公开(公告)号：US20170073691A1

公开(公告)日：2017-03-16

申请号：US15133150

申请日：2016-04-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Stephen J. VAN DIEN ,  Cara Ann TRACEWELL ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12N15/52 ,  C12N9/10 ,  C12N9/06 ,  C12P7/62 ,  C12N9/04

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物体如3-羟基异丁酸酯或MAA产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生3-羟基异丁酸酯或MAA的方法。

公开(公告)号：US20160355846A1

公开(公告)日：2016-12-08

申请号：US15148759

申请日：2016-05-06

Applicant: Genomatica, Inc.

Inventor： Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Robert HASELBECK ,  Catherine J. PUJOL-BAXLEY ,  Wei NIU ,  John D. TRAWICK ,  Harry YIM ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Jun SUN

IPC: C12P7/18 ,  C12N9/10 ,  C12N15/52 ,  C12N9/16 ,  C12N9/02 ,  C12N9/04 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Abstract translation: 本发明提供了包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化用于BDO的表达。 本发明还提供了使用这种微生物生产BDO的方法。

公开(公告)号：US20150368676A1

公开(公告)日：2015-12-24

申请号：US14603174

申请日：2015-01-22

Applicant: GENOMATICA, INC.

Inventor： Mark J. BURK ,  Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Wei NIU

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12P7/42 ,  C12N9/88

CPC classification number: C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

Abstract translation: 本发明提供了一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛 脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。 还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸 ，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸酯激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为 以足够的量表示产生1,4-丁二醇（BDO）。 另外提供了用于生产4-HB和BDO的方法。

公开(公告)号：US20140371417A1

公开(公告)日：2014-12-18

申请号：US14262461

申请日：2014-04-25

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCKINSKAS ,  Brian STEER

IPC: C12N9/04 ,  C12P17/04 ,  C12P13/00 ,  C12P19/32 ,  C12P7/18 ,  C12P7/62 ,  C12P7/24

CPC classification number: C12P7/18 ,  C07C31/207 ,  C07C47/19 ,  C07C211/09 ,  C07D307/33 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/13 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P17/04 ,  C12P19/32 ,  C12Y208/03

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

Abstract translation: 本发明提供具有4-羟基丁酸酯，γ-丁内酯，1,4-丁二醇，4-羟基丁醛，4-羟基丁酰辅酶A和/或腐胺途径的非天然存在的微生物，其能够产生4-羟基丁酸酯，其中 微生物生物体包含一种或多种遗传修饰。 本发明另外提供使用微生物生产4-羟基丁酸酯，γ-丁内酯，1,4-丁二醇，4-羟基丁醛，4-羟基丁酰-CoA和/或腐胺或相关产品的方法。