公开(公告)号：US10150976B2

公开(公告)日：2018-12-11

申请号：US14107832

申请日：2013-12-16

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P13/02 ,  C12P7/24 ,  C12P19/02 ,  C12P7/44 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  C12N15/52 ,  A23L29/00

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US10000758B2

公开(公告)日：2018-06-19

申请号：US15133150

申请日：2016-04-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12P7/42 ,  C08F120/06 ,  C08G63/664 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12P7/62

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

公开(公告)号：US20160237410A1

公开(公告)日：2016-08-18

申请号：US15027169

申请日：2014-10-03

Applicant: GENOMATICA, INC.

Inventor： Stefan Andrae ,  Michael Patrick Kuchinskas ,  Jingyi Li ,  Harish Nagarajan ,  Priti Pharkya

IPC: C12N9/04 ,  C12P7/24

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

Abstract translation: 本文描述了与其未修饰的对应物相比，能够分别甲醇或乙醇转化为甲醛或乙醛的至少两倍的非天然NAD +依赖性醇脱氢酶（ADH）。 描述了编码非天然醇脱氢酶的核酸，以及包含核酸的表达构建体和包含核酸或表达构建体的工程化细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，用于促进生产的方法 的靶产物或其中间体，包括靶产物或中间体的组合物，以及由目标产物或中间体制成的产品。

公开(公告)号：US11629363B2

公开(公告)日：2023-04-18

申请号：US15873798

申请日：2018-01-17

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P7/18 ,  C12P7/20 ,  C12P7/24 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52 ,  C12P7/04

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

公开(公告)号：US10563180B2

公开(公告)日：2020-02-18

申请号：US15027169

申请日：2014-10-03

Applicant: Genomatica Inc.

Inventor： Stefan Andrae ,  Michael Patrick Kuchinskas ,  Jingyi Li ,  Harish Nagarajan ,  Priti Pharkya

IPC: C12N9/04 ,  C12P7/24

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

公开(公告)号：US20190106701A1

公开(公告)日：2019-04-11

申请号：US15979313

申请日：2018-05-14

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12P7/62 ,  C12N15/63 ,  C08F120/06 ,  C12P7/42 ,  C12P7/40 ,  C12P7/24 ,  C08G63/664

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

公开(公告)号：US20180327788A1

公开(公告)日：2018-11-15

申请号：US15873798

申请日：2018-01-17

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/24 ,  C12N1/20 ,  C12N1/14 ,  C12N1/16 ,  C12N15/52 ,  C12P7/20 ,  C12N9/06 ,  C12N9/04 ,  C12P7/18 ,  C12P7/04 ,  C12N9/10

CPC classification number: C12P7/24 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

公开(公告)号：US09932611B2

公开(公告)日：2018-04-03

申请号：US14059285

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/46 ,  C07C55/10 ,  C08G63/00 ,  C12N15/70 ,  C12N15/81 ,  C12P7/24 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07C59/235 ,  C12N15/74 ,  C12N15/77

CPC classification number: C12P7/46 ,  C07C55/10 ,  C07C59/235 ,  C08G63/00 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/70 ,  C12N15/74 ,  C12N15/77 ,  C12N15/81 ,  C12N15/815 ,  C12P7/24 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.

公开(公告)号：US09346902B2

公开(公告)日：2016-05-24

申请号：US14071339

申请日：2013-11-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/42 ,  C08F120/06 ,  C08G63/664 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12N15/52

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物体如3-羟基异丁酸酯或MAA产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生3-羟基异丁酸酯或MAA的方法。

公开(公告)号：US20140302575A1

公开(公告)日：2014-10-09

申请号：US14071318

申请日：2013-11-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/24 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20

CPC classification number: C12P7/24 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于提高微生物生物体如1,2-丙二醇，正丙醇，1,3-丙二醇或甘油产生的有机化合物的产物产率。 本文还提供了使用这种生物体来生产1,2-丙二醇，正丙醇，1,3-丙二醇或甘油的方法。