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36 条记录 (耗时 0.035 秒)

    Process for Preparing L-amino Acids
    14.
    发明申请
    Process for Preparing L-amino Acids 审中-公开
    L-氨基酸的制备方法

    公开(公告)号：US20070122832A1

    公开(公告)日：2007-05-31

    申请号：US11615860

    申请日：2006-12-22

    申请人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P13/08 ,  C12N9/10 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1205 ,  C07K14/34 ,  C12N9/10 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/225 ,  C12P13/227 ,  C12P13/24

    摘要： The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70％ b）编码多肽的多核苷酸，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）的多核苷酸互补的多核苷酸， 或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少有citA基因 以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Methods of making L-amino acids in coryneform bacteria using the sigE gene
    15.
    发明申请
    Methods of making L-amino acids in coryneform bacteria using the sigE gene 审中-公开
    使用sigE基因在棒状细菌中制备L-氨基酸的方法

    公开(公告)号：US20050221450A1

    公开(公告)日：2005-10-06

    申请号：US11137504

    申请日：2005-05-26

    申请人： Bettina Mockel ,  Thoams Hermann ,  Mike Farwick ,  Michael Binder ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Thoams Hermann ,  Mike Farwick ,  Michael Binder ,  Walter Pfefferle

    IPC分类号： C07K14/34 ,  C12P13/08 ,  C12P13/04 ,  C07H21/04 ,  C12N9/10 ,  C12N9/12 ,  C12N15/74

    CPC分类号： C12R1/15 ,  C07K14/34 ,  C12P13/08

    摘要： The present invention relates to an isolated polynucleotide from Corynebacterium glutamicum comprising a polynucleotide sequence chosen from the group consisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b); and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.

    摘要翻译： 本发明涉及来自谷氨酸棒杆菌的分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自（a）与编码包含氨基酸的多肽的多核苷酸的至少70％的程度相同的多核苷酸 SEQ ID NO：2的序列; （b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％的程度相同的氨基酸序列; （c）与（a）或（b）的多核苷酸互补的多核苷酸; 和（d）包含（a），（b）或（c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少 sigE基因以增强形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Nucleotide sequences encoding the ptsH gene
    16.
    发明授权
    Nucleotide sequences encoding the ptsH gene 有权
    编码ptsH基因的核苷酸序列

    公开(公告)号：US06818432B2

    公开(公告)日：2004-11-16

    申请号：US09819930

    申请日：2001-03-29

    申请人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    发明人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    IPC分类号： C12N120

    CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.

    摘要翻译： 本发明涉及含有多核苷酸序列的分离的多核苷酸，所述多核苷酸序列选自包含a）多核苷酸的多核苷酸，所述多核苷酸与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸具有至少70％的同一性。 2，b）编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）多核苷酸，其包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸，以及用于增强编码磷酸转移酶系统的组分H的ptsH基因的L-氨基酸的发酵生产的方法， 以及使用上述多核苷酸作为引物或杂交探针。

    Nucleotide sequences which encode the pfk gene
    17.
    发明申请
    Nucleotide sequences which encode the pfk gene 审中-公开
    编码pfk基因的核苷酸序列

    公开(公告)号：US20070054379A1

    公开(公告)日：2007-03-08

    申请号：US10926293

    申请日：2004-08-26

    申请人： Bettina Mockel ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Walter Pfefferle

    IPC分类号： C12P13/08 ,  C12N9/10 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

    摘要： The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been Corynebacterium glutamicum, and a process for the production of an L-amino acid comprising culturing a coryneform bacteria comprising an overexpressed pfk gene, wherein said pfk gene comprises a polynucleotide having a nucleotide sequence which is at least 90% identical to the nucleotide of SEQ ID NO: 1 encoding a polypeptide having the enzymatic activity of a phosphofructokinase, accumulating said L-amino acid in the medium or in the cells of said bacterium, and isolated said L-amino acid.

    摘要翻译： 本发明涉及编码已经是谷氨酸棒杆菌的磷酸果糖激酶的核苷酸序列，以及L-氨基酸的生产方法，包括培养包含过表达的pfk基因的棒状细菌，其中所述pfk基因包含具有核苷酸的多核苷酸 序列，其与编码具有磷酸果糖激酶的酶活性的多肽的SEQ ID NO：1的核苷酸至少90％相同，在培养基中或所述细菌的细胞中积累所述L-氨基酸， 氨基酸。

    Nucleotide sequences encoding the ptsH gene
    19.
    发明申请
    Nucleotide sequences encoding the ptsH gene 有权
    编码ptsH基因的核苷酸序列

    公开(公告)号：US20050009143A1

    公开(公告)日：2005-01-13

    申请号：US10916437

    申请日：2004-08-12

    申请人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    发明人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    IPC分类号： C12N1/21 ,  C12N9/12 ,  C12P13/04 ,  C12P13/08 ,  C07H21/04 ,  C12N1/20 ,  C12N9/10

    CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.

    摘要翻译： 本发明涉及含有多核苷酸序列的分离的多核苷酸序列，所述多核苷酸序列选自以下组：a）多核苷酸，其与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸具有至少70％的相同性。 2，b）编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及用于增加编码磷酸转移酶系统的组分H的ptsH基因的L-氨基酸的发酵生产的方法 ，以及使用上述多核苷酸作为引物或杂交探针。