公开(公告)号：US09874575B2

公开(公告)日：2018-01-23

申请号：US14367230

申请日：2013-01-16

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Subhasish Purkayastha

IPC: G01N33/74 ,  G01N33/58 ,  G01N30/72

CPC classification number: G01N33/743 ,  G01N30/7233 ,  G01N33/58 ,  G01N2560/00 ,  Y10T436/18 ,  Y10T436/200833 ,  Y10T436/203332

Abstract: A method is described for mass spectrometric analysis of a sample comprising phenolic OH, such as a steroid comprising a phenolic OH, using a quaternary amino oxy Cookson (QAOC) reagent. The QAOC reagent can improve ionization and fragmentation properties of phenolic OH samples, which can thereby improve quantitation and identification. The method can include derivatizing the phenolic OH sample with the QAOC reagent to create an adduct and analyzing the adduct using mass spectrometry. Derivatization of the sample can be a one-step reaction where the QAOC reagent comprises an aminooxy MS tag or can be a multi-step reaction, where the adduct is formed by the reaction of carbonyl substituted PTAD based reagent and the sample followed by combination with an aminooxy MS tag. The sample can also be enriched prior to reacting it with the reagent. The method can also allow for multiplexing.

公开(公告)号：US20170115258A1

公开(公告)日：2017-04-27

申请号：US15339119

申请日：2016-10-31

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillai ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N30/72 ,  G01N33/74

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US09500622B2

公开(公告)日：2016-11-22

申请号：US13897628

申请日：2013-05-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N24/00 ,  G01N27/62 ,  G01N30/72 ,  G01N33/64 ,  G01N33/74 ,  G01N30/04 ,  G01N30/88 ,  H01J49/00

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。

公开(公告)号：US09188596B2

公开(公告)日：2015-11-17

申请号：US14104117

申请日：2013-12-12

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha

IPC: G01N33/82 ,  G01N30/72

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.

Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。

公开(公告)号：US20250136810A1

公开(公告)日：2025-05-01

申请号：US18683343

申请日：2022-08-11

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: C08L71/02 ,  H01J49/00

Abstract: The disclosure provides compositions, methods, and kits that find use in calibrating a mass spectrometer, and can include one or more predetermined concentration(s) of one or more calibrant molecule(s) that comprise a polyethylene glycol (PEG) compounds that have a single functional group that can be ionized by an ion source, and a solvent for dissolving the calibrant molecule(s). The calibrant molecule(s) and compositions including them can be used in either positive or negative ionization mode, and can be used for calibrating a variety of mass spectrometers (e.g., APCI, ESI) operating in a variety of acquisition modes (e.g., MRM, MS/MS, etc.).

公开(公告)号：US20230236189A1

公开(公告)日：2023-07-27

申请号：US18151974

申请日：2023-01-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: G01N33/569 ,  C12Q1/02 ,  G01N30/72 ,  C12Q1/68 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/88 ,  G01N33/68

CPC classification number: G01N33/569 ,  C12Q1/02 ,  G01N30/72 ,  C12Q1/68 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/6848 ,  G01N2030/8831

Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to fragment one or more nucleic acid primers labeled with a first tag and monitor for an intensity of the first tag in a mass spectrometry (MS) method. An ion source provides a beam of ions from a polymerase chain reaction amplified sample that includes one or more nucleic acid primers labeled with the first tag. The first tag binds to one or more nucleic acid primers of a known microbe and is cleaved from the nucleic acid primers during the MS method. The mass spectrometer receives the beam of ions and is adapted to perform the MS method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample.

公开(公告)号：US10094809B2

公开(公告)日：2018-10-09

申请号：US15339119

申请日：2016-10-31

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillai ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N24/00 ,  G01N30/72 ,  G01N33/74 ,  H01J49/16 ,  H01J49/00

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US20170052180A1

公开(公告)日：2017-02-23

申请号：US15118850

申请日：2015-02-25

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: G01N33/569 ,  G01N30/88 ,  C12Q1/04 ,  G01N30/72 ,  C12Q1/68 ,  G01N33/68

CPC classification number: G01N33/569 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/6848 ,  G01N2030/8831

Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to select a peptide labeled with a first tag of a known microbe, fragment the labeled peptide of the known microbe, and monitor for an intensity of the first tag in an MRM method using a processor. An ion source provides a beam of ions from a sample that includes peptides labeled with the first tag. The first tag binds to a peptide of a known microbe and is cleaved from the peptide of the known microbe during mass spectrometry. The mass spectrometer receives the beam of ions and is adapted to perform the MRM method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample using the processor.

Abstract translation: 提供了使用可切割标签进行微生物鉴定的系统和方法。 将控制信息发送到质谱仪以选择用已知微生物的第一标签标记的肽，对已知微生物的标记肽进行片段化，并使用处理器以MRM方法监测第一标签的强度。 离子源提供来自样品的离子束，其包括用第一标签标记的肽。 第一个标签结合已知微生物的肽，并且在质谱期间从已知微生物的肽切割。 质谱仪接收离子束，适用于在离子束上执行MRM方法。 如果从质谱仪接收的第一标签的强度超过阈值，则使用处理器在样品中识别已知的微生物。

公开(公告)号：US20150051111A1

公开(公告)日：2015-02-19

申请号：US14367230

申请日：2013-01-16

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Subhasish Purkayastha

IPC: G01N33/74 ,  G01N33/58

CPC classification number: G01N33/743 ,  G01N30/7233 ,  G01N33/58 ,  G01N2560/00 ,  Y10T436/18 ,  Y10T436/200833 ,  Y10T436/203332

Abstract: A method is described for mass spectrometric analysis of a sample comprising phenolic OH, such as a steroid comprising a phenolic OH, using a quaternary amino oxy Cookson (QAOC) reagent. The QAOC reagent can improve ionization and fragmentation properties of phenolic OH samples, which can thereby improve quantitation and identification. The method can include derivatizing the phenolic OH sample with the QAOC reagent to create an adduct and analyzing the adduct using mass spectrometry. Derivatization of the sample can be a one-step reaction where the QAOC reagent comprises an aminooxy MS tag or can be a multi-step reaction, where the adduct is formed by the reaction of carbonyl substituted PTAD based reagent and the sample followed by combination with an aminooxy MS tag. The sample can also be enriched prior to reacting it with the reagent. The method can also allow for multiplexing.

Abstract translation: 描述了使用季氨氧基Cookson（QAOC）试剂对包含酚类OH的样品（例如包含酚类OH）的类固醇进行质谱分析的方法。 QAOC试剂可以改善酚类OH样品的电离和断裂性能，从而提高定量和鉴定。 该方法可以包括用QAOC试剂衍生酚OH样品以产生加合物，并使用质谱分析加合物。 样品的衍生化可以是一步反应，其中QAOC试剂包含氨氧基MS标签或可以是多步反应，其中加合物通过羰基取代的基于PTAD的试剂与样品的反应形成，然后与 氨氧基MS标签。 在将样品与试剂反应之前也可以富集样品。 该方法还可以允许复用。