公开(公告)号：US10202597B2

公开(公告)日：2019-02-12

申请号：US15236021

申请日：2016-08-12

Applicant: Accelerate Diagnostics, Inc.

Inventor： Steven W. Metzger ,  Kenneth Robert Hance

IPC: C12N13/00 ,  B03C5/00 ,  C12M1/00 ,  C12Q1/02 ,  G01N1/34 ,  G02B21/36 ,  C12Q1/24 ,  G01N33/49 ,  C12N7/02 ,  C12N7/00

Abstract: Methods and systems for purifying cells and/or viruses are provided. The sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the cells and/or viruses in the well. The cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the cells and/or viruses may be retained in the well by embedding in the medium. The medium including the embedded cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface.

公开(公告)号：US20180080932A1

公开(公告)日：2018-03-22

申请号：US15827187

申请日：2017-11-30

Applicant: Accelerate Diagnostics, Inc.

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: G01N33/569 ,  G01N33/561 ,  G01N33/543 ,  B82Y5/00 ,  B82Y20/00 ,  B82Y10/00 ,  C12Q1/18

CPC classification number: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/18 ,  G01N33/5438 ,  G01N33/561

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

公开(公告)号：US20140363823A1

公开(公告)日：2014-12-11

申请号：US13763446

申请日：2013-02-08

Applicant: ACCELERATE DIAGNOSTICS, INC.

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: G01N33/569

CPC classification number: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/18 ,  G01N33/5438 ,  G01N33/561

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

公开(公告)号：US11603550B2

公开(公告)日：2023-03-14

申请号：US15484250

申请日：2017-04-11

Applicant: Accelerate Diagnostics, Inc.

Inventor： Alena Shamsheyeva ,  David C. Howson ,  Steven W. Metzger

IPC: C12Q1/18 ,  C12Q1/02 ,  B01L3/00 ,  C12M1/34 ,  G16B5/00

Abstract: Systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance are disclosed.

公开(公告)号：US20180291419A1

公开(公告)日：2018-10-11

申请号：US16007071

申请日：2018-06-13

Applicant: Accelerate Diagnostics, Inc.

Inventor： William L. Richards ,  Austin Ashby ,  Matthew Ketterer ,  Kevin Marshall ,  Josh Harrison ,  Matthew Mette ,  Paul Richards ,  Wayne Showalter ,  Jasmin Cote ,  Steven W. Metzger ,  Ken Hance ,  Meghan Mensack ,  Carlos Michel ,  Elke Allers ,  Dulini Gamage ,  Landon Prisbrey ,  Oleg Gusyatin ,  Alena Shamsheyeva ,  Ben Turng ,  Andrew Ghusson ,  Kurt Reinhardt ,  Phillip C. Halbert ,  Solene Bourgeois

IPC: C12Q1/02 ,  G02B7/28 ,  C12Q1/04 ,  G01N1/38 ,  G01N15/06 ,  B01L3/00 ,  G01N35/02 ,  G01N35/10 ,  H04N5/232 ,  G01N21/64 ,  G01N35/00 ,  G01N35/04

Abstract: A system for automated microorganism identification and antibiotic susceptibility testing comprising a reagent cartridge, a reagent stage, a cassette, a cassette, stage, a pipettor assembly, an optical detection system, and a controller is disclosed. The system is designed to dynamically adjust motor idle torque to control heat load and employs a fast focus process for determining the true focus position of an individual microorganism. The system also may quantify the relative abundance of viable microorganisms in a sample using dynamic dilution, and facilitate growth of microorganisms in customized media for rapid, accurate antimicrobial susceptibility testing.

公开(公告)号：US10023895B2

公开(公告)日：2018-07-17

申请号：US15085953

申请日：2016-03-30

Applicant: Accelerate Diagnostics, Inc.

Inventor： William L. Richards ,  Austin Ashby ,  Matthew Ketterer ,  Kevin Marshall ,  Josh Harrison ,  Matthew Mette ,  Paul Richards ,  Wayne Showalter ,  Jasmin Cote ,  Phillip C. Halbert ,  Solene Bourgeois ,  Steven W. Metzger ,  Ken Hance ,  Meghan Mensack ,  Carlos Michel ,  Elke Allers ,  Dulini Gamage ,  Landon Prisbrey ,  Oleg Gusyatin ,  Alena Shamsheyeva ,  Ben Turng ,  Andrew Ghusson ,  Kurt Reinhardt

IPC: C12Q1/18 ,  C12Q1/02 ,  B01L3/00 ,  C12Q1/04 ,  G01N1/38 ,  G01N35/02 ,  G01N35/10 ,  H04N5/232 ,  G02B7/28 ,  G01N15/06 ,  G01N21/64 ,  G01N35/04 ,  G01N35/00

Abstract: A system for automated microorganism identification and antibiotic susceptibility testing comprising a reagent cartridge, a reagent stage, a cassette, a cassette, stage, a pipettor assembly, an optical detection system, and a controller is disclosed. The system is designed to dynamically adjust motor idle torque to control heat load and employs a fast focus process for determining the true focus position of an individual microorganism. The system also may quantify the relative abundance of viable microorganisms in a sample using dynamic dilution, and facilitate growth of microorganisms in customized media for rapid, accurate antimicrobial susceptibility testing.

公开(公告)号：US09841422B2

公开(公告)日：2017-12-12

申请号：US14329387

申请日：2014-07-11

Applicant: Accelerate Diagnostics, Inc.

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: G01N33/53 ,  G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/18 ,  G01N33/543 ,  G01N33/561

CPC classification number: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/18 ,  G01N33/5438 ,  G01N33/561

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

公开(公告)号：US20170234781A1

公开(公告)日：2017-08-17

申请号：US15586132

申请日：2017-05-03

Applicant: Accelerate Diagnostics, Inc.

Inventor： Landon Prisbrey ,  Elise Blackmore ,  Kenneth R. Hance ,  Steven W. Metzger ,  Kevin Marshall

IPC: G01N1/40 ,  C12N13/00 ,  B03C5/00

CPC classification number: G01N1/40 ,  B03C5/005 ,  B03C2201/26 ,  C12M47/12 ,  C12N7/00 ,  C12N13/00 ,  C12N2710/00051 ,  C12Q1/02 ,  C12Q1/24 ,  G01N1/34 ,  G01N33/491 ,  G01N2001/4038 ,  G02B21/367

Abstract: Methods and systems for purifying one or more microbial cells and/or viruses from a biological sample are provided. The biological sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the microbial cells and/or viruses in the well. The microbial cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the microbial cells and/or viruses are retained in the well by embedding in the medium. The medium including the embedded microbial cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface. In some examples, a biological sample containing contaminants and one or more microbial cells is introduced to a well disposed in a porous filter medium, wherein the porous filter medium includes pores smaller than the one or more microbial cells, thereby preventing the one or more microbial cells from entering the porous filter medium.

公开(公告)号：US09677109B2

公开(公告)日：2017-06-13

申请号：US14209917

申请日：2014-03-13

Applicant: Accelerate Diagnostics, Inc.

Inventor： Alena Shamsheyeva ,  David C. Howson ,  Steven W. Metzger

IPC: C12Q1/18 ,  C12M1/34 ,  G06F19/12 ,  B01L3/00

CPC classification number: C12Q1/18 ,  B01L3/502715 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2300/024 ,  B01L2300/0864 ,  B01L2300/0867 ,  C12M41/36 ,  C12Q1/02 ,  G06F19/12

Abstract: This disclosure is related to systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance.

公开(公告)号：US20220099671A1

公开(公告)日：2022-03-31

申请号：US17318641

申请日：2021-05-12

Applicant: Accelerate Diagnostics, Inc.

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  G01N33/543 ,  G01N33/561 ,  C12Q1/18

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.