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    Methods and compositions for extraction and storage of nucleic acids
    11.
    发明授权
    Methods and compositions for extraction and storage of nucleic acids 有权

    公开(公告)号：US08981077B2

    公开(公告)日：2015-03-17

    申请号：US13968497

    申请日：2013-08-16

    Applicant: General Electric Company

    Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

    IPC: C12N15/10 ,  B01J20/24

    Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

    METHODS AND COMPOSITIONS FOR EXTRACTION AND STORAGE OF NUCLEIC ACIDS
    13.
    发明申请
    METHODS AND COMPOSITIONS FOR EXTRACTION AND STORAGE OF NUCLEIC ACIDS 有权
    提取和储存核酸的方法和组合物

    公开(公告)号：US20130338351A1

    公开(公告)日：2013-12-19

    申请号：US13968497

    申请日：2013-08-16

    Applicant: General Electric Company

    Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

    IPC: B01J20/24 ,  C12N15/10

    CPC classification number: B01J20/24 ,  C12N15/1003 ,  C12N15/1006

    Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

    Abstract translation: 提供了用于提取，稳定和储存核酸的固体基质。 至少一种蛋白质变性剂和至少一种酸或酸滴定的缓冲剂在基质中以干燥状态浸渍; 并且所述基质配置成在水合时提供酸性pH。 该基质构造成从样品中提取核酸，并在环境条件下长时间地以干燥形式稳定所提取的核酸，特别是RNA。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。

    Substrates and associated methods for elution of nucleic acids
    16.
    发明授权
    Substrates and associated methods for elution of nucleic acids 有权
    底物和相关的核酸洗脱方法

    公开(公告)号：US09534214B2

    公开(公告)日：2017-01-03

    申请号：US14068633

    申请日：2013-10-31

    Applicant: General Electric Company

    Inventor： Bing Li ,  Gregory Andrew Grossmann ,  Erik Leeming Kvam ,  Brian Christopher Bales ,  Jason Louis Davis

    IPC: C12N15/10 ,  B01J20/22 ,  B01J20/26

    CPC classification number: C12N15/1006 ,  B01J20/22 ,  B01J20/261 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3255 ,  B01J20/3274 ,  C12N15/1017

    Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate is coated with saccharides, such as monosaccharides, oligosaccharides, polysaccharides or combinations thereof, and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.

    Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体底物涂覆有糖，例如单糖，寡糖，多糖或其组合，并且底物由一种或多种在基本干燥状态下浸渍的蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。

    SUBSTRATES AND ASSOCIATED METHODS FOR ELUTION OF NUCLEIC ACIDS
    19.
    发明申请
    SUBSTRATES AND ASSOCIATED METHODS FOR ELUTION OF NUCLEIC ACIDS 有权
    用于提取核酸的基质和相关方法

    公开(公告)号：US20150119566A1

    公开(公告)日：2015-04-30

    申请号：US14068633

    申请日：2013-10-31

    Applicant: General Electric Company

    Inventor： Bing Li ,  Gregory Andrew Grossmann ,  Erik Leeming Kvam ,  Brian Christopher Bales ,  Jason Louis Davis

    IPC: C12N15/10 ,  B01J20/26 ,  B01J20/22

    CPC classification number: C12N15/1006 ,  B01J20/22 ,  B01J20/261 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3255 ,  B01J20/3274 ,  C12N15/1017

    Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate is coated with saccharides, such as monosaccharides, oligosaccharides, polysaccharides or combinations thereof, and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.

    Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体底物涂覆有糖，例如单糖，寡糖，多糖或其组合，并且底物由一种或多种在基本干燥状态下浸渍的蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。

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