公开(公告)号：US20180306181A1

公开(公告)日：2018-10-25

申请号：US15955552

申请日：2018-04-17

Applicant: Genia Technologies, Inc.

Inventor： Robert A. Yuan

IPC: F04B53/10

CPC classification number: F04B53/10 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/0663 ,  B01L2300/0627 ,  B01L2300/0867 ,  B01L2400/0644 ,  F16K27/003 ,  G01N33/48721

Abstract: A delivery system for a sensor chip includes a plurality of selectable ports and a two-way pump port selectively connectable to each of the selectable ports. The two-way pump port is configured to allow material to be drawn or delivered from or to the two-way pump port. The delivery system also includes a chamber and a bypass waste channel that is selectively connectable to the two-way pump port. The plurality of selectable ports includes a selectable chamber port connected to the chamber and the chamber has a chamber waste exit. Material may selectively flow through the chamber to a waste collection via the chamber waste exit or flow to the waste collection via the bypass waste channel that bypasses the chamber waste exit.

公开(公告)号：US10036739B2

公开(公告)日：2018-07-31

申请号：US14606632

申请日：2015-01-27

Applicant: Genia Technologies, Inc.

Inventor： Jennifer Hovis ,  Hui Tian ,  Roger J. A. Chen

IPC: G01N27/447 ,  G01N33/487

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

公开(公告)号：US09970437B2

公开(公告)日：2018-05-15

申请号：US14867922

申请日：2015-09-28

Applicant: Genia Technologies, Inc.

Inventor： Robert A. Yuan

IPC: B01L3/02 ,  B01L3/00 ,  G01N1/10 ,  F04B53/10 ,  F16K27/00 ,  G01N33/487

CPC classification number: F04B53/10 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/0663 ,  B01L2300/0627 ,  B01L2300/0867 ,  B01L2400/0644 ,  F16K27/003 ,  G01N33/48721

Abstract: A delivery system for a sensor chip includes a plurality of selectable ports and a two-way pump port selectively connectable to each of the selectable ports. The two-way pump port is configured to allow material to be drawn or delivered from or to the two-way pump port. The delivery system also includes a chamber and a bypass waste channel that is selectively connectable to the two-way pump port. The plurality of selectable ports includes a selectable chamber port connected to the chamber and the chamber has a chamber waste exit. Material may selectively flow through the chamber to a waste collection via the chamber waste exit or flow to the waste collection via the bypass waste channel that bypasses the chamber waste exit.

公开(公告)号：US09863904B2

公开(公告)日：2018-01-09

申请号：US14971667

申请日：2015-12-16

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/447 ,  G01N27/22 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G01N27/228 ,  C12Q1/6869 ,  G01N27/3273 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

公开(公告)号：US20170322195A1

公开(公告)日：2017-11-09

申请号：US15488432

申请日：2017-04-14

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: G01N33/487 ,  B81B1/00 ,  G01N15/12 ,  B82Y5/00

CPC classification number: G01N33/48721 ,  B81B1/00 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N15/12 ,  G01N15/1218

Abstract: A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

公开(公告)号：US20170203520A1

公开(公告)日：2017-07-20

申请号：US15411780

申请日：2017-01-20

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: B29C67/20 ,  G01N33/487 ,  C12Q1/68

CPC classification number: B29C67/20 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q2565/631

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US20170159117A1

公开(公告)日：2017-06-08

申请号：US15435206

申请日：2017-02-16

Applicant: Genia Technologies, Inc.

Inventor： Randall W Davis ,  Roger J.A. Chen

IPC: C12Q1/68 ,  G01N33/487 ,  G01N27/447 ,  B82Y15/00

CPC classification number: C12Q1/6869 ,  B82Y10/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N27/447 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2525/119 ,  C12Q2525/121 ,  C12Q2525/204 ,  C12Q2527/101 ,  C12Q2563/149 ,  C12Q2565/631

Abstract: The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.

公开(公告)号：US09658190B2

公开(公告)日：2017-05-23

申请号：US14575938

申请日：2014-12-18

Applicant: Genia Technologies, Inc.

Inventor： Bill Maney ,  David Bruce Robinson ,  Markus Wallgren ,  Robert A. Yuan

IPC: G01N27/447 ,  G01N27/403 ,  G01N33/487 ,  C12Q1/68

CPC classification number: G01N27/44791 ,  C12Q1/6869 ,  G01N27/403 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A nanopore based sequencing chip package is disclosed. The nanopore based sequencing chip package includes a reservoir defined by a plurality of surfaces. The chip package includes a nanopore cell array comprising a plurality of nanopore sensor cells enclosed by the reservoir. Each nanopore sensor cell has a working electrode. At least one surface of the reservoir is configured to be in contact with a conducting fluid when the conducting fluid is flowing through the reservoir. The chip package further includes a counter electrode disposed on the at least one surface of the reservoir.

公开(公告)号：US20160001250A1

公开(公告)日：2016-01-07

申请号：US14794524

申请日：2015-07-08

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.

Abstract translation: 公开了一种用于调节测量阵列的温度的系统。 该系统包括包括多个传感器的测量阵列，其中多个传感器集成在集成电路管芯上。 该系统包括集成在集成电路管芯上的热传感器，其中热传感器感测与多个传感器相关联的温度。 该系统还包括耦合到集成电路管芯的热泵，其中热泵由包括热传感器的反馈控制电路控制。

公开(公告)号：US20150152494A1

公开(公告)日：2015-06-04

申请号：US14521425

申请日：2014-10-22

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH

IPC: C12Q1/68 ,  G01N27/327

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

Abstract translation: 本公开提供了包括多个孔的生物芯片。 生物芯片包括设置在多个孔的单个井中或邻近多个井的单个井的膜。 膜包括纳米孔，并且单个孔包括电极，其响应于通过或邻近纳米孔的物质而在离子流动通过孔时检测信号。 电极可以是非牺牲电极。 可以使用气泡在多个孔上形成脂质双层。