公开(公告)号：US09975919B2

公开(公告)日：2018-05-22

申请号：US14766129

申请日：2014-02-05

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon

IPC: C07K16/30 ,  B01D15/36 ,  B01D15/34 ,  C07K16/00 ,  C07K1/20 ,  C07K1/14 ,  C07K1/16 ,  B01D15/32 ,  B01D15/38

CPC classification number: C07K1/14 ,  B01D15/327 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3828 ,  C07K1/165 ,  C07K1/20 ,  C07K16/00 ,  C07K16/3015

Abstract: A method of reducing aggregate and product-contaminant complex content in a protein preparation including a desired protein includes (i) contacting the protein preparation with at least one solid surface comprising at least one surface-bound ligand capable of binding a metal, the surface-bound ligand being capable of binding a metal is initially substantially devoid of a metal, the operating conditions are selected to substantially prevent the binding of the desired protein to the at least one solid surface and (ii) separating the protein preparation from the surface-bound ligand, such that when more than one surface-bound ligand is present, each surface-bound ligand is independently either of the same net charge or charge neutral.

公开(公告)号：US20170218011A1

公开(公告)日：2017-08-03

申请号：US15455956

申请日：2017-03-10

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon

IPC: C07K1/16 ,  C12N7/00 ,  B01D15/36 ,  C07K14/75 ,  B01D15/34 ,  C07K16/00 ,  C07K14/755

CPC classification number: C07K14/75 ,  B01D15/08 ,  B01D15/166 ,  B01D15/1807 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3885 ,  C07K1/16 ,  C07K14/755 ,  C07K16/00 ,  C07K16/065 ,  C12N7/00 ,  C12N2795/00 ,  C12N2795/14151

Abstract: Materials and methods for use of constrained cohydration agents in the purification of biological materials such as antibodies, viruses, cells, and cellular organelles in connection with convective chromatography, fluidized bed or co-precipitation applications.

公开(公告)号：US09695216B2

公开(公告)日：2017-07-04

申请号：US14769245

申请日：2014-02-20

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon ,  Vincent Vagenende

IPC: C07K1/36 ,  B01D15/34 ,  B01D15/36 ,  C07K1/18 ,  C07K1/30 ,  C07K1/34 ,  B01D15/38

CPC classification number: C07K1/36 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/18 ,  C07K1/30 ,  C07K1/34

Abstract: A method includes (i) adding allantoin in a supersaturating amount to a protein preparation including a desired protein and at least one endotoxin as a contaminant, (ii) removing solids after the adding step to provide a sample for further purification by void exclusion chromatography on a packed particle bed of electropositive particles in a column, the packed particle bed having an interparticle volume, (iii) applying a sample volume to the packed particle bed, wherein the electropositive particles support void exclusion chromatography, and wherein the sample volume is not greater than the interparticle volume, and (iv) eluting a purified sample including the desired protein and a reduced amount of the endotoxin. The method is optionally carried out with only the allantoin treatment or only the void exclusion chromatography.

公开(公告)号：US10280195B2

公开(公告)日：2019-05-07

申请号：US15313363

申请日：2014-05-28

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon

IPC: C07K1/18 ,  B01D15/16 ,  B01D15/36 ,  B01J20/28 ,  B01J20/32 ,  B01J41/04 ,  B01J41/20 ,  B01D15/42

Abstract: Methods are provided for enhancing reduction of virus and viral DNA levels in protein preparations.

公开(公告)号：US10259851B2

公开(公告)日：2019-04-16

申请号：US15313329

申请日：2014-05-29

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon ,  Hui Theng Gan

IPC: C07K1/36 ,  B01D15/12 ,  B01D15/34 ,  B01D15/36 ,  C07K14/47

Abstract: Methods are provided for extracting DNA-compaction proteins from biological samples.

公开(公告)号：US10174076B2

公开(公告)日：2019-01-08

申请号：US14894993

申请日：2014-02-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon

IPC: C07K1/30 ,  C07K1/34 ,  C07K16/00 ,  C07K1/36 ,  C07K16/32

Abstract: A method of purifying a target protein includes contacting a cell culture harvest or a protein preparation including at least one target protein with at least one fatty acid having 8 to 10 carbon atoms to form a mixture, contacting the mixture with one or more solids to form a mixture, the one or more solids comprise a cationic functional group, a metal binding functional group, or both, the metal binding functional group including a nitrogen-containing moiety selected from (1) a polyamine, (2) an imine, (3) an N-heterocycle, (4) an amino acid, (5) an N-hydroxyamide, (6), an arylamine, and combinations thereof, and separating solid materials after contacting the mixture with the one or more solids to provide a solution comprising the target protein.

公开(公告)号：US10160784B2

公开(公告)日：2018-12-25

申请号：US15121676

申请日：2014-02-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon

IPC: C07K1/30 ,  C07K16/00 ,  C07K1/32

Abstract: A method of purifying a target antibody includes contacting a cell culture harvest or a protein preparation including at least one target antibody with at least one fatty acid having 7 to 10 carbon atoms to form a mixture, contacting this mixture with allantoin, and then separating solid materials to provide a solution comprising the target antibody. Solid materials can be removed by filtration, sedimentation or centrifugation, and the fatty acids can be enanthic, caprylic, pelargonic, nonenoic or capric acid. The invention is also directed to kits used to facilitate this method of antibody purification.

公开(公告)号：US09994611B2

公开(公告)日：2018-06-12

申请号：US14769098

申请日：2014-02-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon ,  Tze Yang Lee

IPC: C07K1/30 ,  C07K1/36 ,  C07K1/16 ,  C07K16/32 ,  C07K1/32

CPC classification number: C07K1/36 ,  C07K1/30 ,  C07K1/32 ,  C07K16/32

Abstract: Methods for the improved purification of antibodies and other proteins from protein preparations including the steps of conditioning the protein preparation by contacting it with multivalent organic ions, then applying the conditioned preparation to an adsorptive chromatography medium.

公开(公告)号：US09927427B2

公开(公告)日：2018-03-27

申请号：US15031660

申请日：2014-10-15

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon

IPC: G01N33/50 ,  A61K35/12 ,  B01D15/36

CPC classification number: G01N33/5076 ,  A61K35/12 ,  B01D15/364 ,  G01N33/5005

Abstract: A method of isolating exosomes includes conducting at least one purification step in the presence of an organic zwitterion having a molecular weight of less than about 350 Daltons, a buffering pK of a negatively charged portion of the organic zwitterion is at least one full pH unit below an operating pH at which the at least one purification step is conducted, and a buffering pK of the positively charged portion of the organic zwitterion is at least one full pH unit above the operating pH.