摘要:
Methods of obtaining N-terminal methionine-free proteins are described. The methods employ a novel enzyme, E. coli methionine aminopeptidase either in vitro or in vivo. For in vivo application, plasmid-borne DNA encoding the peptidase is transformed into a bacterial host which produces the desired protein.
摘要:
A method and a cloning vector are described for the controlled accumulation of cloned heterologous gene products in Bacillus subtilis. The cloning vector is capable of being replicated in B. subtilis and includes the heterologous gene located and oriented such as to be under the control of an operator, promoter, and ribosomal binding site sequence. The gene codes for a protein which is under the control of a transport mechanism by which the protein is secreted by the B. subtilis. The gene product is recovered from the growth medium for the B. subtilis. The cloning vector is also capable of similar use in other bacteria such as E. coli.
摘要:
Chimeric plasmids capable of transforming bacteria and yeast are described. The plasmids carry the Cm (chloramphenicol resistance) gene and the Tc (tetracycline resistance) gene as selectable markers. The Cm gene allows the plasmids to be selected for in wild-type strains of the yeast Saccharomyces cerevisiae. The Tc gene allows heterologous genes cloned into the plasmids to be selected for in Escherichia coli bacteria.
摘要:
A white line skipping technique for data reduction is disclosed for reducing facsimile transmission time. Video processing for white line skipping centers around the use of a five element solid state linear array. The photosensitive area for each of the five elements corresponds to a single picture element for the defined resolution parameters. VID 0 is the active video signal and is processed in the normal manner for transmission. VID 1 through VID 4 comprise the look ahead scan elements. The slicing level is derived from the VID 0 peak detector which controls the video automatic gain control. Any black video elements encountered during a scan line causes a flip-flop to be set. At the end of each scan line, corresponding to one complete drum revolution, and during the lost time interval, the status of each flip-flop is sampled by the microprocessor to determine whether the scan line is entire white. The flip-flops are then reset for the next scan or drum revolution.
摘要翻译:公开了用于减少传真传输时间的白线跳过技术。 用于白线跳过的视频处理围绕使用五元素固态线性阵列。 五个元件中的每一个的光敏区域对应于用于定义的分辨率参数的单个图像元素。 VID 0是有效的视频信号,并以正常方式进行处理以进行传输。 VID 1至VID 4包括前瞻扫描元件。 切片电平从控制视频自动增益控制的VID 0峰值检测器导出。 在扫描线期间遇到的任何黑色视频元件都会触发一个触发器。 在每个扫描线的末端,对应于一个完整的转鼓旋转,并且在丢失的时间间隔期间,每个触发器的状态由微处理器采样,以确定扫描线是否是整个白色。 然后复位触发器进行下一次扫描或转鼓旋转。
摘要:
A light emitting diode reflector molding process, and a construction thereof includes preparation of a first and a second green sheet structures, the first green sheet structure being provided with a first pattern; the second green sheet structure being placed on top of the first green sheet structure; a metallic layer being coated on the second green sheet structure, the second green sheet structure being molded along the opening pattern of and covering upon the first green sheet for the metallic layer to become the wall of the reflector opening.
摘要:
A light emitting diode reflector molding process, and a construction thereof includes preparation of a first and a second green sheet structures respectively provided with a first and a second open patterns with the porosity of the second open pattern smaller than that of the first open pattern; the second green sheet structure being placed on top of the first green sheet structure to such that both opening patterns being overlapped to each; a metallic layer being coated on the second green sheet structure, the second green sheet structure being molded along the opening pattern of and covering upon the first green sheet for the metallic layer to become the wall of the reflector opening.
摘要:
A memory device and the method for manufacturing same is disclosed. The device comprises a first oxide layer on top of a substrate, a floating gate layer on top of the first oxide layer, a second oxide layer over the floating gate layer, wherein the second oxide layer and the floating gate layer have a first opening and a second opening respectively, and wherein the width of second opening is bigger than the width of the narrowest region of the first opening so that the floating gate layer is pulled back horizontally underneath the second oxide layer. A source region is in the substrate underneath the first oxide layer, and a third oxide layer fills in the first and second openings conforming to the contour thereof, wherein the third oxide has a third opening to reach a portion of the source region. Further, a control gate material fills in the third opening.
摘要:
A non-volatile memory is described. A substrate comprising a stacked layer is provided. A sacrificial layer is deposited and patterned to form a first opening. A first spacer is formed on sidewalls of the first opening, and the stacked layer is etched using the first spacer as a first mask to form a second opening. An isolation layer is formed in a portion of the first and the second openings, and a conductive filling layer is formed thereon. The stacked layer is etched using a portion of the conductive filling layer as a second mask.
摘要:
A memory device and the method for manufacturing the same is disclosed. The device includes a first oxide layer on top of a substrate, a floating gate layer on top of the first oxide layer, and a second oxide layer over the floating gate layer. The second oxide layer and the floating gate layer have a first opening and a second opening respectively wherein the width of second opening is bigger than the width of the narrowest region of the first opening so that the floating gate layer is pulled back horizontally underneath the second oxide layer. A source region is in the substrate underneath the first oxide layer, and a third oxide layer fills in the first and second openings conforming to the contour thereof. The third oxide has a third opening to reach a portion of the source region. Further, a control gate material fills in the third opening.