公开(公告)号：US11708592B2

公开(公告)日：2023-07-25

申请号：US17120005

申请日：2020-12-11

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya ,  Mark J. Burk

IPC: C12P17/08 ,  C12N15/70 ,  C08G63/08 ,  C07D313/04 ,  C12N15/63 ,  C12P17/02 ,  C08G18/42 ,  C12N5/00 ,  C12N15/52

CPC classification number: C12P17/08 ,  C07D313/04 ,  C08G18/4277 ,  C08G63/08 ,  C12N5/0018 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12P17/02

Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.

公开(公告)号：US11685908B2

公开(公告)日：2023-06-27

申请号：US16978629

申请日：2019-03-08

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Kevin G. Hoff ,  Anna Lechner ,  Harish Nagarajan

IPC: C12N9/10 ,  C12P7/22 ,  C12P7/42

CPC classification number: C12N9/1085 ,  C12P7/22 ,  C12P7/42

Abstract: Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control. Prenyltransferase variants also demonstrated regioselectivity to desired cannabinoid isomers such as CDBA (3-GOLA), 3-GDVA, 3-GOSA, and CBG (2-GOL). The prenyltransferase variants can be used to form prenylated aromatic compounds, and can be expressed in an engineered microbe having a pathway to such compounds, which include 3-GOLA, 3-GDVA, 3-GOSA, and CBG. 3-GOLA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

公开(公告)号：US20230065295A1

公开(公告)日：2023-03-02

申请号：US17369871

申请日：2021-07-07

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Stephen J. Van Dien ,  Robin E. Osterhout ,  Mark J. Burk ,  John D. Trawick ,  Michael P. Kuchinskas ,  Brian Steer

IPC: C12N1/20 ,  C12P7/18 ,  C12N9/18 ,  C12N15/52 ,  C12P7/42 ,  C12N1/14 ,  C12N1/16 ,  C12N15/63 ,  C07C31/20 ,  C07C59/01 ,  C08G18/28 ,  C08G18/32 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.

公开(公告)号：US11525149B2

公开(公告)日：2022-12-13

申请号：US15600479

申请日：2017-05-19

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

IPC: C12P7/46 ,  C12N15/00 ,  C12N9/00 ,  C12P7/04 ,  C12P7/40 ,  C12N9/88

Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

公开(公告)号：US20220333143A1

公开(公告)日：2022-10-20

申请号：US17526632

申请日：2021-11-15

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard

IPC: C12P7/64 ,  C12P7/6409 ,  C12P7/04 ,  C12P7/24 ,  C12N15/52 ,  C07C31/20 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/88 ,  C12N9/00

Abstract: The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.

公开(公告)号：US11371063B2

公开(公告)日：2022-06-28

申请号：US16664549

申请日：2019-10-25

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Mark J. Burk

IPC: C12P5/02 ,  C12N15/52 ,  C12P3/00 ,  C12P7/40 ,  C01B3/00 ,  C08F36/06 ,  C08F36/14 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12

Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.

公开(公告)号：US11371046B2

公开(公告)日：2022-06-28

申请号：US15270578

申请日：2016-09-20

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

IPC: C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12P7/52 ,  C12P17/04 ,  B01D3/00 ,  C12N15/70 ,  C12N15/81

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

公开(公告)号：US11359216B2

公开(公告)日：2022-06-14

申请号：US16889211

申请日：2020-06-01

Applicant: Genomatica, Inc.

Inventor： Andreas W. Schirmer ,  Noah Helman ,  Haibo Wang ,  Zhihao Hu ,  Vikranth Arlagadda ,  Alma Itzel Ramos-Solis ,  Elizabeth Clarke

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52

Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US11339384B2

公开(公告)日：2022-05-24

申请号：US16500188

申请日：2018-04-03

Applicant: Genomatica, Inc.

Inventor： Angelica Zabala Bautista ,  Min Sun Park ,  Alma Itzel Ramos-Solis ,  Bernardo Moura Torres Da Costa ,  Behnaz Behrouzian ,  Donald E. Trimbur ,  Emanuela Popova ,  Qijun Hu ,  Stephen Bralley Del Cardayre

IPC: C12N9/16 ,  C12P7/6409

Abstract: The disclosure relates to engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives including e.g., eight carbon and ten carbon fatty acids and fatty acid derivatives. The disclosure further relates to recombinant host cells comprising the engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives. The disclosure also relates to methods of decreasing toxicity and improving production of medium-chain fatty acids and derivatives.

公开(公告)号：US11293026B2

公开(公告)日：2022-04-05

申请号：US16550010

申请日：2019-08-23

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

IPC: C12N1/21 ,  C12P7/44 ,  C12N9/04 ,  C12N9/12 ,  C12N9/10 ,  C12N9/88 ,  C12N9/02 ,  C12N9/16 ,  C12N15/52 ,  C12P13/00 ,  C12P13/02 ,  C12P17/10 ,  C12N9/80 ,  C12P7/46 ,  C12P7/62

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.