recover and reuse the particles. The two magnetic separation steps can be repeated to further increase recovery efficiency of the liquid phases and the magnetizable particles reused in this continuous process.

Abstract translation: 除油方法 该方法包括添加具有或不具有适当选择的表面活性剂的可磁化材料，其具有微米级（无净磁化强度）或纳米尺寸以通过制备铁磁流体，磁流变流体，磁性Pickering乳液（在 水或油包水乳液）或任何其他磁化油相或水相的过程。 使用新的或现有的磁分离技术或永久磁铁或电磁铁将磁化流体与非磁性相分离，从而分离油相和水相。 使用新的或现有的磁分离技术将磁化的颗粒与磁化相分离以回收和再利用颗粒。 可以重复两个磁选分离步骤，以进一步提高在该连续过程中重新使用的液相和可磁化颗粒的回收效率。

公开(公告)号：US08216454B2

公开(公告)日：2012-07-10

申请号：US11997390

申请日：2006-08-09

Applicant: Hideo Araseki

Inventor： Hideo Araseki

IPC: C02F1/48 ,  B01D35/06

CPC classification number: B03C1/288 ,  B03C2201/18 ,  C22B9/003 ,  C22B9/02 ,  C22B21/0092 ,  C22B21/06 ,  Y02P10/218 ,  Y02P10/234 ,  Y02P10/253

Abstract: An apparatus comprising at least vessel (2) for stocking conductive liquid (1) containing impurities; electromagnetic force generator (3) for generating electromagnetic force (F) capable of circulating the conductive liquid (1) in the conductive liquid (1); and discharging unit (4) for discharging nonmetallic impurities and deemed nonmetallic impurities (21a,21b) accumulated in low-pressure region (1a) by a pressure difference in conductive liquid (1) induced by the electromagnetic force (F), so that by the electromagnetic force (F), any nonmetallic impurities (21a) and deemed nonmetallic impurities (21b) are driven to the low-pressure region (1a), for example, liquid surface and separated.

Abstract translation: 一种装置，至少包括容纳包含杂质的导电液体（1）的容器（2） 用于产生能够使导电液体（1）在导电液体（1）中循环的电磁力（F）的电磁力发生器（3）; 和排出单元（4），用于通过由电磁力（F）引起的导电液体（1）中的压力差来排放积聚在低压区域（1a）中的非金属杂质和非金属杂质（21a，21b），从而通过 电磁力（F），任何非金属杂质（21a）和被认为非金属杂质（21b）被驱动到低压区域（1a），例如液面并分离。

公开(公告)号：US08206596B2

公开(公告)日：2012-06-26

申请号：US12224030

申请日：2008-02-22

Applicant: Nongyue Wang ,  Xiaogen Feng ,  Xiaohui Mao

Inventor： Nongyue Wang ,  Xiaogen Feng ,  Xiaohui Mao

IPC: B03C1/02

CPC classification number: B03C1/12 ,  B03C1/0332 ,  B03C1/286 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/28

Abstract: The present invention relates to a magnetic separation apparatus for continuous separating and recovering magnetic solid particles from a solid-liquid mixture. The apparatus includes at least one magnetic separation unit and each unit includes: an outer cylindrical vessel having a material inlet, a first outlet, and a second outlet; an inner cylindrical vessel, at least part of which extends along the axis inside the first cylindrical vessel without contacting with the inner surface of the outer cylindrical vessel; and a magnet, rendering the bottom of the inner cylindrical vessel magnetism during the first period and making the part of the surface lose its magnetism during a second period. When the solid-liquid mixture flows through the magnetic surface of the inner cylindrical vessel in the passage, the magnetic solids are absorbed and separated from the mixture.

Abstract translation: 本发明涉及一种用于从固液混合物中连续分离和回收磁性固体颗粒的磁选机。 该装置包括至少一个磁选机，每个单元包括：具有材料入口，第一出口和第二出口的外圆柱形容器; 内圆柱形容器，其至少一部分沿第一圆柱形容器内的轴线延伸，而不与外圆柱形容器的内表面接触; 和一个磁体，使第一个时间段内部圆柱形磁体的磁场的底部，并使表面的一部分在第二个时间段内失去磁性。 当固体 - 液体混合物流过通道内圆柱形容器的磁性表面时，磁性固体被吸收并与混合物分离。

公开(公告)号：US20120145641A1

公开(公告)日：2012-06-14

申请号：US13217244

申请日：2011-08-25

Applicant: Justin Rebo ,  Ben Zealley ,  John Schloendorn ,  Mark Hamalainen ,  Keith Causey

Inventor： Justin Rebo ,  Ben Zealley ,  John Schloendorn ,  Mark Hamalainen ,  Keith Causey

IPC: B03C1/02

CPC classification number: B03C1/01 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26

Abstract: A major type of unwanted cells that accumulate in aging are anergic cytotoxic T-cells. These cells often have virus-specific T-cell receptors, as well as other surface markers that distinguish them from their youthful counterparts, and are thought to play a major role in the decline of the immune system with age. Here we disclose the use of magnetic cell separation methods and apparatuses to remove senescent T-cells defined by the surface markers CD8+, KLRG1+ and CD8+, CD28−. We disclose how these markers are used to remove anergic T-cells from the blood of aged C57BL/6 mice, resulting in lasting rejuvenation of their immunological marker profile. Using antibodies with magnetic microparticles linked to their Fc domains, we first developed a method to use magnets to filter out the unwanted cells from the blood, and later constructed a device that does this automatically.

Abstract translation: 在老化中积累的主要类型的不需要的细胞是无菌细胞毒性T细胞。 这些细胞通常具有病毒特异性T细胞受体，以及将其与年轻对应物区分开的其他表面标志物，并被认为在免疫系统随年龄的衰退中起重要作用。 在这里，我们公开了使用磁细胞分离方法和装置来去除由表面标志物CD8 +，KLRG1 +和CD8 +，CD28-定义的衰老T细胞。 我们公开了如何使用这些标记物从老年C57BL / 6小鼠的血液中除去无能T细胞，导致其免疫学标志物谱的持久复兴。 使用具有与其Fc结构域连接的磁性微粒的抗体，我们首先开发了一种使用磁体从血液中滤出不想要的细胞的方法，并且随后构建了自动完成的装置。

公开(公告)号：US20120128550A1

公开(公告)日：2012-05-24

申请号：US13300347

申请日：2011-11-18

Applicant: Matti KORPELA ,  Kenneth RUNDT

Inventor： Matti KORPELA ,  Kenneth RUNDT

IPC: B01D11/02

CPC classification number: G01N1/28 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/26 ,  G01N33/54326 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Method for handling microparticles in such a manner, that at least two treatment steps are performed for microparticles in the same vessel without moving the particles to another vessel. There are organs in the device for changing the solution without having to move the microparticles to another vessel.

Abstract translation: 以这样的方式处理微粒的方法，即对同一容器中的微粒进行至少两个处理步骤，而不将颗粒移动到另一个容器。 在用于改变溶液的装置中存在器官，而不必将微粒移动到另一个容器。

公开(公告)号：US20120103815A1

公开(公告)日：2012-05-03

申请号：US13317588

申请日：2011-10-21

Applicant: Guy L. McClung, III

Inventor： Guy L. McClung, III

IPC: C02F1/48 ,  B03C5/02 ,  B82Y30/00 ,  B82Y99/00

CPC classification number: B01D43/00 ,  A61M1/3618 ,  A61M2205/0272 ,  B01D21/262 ,  B03C1/01 ,  B03C1/02 ,  B03C5/00 ,  B03C2201/18 ,  B03C2201/20 ,  B82Y30/00 ,  C02F1/02 ,  C02F1/48 ,  C02F1/481 ,  C02F1/484 ,  C02F2103/08 ,  C02F2103/10 ,  C02F2209/02 ,  C02F2303/04 ,  C02F2305/08 ,  C25D15/00

Abstract: Systems and methods for treating a fluid by passing fluid through a treatment structure, the fluid containing undesirable living things, the treatment structure containing electrically conductive nanomaterial with silver, flowing an electric current in the fluid in the treatment structure via the electrically conductive nanomaterial with silver or silver material to kill undesirable living things in the treatment structure, and killing undesirable things in the treatment structure.

Abstract translation: 通过使流体通过处理结构来处理流体的系统和方法，所述流体含有不期望的生物，所述处理结构含有银的导电纳米材料，通过所述导电纳米材料与所述导电纳米材料在银处理结构中流动电流 或银料，以杀死治疗结构中的不良生物，并在治疗结构中杀死不良物质。

公开(公告)号：US20120085684A1

公开(公告)日：2012-04-12

申请号：US13268146

申请日：2011-10-07

Applicant: Peter Carlo Rem ,  Simon Peter Maria Berkhout

Inventor： Peter Carlo Rem ,  Simon Peter Maria Berkhout

IPC: B03C1/30 ,  B03B5/68 ,  B03C1/16 ,  B07C5/00

CPC classification number: B03C1/22 ,  B03C2201/18 ,  C22B1/005 ,  C22B7/005 ,  Y02P10/212

Abstract: Method for separating a non-ferrous metal-comprising fraction from ferrous scrap, wherein the ferrous scrap is conveyed to a processing device for separating the non-ferrous metal-comprising fraction from the remainder of the ferrous scrap. The processing device supplies a beam of water, and the ferrous scrap is conveyed and released into the waterbeam so as to cause that the ferrous scrap is allowed to fall and move further, subject to the forces of gravity and the waterbeam.

Abstract translation: 从含铁金属废料中分离有色金属部分的方法，其中将废铁转移到处理装置中，用于将含铁金属的部分与剩余的含铁废料分离。 处理装置供应一束水，并将亚铁废料输送并释放到水束中，以使得废铁被允许在重力和水束的作用下进一步下落和移动。