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公开(公告)号：US20050233373A1
公开(公告)日：2005-10-20
申请号：US11155656
申请日：2005-06-20
申请人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
CPC分类号： C12N9/1007 , A23K20/142 , C12P13/12 , C12R1/15
摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.
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12.发明授权Isolated polynucleotide from Corynebacterium encoding a homocysteine methyltransferase 有权来自编码高半胱氨酸甲基转移酶的棒状杆菌的分离的多核苷酸公开(公告)号：US06942996B2
公开(公告)日：2005-09-13
申请号：US09919835
申请日：2001-08-02
申请人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
CPC分类号： C12N9/1007 , A23K20/142 , C07K14/34 , C12P13/12
摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID. No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and p1 d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：1的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸。 b）编码包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或 b）和p1 d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因是 以增强形式存在，以及使用多核苷酸序列作为杂交探针。
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公开(公告)号：US20050064551A1
公开(公告)日：2005-03-24
申请号：US10838245
申请日：2004-05-05
申请人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.
摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。
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14.发明授权Nucleotide sequences which code for the cysD, cysN, cysK, cysE and cysH genes 有权编码cysD，cysN，cysK，cysE和cysH基因的核苷酸序列公开(公告)号：US06822085B2
公开(公告)日：2004-11-23
申请号：US09962357
申请日：2001-09-26
申请人： Mike Farwick , Klaus Huthmacher , Natalie Schischka , Brigitte Bathe , Walter Pfefferle , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Mike Farwick , Klaus Huthmacher , Natalie Schischka , Brigitte Bathe , Walter Pfefferle , Michael Binder , Dieter Greissinger , Georg Thierbach
IPC分类号： C07H2104
CPC分类号： C12N9/0051 , A23K10/12 , A23K20/142 , A23K40/10 , C12N9/1029 , C12N9/1241 , C12N9/88 , C12P13/08 , C12P13/12
摘要： Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.
摘要翻译： 来自编码cysD，cysN，cysK，cysE和cysH基因的棒状细菌的核苷酸序列和使用其中所述基因的细菌发酵制备氨基酸的方法，L-氨基酸的发酵制备方法 使用其中至少cysD基因，cysN基因，cysK基因，cysE基因和/或cysH基因至少以增强形式存在的棒状细菌，以及含有根据本发明的序列的多核苷酸作为杂交探针 以及从发酵液中制备含有L-甲硫氨酸的动物饲料添加剂的方法。
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公开(公告)号：US06812016B2
公开(公告)日：2004-11-02
申请号：US09919932
申请日：2001-08-02
申请人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
IPC分类号： C12N914
摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.
摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。
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公开(公告)号：US06479084B2
公开(公告)日：2002-11-12
申请号：US10058767
申请日：2002-01-30
IPC分类号： A23L1302
CPC分类号： A23K20/174 , A23K40/10 , Y10S426/807
摘要： A composition including at least one of D-pantothenic acid and a salt of D-panthothenic acid, greater than 50 percent of non-biomass constituents of a D-pantothenic fermentation liquor, and 0 to 100% of the biomass from D-pantothenic fermentation liquor wherein composition is a solid, pourable composition in the form of particles.
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17.发明授权Pourable feed additives containing D-pantothenic acid and/or salts thereof, and process for their preparation 有权含有D-泛酸和/或其盐的可浇注饲料添加剂及其制备方法公开(公告)号：US06368644B1
公开(公告)日：2002-04-09
申请号：US09895318
申请日：2001-07-02
IPC分类号： A23L1302
CPC分类号： A23K20/174 , A23K40/10 , Y10S426/807
摘要： A composition including at least one of D-pantothenic acid and a salt of D-panthothenic acid, greater than 50 percent of non-biomass constituents of a D-pantothenic fermentation liquor, and 0 to 100% of the biomass from D-pantothenic fermentation liquor wherein composition is a solid, pourable composition in the form of particles.
摘要翻译： 包含D-泛酸和D-泛酸盐中的至少一种，D-泛酸发酵液的非生物量成分的50％以及来自D-泛酸发酵的0-100％的生物质的组合物 其中组合物为颗粒形式的固体可倾倒组合物。
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18.发明授权Animal feed additive based on fermentation broth, a process for its production and its use 失效基于发酵液的动物饲料添加剂，其生产和使用的过程
公开(公告)号：US5622710A
公开(公告)日：1997-04-22
申请号：US518222
申请日：1995-08-23
申请人： Wolfram Binder , Franz-Ludwig Dahm , Ulrich Hertz , Heinz Friedrich , Hermann Lotter , Wolfgang Hohn , Dieter Greissinger , Wolfgang Polzer
发明人： Wolfram Binder , Franz-Ludwig Dahm , Ulrich Hertz , Heinz Friedrich , Hermann Lotter , Wolfgang Hohn , Dieter Greissinger , Wolfgang Polzer
CPC分类号： A23K20/142 , A23K10/12 , A23K40/10 , A23K40/20
摘要： An animal feed additive comprising stable pellets is produced by pelletizing from a fermentation broth containing an animal feed additive with at least 33% by weight of the fermentation products present. The pellets can be produced particularly effectively by pelletizing providing they are built up from at least 30% by weight of fine particles of which at least 70% by weight are smaller than 100 .mu.m in size. The biomass may be completely present, partly present or not present at all in the pellets.
摘要翻译： 包含稳定颗粒的动物饲料添加剂通过从含有至少33重量％存在的发酵产物的动物饲料添加剂的发酵液中造粒而产生。 颗粒可以通过造粒而特别有效地生产，只要它们由至少70重量％至少70重量％的小于100μm的细小颗粒构成。 生物质可以完全存在，部分存在或完全不存在于颗粒中。
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