公开(公告)号：US20230204495A1

公开(公告)日：2023-06-29

申请号：US17996581

申请日：2021-04-27

Applicant: CYTIVA SWEDEN AB

Inventor： Hanno Ehring ,  David Gronowitz ,  Lars Rosengren

IPC: G01N21/27 ,  G01N21/31

CPC classification number: G01N21/274 ,  G01N21/31

Abstract: The present invention relates to computer implemented method performed by a computer (110) configured to calibrate an apparatus for measuring the absorbance of light of a substance (131) for a chromatography system (100), the apparatus (131) comprising a conduit (C) for enabling a fluid to be measured, a light emitter (LE) configured to emit light along an optical path (OP) to a light sensor (S) configured to measure the emitted light, the optical path intersecting the conduit (C), a rotating disc (D) having one or more optical filters, each of the one or more optical filters is arranged with its center passing through the optical path (OP) when rotating, an actuator configured to rotate the disc (D) dependent on a control signal, to the method comprising controlling rotation of (710) the disc (D) to a first position where a first filter, of the one or more optical filters, intersects the optical path (OP) at a first point (P1), measuring (720) a first light absorption value, controlling rotation of (730) the disc (D) to a second position, different to the first position, where the first filter still intersects the optical path (OP) at a second point (P2), measuring (740) a second light absorption value, generating (750) an aggregated light absorption value, calibrating (760) the apparatus (131) by comparing the aggregated light absorption value to a reference light absorption value.

公开(公告)号：US20230159958A1

公开(公告)日：2023-05-25

申请号：US17995571

申请日：2021-04-06

Applicant: CYTIVA SWEDEN AB

Inventor： Andreas Jonsson ,  Daniel Ivansson

IPC: C12N15/90 ,  C12N9/12 ,  C12N15/85

CPC classification number: C12N15/907 ,  C12N9/12 ,  C12N15/85 ,  C12N2800/30 ,  C12N2800/106

Abstract: The present disclosure relates to a method for targeted integration of a donor vector into a specific pre-defined genomic location of an isolated eukaryotic host cell. The vector and host cell together comprise nucleic acid components allowing for the selection of cells having integrated the donor vector into the pre-defined genomic location of the host cell. In addition, it provides for the identification of any random integrations of the donor vector(s) into other parts of the host cell genome. Once identified, such cells present an excellent alternative for subsequent recombinant protein production.

公开(公告)号：US11648490B2

公开(公告)日：2023-05-16

申请号：US17113311

申请日：2020-12-07

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Bjorn Markus Olovsson

IPC: B01D15/18 ,  G01N30/20 ,  G01N30/42 ,  G01N30/46 ,  G01N30/88

CPC classification number: B01D15/1842 ,  G01N30/20 ,  G01N30/461 ,  G01N30/466 ,  G01N30/467 ,  G01N30/88 ,  G01N30/42 ,  G01N30/468 ,  G01N2030/205 ,  G01N2030/8881

Abstract: Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

公开(公告)号：US20230068163A1

公开(公告)日：2023-03-02

申请号：US17938303

申请日：2022-10-05

Applicant: CYTIVA SWEDEN AB

Inventor： Nils Stafstrom ,  Bjorn Johansson ,  Andreas Marcstrom

IPC: B01D19/00 ,  B01D19/02 ,  B05B1/06 ,  B05B1/08

Abstract: The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping device configured to remove air from a fluid and comprising the nozzle.

公开(公告)号：US11571635B2

公开(公告)日：2023-02-07

申请号：US15562586

申请日：2016-03-23

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson ,  Anders Ljunglof ,  Karol Maciej Lacki

IPC: B01D15/00 ,  B01D15/38 ,  B01D15/18 ,  B01D15/14 ,  G01N30/46 ,  C07K1/22 ,  C12N7/00 ,  G01N30/20 ,  G01N30/88

Abstract: The present invention relates to a liquid chromatography system for the separation of bio-molecules in a fluid including at least two unit operations, wherein the first unit operation is a step of multi-column chromatography and the second unit operation is a step modifying said bio-molecules and/or the fluid, wherein the modification comprises feeding the fluid resulting from the last chromatography column of the first unit operation into a system comprising at least two containers, wherein each container has a volume and a moveable sidewall arranged to divide the volume into a first sub-volume and a second sub-volume, and each container comprises a first port connected to the first volume and a second port connected to the second sub-volume. The invention also relates to a virus inactivation device for a chromatography system according to the invention, which enables continuous or semi-continuous processing of biomolecules, as well as a method of using such a device.

公开(公告)号：US20230014015A1

公开(公告)日：2023-01-19

申请号：US17777590

申请日：2020-12-11

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer

IPC: G05D11/13 ,  G01N30/34

Abstract: A bioprocess fluid mixing system (3; 3′; 3″; 103; 103′), said fluid mixing system (3; 3′; 3″; 103; 103′) comprising: —at least two fluid inlets (5a, 5b, 5c, 5d, 5e), configured for providing a first fluid into the fluid mixing system through a first fluid inlet (5a) and for providing a second fluid into the fluid mixing system through a second fluid inlet (5b); —at least one valve arrangement (13a, 13b, 13c, 13a′), where a first valve arrangement (13a; 13a′) is in fluid communication with at least both the first fluid inlet (5a) and the second fluid inlet (5b); —at least two pumps (11a, 11b, 11c, 11d, 11e), where a first pump (11a) is in selective fluid communication with at least both the first and the second fluid inlets (5a, 5b) via the first valve arrangement (13a; 13a′) and a second pump (11b) is in fluid communication with at least one of the first and second fluid inlet (5b); and —a common fluid outlet (14) which is in fluid communication with both an outlet (15a) of the first pump (11a) and an outlet (15b) of the second pump (11b), wherein pump rates of the at least two pumps (11a, 11b) and valve positions in the at least one valve arrangement (13a; 13a′; 13b, 13c) are configured to be controllable by a control system (21) such that mixing of at least a first fluid from the first fluid inlet (5a) and a second fluid from the second fluid inlet (5b) can be performed to a requested mixing of the at least two fluids and to a requested combined fluid flow rate at the common fluid outlet (14).

公开(公告)号：US11547956B2

公开(公告)日：2023-01-10

申请号：US16622524

申请日：2018-06-28

Applicant: Cytiva Sweden AB

Inventor： Mats Lundkvist ,  Bjorn A. Johansson ,  Tim Francois

IPC: B01D15/22 ,  F16L37/138 ,  F16L37/56 ,  G01N30/60 ,  G01N30/02

Abstract: Disclosed are chromatography systems employing a releasable coupling (100) for holding a fluid tubing to a spigot, the coupling comprising: a cylindrical inner component (110) for accepting a fluid tubing (30), said inner component including a resiliently deflectable portion (118) arranged to urge an outer surface of the tubing toward a spigot; and a cylindrical collar (130) having and internal through-aperture (132) for slideably accepting the inner component, the aperture and resilient portion having complementary surface formations which in a first position of the collar mounted to the inner component provide for said resilient deflection in use, and which in a second different position do not cause said deflection, the coupling being characterized in that the collar comprises a collar flange (134) extending outwardly away from the aperture of a size allowing manual manipulation of the collar between the first and second positions. Chromatography systems employing said couplings is disclosed also.

公开(公告)号：US11493489B2

公开(公告)日：2022-11-08

申请号：US16975324

申请日：2019-02-20

Applicant: Cytiva Sweden AB

Inventor： Jens Widehammar ,  Key Hyckenberg ,  Orjan Grelsson ,  Lars Mattsson ,  Kjell Karlsson

IPC: G01N30/86 ,  G01N30/02

Abstract: The present invention relates to a liquid chromatography system (90) configured to operate with at least one column and configured for purification of a sample comprising a target product using a predefined process. The liquid chromatography system comprises a controller (91) configured to: control the operation of the chromatography system to run the predefined process; retrieve column data accessible from a data storage, the column data being specific to each column; and adapt at least one process parameter of the predefined process for each column based on column data. Whereby the predefined process is adapted to each column to obtain the target product and maintain the performance of the liquid chromatography system.

公开(公告)号：US20220336042A1

公开(公告)日：2022-10-20

申请号：US17640771

申请日：2020-09-29

Applicant: Cytiva Sweden AB

Inventor： Tobias Soderman ,  Olof Karlsson ,  Magnus Raberg ,  Andreas Borgstrom

IPC: G16B15/00 ,  G16B40/20 ,  G01N21/552 ,  G01N21/27 ,  G06N3/08

Abstract: Disclosed is a method for classifying monitoring results from an analytical sensor system (20) arranged to monitor molecular interactions at a sensing surface, wherein detection curves representing progress of the molecular interactions with time are produced. The method comprises steps of: acquiring (100) a set of detection curves, fitting (101) a first mathemati- cal model to the set of detection curves; calculating (102) a set of features from the set of detection curves and fitted mathematical model; based on the calculated set of features, classifying (103) each detection curve into qual- ity classification group; and based on the classification determining which detection curves to use in kinetic analysis of the monitored molecular inter- actions.

公开(公告)号：US20220336041A1

公开(公告)日：2022-10-20

申请号：US17636288

申请日：2020-09-08

Applicant: Cytiva Sweden AB

Inventor： Fredrik Edebratt ,  Olof Karlsson ,  Anna Lager ,  Jens Larsson

IPC: G16B15/00 ,  G01N33/68 ,  G16B40/20 ,  G16B45/00

Abstract: Disclosed is a method of qualifying a subgroup of target binding biomolecules from a larger group of target binding biomolecules for analysis. A competitive immunoassay including a target protein is used to identify 100 interactions between different pairs of the target binding biomolecules and interaction profiles are generated 200. Each target binding biomolecule is allocated 300 to a bin representing an epitope family and identified bins are associated in a circular or semi-circular bin chart on a display with identified respective target binding biomolecule(s). Based on the association 400 between identified bins and identified respective target binding molecule(s) in the bin chart, a subgroup of target binding biomolecules is selected 500 for further analysis by selecting one or more of the target binding biomolecule(s) of one or more of the bins.