Abstract:
A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three- dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.
Abstract:
Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent.
Abstract:
Screens generated by a host application are reformatted for viewing by applying styles to the host screens in response to recognized components included therein. In particular, a style defines a desired look and layout to be applied to a respective host screen. The styles are applied to the respective host screens based on recognizing components of the host screen at run time. For example, first and second styles can be associated with respective first and second components included in host screens. The first style is applied to the host screen in which the first component is recognized and the second style is applied to the host screen in which the second component is recognized. Accordingly, the application of the first and second styles provide respective first and second reformatted screens.
Abstract:
A brazed leaded package is provided having buried brazed areas thereby eliminating the need of subsequent protective plating of the brazed areas. The package has a heat conductive support which supports a plurality of leads. The distal end of the leads terminate on the top of the heat conductive support in a braze pad to which are brazed formed leads for providing interface connection to a semiconductor integrated circuit contained within the package. The package can be sealed by using a solid ceramic cover having a glazed glass on its bottom side which is heated up to reflow and provide the seal.
Abstract:
A device for controlling buoyancy of a buried pipeline, the device comprising a form placed atop the pipeline and a flexible strap extending around the form adjacent its top. The form may comprise a first trough located adjacent the first side of the pipeline, a second trough located adjacent the opposing second side of the pipeline, and a middle section with an inverted-U-shaped cross section such that the middle portion fits over the top of the pipeline and joins the first trough and the second trough. The troughs are filled with backfill when the pipeline is buried. Thus, when fully installed, the device may comprise the slot form, the strap, and the backfill. As the pipeline tends to rise, the straps deform, bringing the sides of the troughs inward and compressing the backfill therein. This prevents further upward movement of the pipeline.
Abstract:
The present invention provides a method for the detection of a material comprising ammonium nitrate and a sugar, the method comprising sensing for the presence of hydrogen isocyanate (HNCO). The present invention further provides an apparatus suitable for the examination of a material suspected of comprising ammonium nitrate and a sugar, the apparatus comprising heating means in thermal communication with a sample holder suitable for the containment of the material, the sample holder being in gaseous communication with sensing region, the apparatus being further provided with a means for inducing flow of gas from the sample holder to the sensing region and a means for sensing the presence of hydrogen isocyanate in the sensing region.
Abstract:
A thematic microarray and methods for multiplexed binding assays using a cocktail solution of labeled ligands in the presence or absence of a target compound is provided. The methods enables researchers to screening compounds against multiple targets using a microarray format.
Abstract:
A connector affixed to the end of a cable of optical fibers and having alignment pins extending axially in parallel with the cable and beyond the end thereof. A substrate with alignment holes and light transmitting openings therethrough in axial alignment with the ends of the optical fibers when the alignment pins and holes are engaged. The substrate including mounting pads, electrical connection pins and electrical traces connecting the pads to the pins. A semiconductor die, with photonic devices thereon, mounted on the mounting pads of the substrate so that each photonic device is aligned with a light transmitting opening and electrically connected to the electrical connection pins. A sleeve surrounding and securing the connector to the mounting member.
Abstract:
Cell-free extracts, methods for producing these extracts, methods for using these extracts, compositions that facilitate production of these extracts and kits that contain these extracts are provided. By increasing or decreasing certain gene products through, for example, the use of siRNA or mimics, one can develop mammalian cell-free extracts that have desired levels of efficiency.
Abstract:
A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.