公开(公告)号：US06822085B2

公开(公告)日：2004-11-23

申请号：US09962357

申请日：2001-09-26

申请人： Mike Farwick ,  Klaus Huthmacher ,  Natalie Schischka ,  Brigitte Bathe ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Mike Farwick ,  Klaus Huthmacher ,  Natalie Schischka ,  Brigitte Bathe ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C07H2104

CPC分类号： C12N9/0051 ,  A23K10/12 ,  A23K20/142 ,  A23K40/10 ,  C12N9/1029 ,  C12N9/1241 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12

摘要： Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.

摘要翻译： 来自编码cysD，cysN，cysK，cysE和cysH基因的棒状细菌的核苷酸序列和使用其中所述基因的细菌发酵制备氨基酸的方法，L-氨基酸的发酵制备方法 使用其中至少cysD基因，cysN基因，cysK基因，cysE基因和/或cysH基因至少以增强形式存在的棒状细菌，以及含有根据本发明的序列的多核苷酸作为杂交探针 以及从发酵液中制备含有L-甲硫氨酸的动物饲料添加剂的方法。

公开(公告)号：US20050233424A1

公开(公告)日：2005-10-20

申请号：US10898345

申请日：2004-07-26

申请人： Mike Farwick ,  Klaus Huthmacher ,  Natalie Schischka ,  Brigitte Bathe ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Mike Farwick ,  Klaus Huthmacher ,  Natalie Schischka ,  Brigitte Bathe ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： A23K1/00 ,  A23K1/16 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12 ,  C07H21/04 ,  C12N15/74

CPC分类号： C12N9/0051 ,  A23K10/12 ,  A23K20/142 ,  A23K40/10 ,  C12N9/1029 ,  C12N9/1241 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12

摘要： Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.

摘要翻译： 来自编码cysD，cysN，cysK，cysE和cysH基因的棒状细菌的核苷酸序列和使用其中所述基因的细菌发酵制备氨基酸的方法，L-氨基酸的发酵制备方法 使用其中至少cysD基因，cysN基因，cysK基因，cysE基因和/或cysH基因至少以增强形式存在的棒状细菌，以及含有根据本发明的序列的多核苷酸作为杂交探针 以及从发酵液中制备含有L-甲硫氨酸的动物饲料添加剂的方法。

公开(公告)号：US20050266535A1

公开(公告)日：2005-12-01

申请号：US11168476

申请日：2005-06-29

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach ,  Alfred Puehler

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach ,  Alfred Puehler

IPC分类号： A23K1/16 ,  C07K14/34 ,  C12N9/10 ,  C12P13/12 ,  C12P13/04 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C07K14/34 ,  C12P13/12

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因以增强形式存在，以及使用多核苷酸 序列作为杂交探针。

公开(公告)号：US06815196B2

公开(公告)日：2004-11-09

申请号：US09919831

申请日：2001-08-02

申请人： Brigitte Bathe ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N120

CPC分类号： C12N9/88 ,  C07K14/34 ,  C12P13/08 ,  C12P13/12

摘要： The present invention relates to isolated polynucleotides from Corynebacterium glutamicum encoding proteins of the transcription activator (MetR) and O-succinylhomoserine sulfhydrylase (MetZ). The invention also relates to producing L-amino acids, particularly methionine, in coryneform cells having attenuated metR and metZ genes.

摘要翻译： 本发明涉及编码转录激活子（MetR）和O-琥珀酰高丝氨酸巯基酸酶（MetZ）的蛋白质的谷氨酸棒杆菌分离的多核苷酸。 本发明还涉及在具有减毒的metR和metZ基因的棒状细胞中产生L-氨基酸，特别是甲硫氨酸。

公开(公告)号：US06958228B2

公开(公告)日：2005-10-25

申请号：US09919891

申请日：2001-08-02

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： A23K1/16 ,  C12N9/10 ,  C12P13/12 ,  C07H21/04 ,  C12N1/20 ,  C12N15/09

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C12P13/12 ,  C12R1/15

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metH基因以增强形式存在，以及使用多核苷酸序列 作为杂交探针。

公开(公告)号：US20050233373A1

公开(公告)日：2005-10-20

申请号：US11155656

申请日：2005-06-20

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： A23K1/16 ,  C12N9/10 ,  C12P13/12 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/74 ,  C12P13/04

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C12P13/12 ,  C12R1/15

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.

公开(公告)号：US06942996B2

公开(公告)日：2005-09-13

申请号：US09919835

申请日：2001-08-02

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： A23K1/16 ,  C07K14/34 ,  C12N9/10 ,  C12P13/12 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C07K14/34 ,  C12P13/12

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID. No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and p1 d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：1的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸。 b）编码包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或 b）和p1 d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因是 以增强形式存在，以及使用多核苷酸序列作为杂交探针。

公开(公告)号：US06759224B2

公开(公告)日：2004-07-06

申请号：US09919854

申请日：2001-08-02

申请人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N914

CPC分类号： C12N9/88 ,  A23K10/12 ,  A23K20/142 ,  A23K40/35 ,  A23K50/10 ,  C12P13/08 ,  C12P13/12

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 多肽，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含至少15个连续的多核苷酸的多核苷酸 a），b）或c）的多核苷酸序列的核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sahH基因以增强形式存在，以及使用多核苷酸 含有根据本发明的序列作为杂交探针。

公开(公告)号：US06812016B2

公开(公告)日：2004-11-02

申请号：US09919932

申请日：2001-08-02

申请人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N914

CPC分类号： C12P13/12 ,  C12N9/88 ,  C12P13/08

摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.

摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。

公开(公告)号：US20050064551A1

公开(公告)日：2005-03-24

申请号：US10838245

申请日：2004-05-05

申请人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N1/21 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12 ,  C12N9/12 ,  C07H21/04 ,  C12N9/16 ,  C12N15/74

CPC分类号： C12P13/12 ,  C12N9/88 ,  C12P13/08

摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.

摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。