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18 条记录 (耗时 0.018 秒)

    Method for producing secretable glycosyltransferases and other Golgi processing enzymes
    14.
    发明授权
    Method for producing secretable glycosyltransferases and other Golgi processing enzymes 失效
    生产可分泌糖基转移酶和其他高尔基体加工酶的方法

    公开(公告)号：US5032519A

    公开(公告)日：1991-07-16

    申请号：US426577

    申请日：1989-10-24

    申请人： James C. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne

    发明人： James C. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne

    IPC分类号： C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12P19/18 ,  C12P19/26 ,  C12R1/91

    CPC分类号： C12N9/1051 ,  C12P19/18 ,  C12P19/26

    摘要： A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a rThis invention was made with government support under Grant Contract Nos. GM-27904 and GM-11557 awarded by the National Institute of Health. The government has certain rights in this invention. The publications and other reference materials referred to herein to describe the background of the invention and to provide additional detail regarding its practice are hereby incorporated by reference. For convenience, the reference materials are numerically referenced and grouped in the appended bibliography.

    摘要翻译： 一种用于遗传工程细胞产生可溶性和可分泌的高尔基体加工酶而不是天然存在的膜结合酶的方法。 细胞被遗传工程化以表达缺少膜锚定和保留信号的糖基转移酶。 所得到的改变的酶变得可溶并且可被细胞分泌而不失去其催化活性。 由细胞分泌可溶性糖基转移酶提供增加的糖基转移酶的产生和简化的恢复。

    Control of immune responses by modulating activity of glycosyltransferases
    17.
    发明授权
    Control of immune responses by modulating activity of glycosyltransferases 失效
    通过调节糖基转移酶的活性来控制免疫应答

    公开(公告)号：US06376475B1

    公开(公告)日：2002-04-23

    申请号：US09087117

    申请日：1998-05-29

    申请人： Jamey D. Marth ,  James C. Paulson

    发明人： Jamey D. Marth ,  James C. Paulson

    IPC分类号： C12Q148

    CPC分类号： C12N15/1137 ,  A01K67/0271 ,  A01K2217/075 ,  C12N9/1081

    摘要： The invention provides methods for inhibiting immune responses by inhibiting the biosynthesis of the sialyl galactosides that are involved in immune responses. In particular, B lymphocyte-mediated immune responses are mediated by interfering with synthesis of &agr;2,6 sialylgalactosides, while T lymphocyte-mediated immune responses are inhibited by blocking synthesis of &agr;2,3 sialylgalactosides. The inhibition is accomplished by, for example, inhibiting the activity of a glycosyltransferase involved in synthesis of the respective sialyl galactoside.

    摘要翻译： 本发明提供了通过抑制参与免疫应答的唾液酸半乳糖苷的生物合成来抑制免疫应答的方法。 特别地，通过干扰α2,6唾液酸半乳糖苷的合成来介导B淋巴细胞介导的免疫应答，而通过阻断α2,3唾液酸半乳糖苷的合成来抑制T淋巴细胞介导的免疫应答。 通过例如抑制参与合成唾液酸半乳糖苷的糖基转移酶的活性来实现抑制。