Sequencing near infrared and infrared fluorescence labeled DNA for
detecting using laser diodes
    14.
    发明授权
    Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes 失效
    测量近红外和红外荧光标记的DNA,用于使用激光二极管进行检测

    公开(公告)号:US5230781A

    公开(公告)日:1993-07-27

    申请号:US763230

    申请日:1991-09-20

    摘要: To sequence DNA automatically, DNA marked with near infrared fluorescent dyes are electrophoresed in a plurality of channels through a gel electrophoresis slab wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser diode and a sensor, wherein the laser scans with scanning light at a scanning light frequency within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission frequency of the marked DNA. The light is modulated from said laser at a predetermined modulation frequency and fluorescent light emitted by said DNA bands at said modulation frequency is detected, whereby background noise from the medium through which the light is transmitted is discriminated against.

    摘要翻译: 为了自动序列DNA,用近红外荧光染料标记的DNA通过凝胶电泳板在多个通道中进行电泳,其中根据凝胶电泳板中DNA片段的大小将DNA样品分解成荧光标记的DNA条带。 分离的样品用激光二极管和传感器进行光电扫描,其中激光以扫描光频率在所述荧光标记的DNA样品的吸收光谱内以扫描光扫描,并以标记的DNA的发射频率感测光。 以预定的调制频率从所述激光器调制光,并且检测由所述DNA条带以所述调制频率发射的荧光,从而区分来自发射光的介质的背景噪声。

    Gas producing electrolytic cell for portable devices
    15.
    发明授权
    Gas producing electrolytic cell for portable devices 失效
    用于便携式设备的气体生产电解槽

    公开(公告)号:US4332664A

    公开(公告)日:1982-06-01

    申请号:US185407

    申请日:1980-09-09

    IPC分类号: C25B9/00 C25B15/08

    CPC分类号: C25B9/00

    摘要: A gas producing electrolytic cell for portable devices comprising a vessel, an electrolyte, a cap, a gas space, channel labyrinths, a separating wall and contacts. The channel labyrinths eliminate the outflow of the electrolyte from the cell in its overturned position, tender the return of electrolyte to the vessel, to the original level possible. The contacts can be inundated by the electrolyte and in this way an outer electric circuit can be closed for cutting out the stream of electrolyse and for signalizating the overturned position of the cell.

    摘要翻译: 一种用于便携式设备的产气电解池,包括容器,电解质,盖,气体空间,通道迷宫,分隔壁和触点。 通道迷宫可以消除电解液在其翻转位置的流出,将电解液返回到容器,达到原来的水平。 触点可以被电解液淹没,并且以这种方式可以关闭外部电路以切出电解流并且用于发信号通知电池的翻转位置。

    Scanners for reading near infrared fluorescent marks
    16.
    发明授权
    Scanners for reading near infrared fluorescent marks 失效
    用于阅读近红外荧光标记的扫描仪

    公开(公告)号:US5959296A

    公开(公告)日:1999-09-28

    申请号:US880037

    申请日:1997-06-20

    IPC分类号: G02B6/38 G06K7/12 G06K7/10

    摘要: An apparatus for detecting an invisible, near infrared fluorescing mark disposed on a background comprises: includes an excitation source for exciting the mark, having sufficient power to excite the mark and provide a fluorescent signal the mark from a; a signal detector for detecting the fluorescent signal from the mark; a and power modulator means for varying the power if the excitation source between zero and the maximum power level to produce a decodable fluorescent signal from the mark disposed on the background. A process for decoding an invisible mark having a near infrared fluorescing material includes the steps of: providing at least one invisible, near infrared fluorescing mark disposed on at least one portion of a background; exciting the mark with a laser having a maximum power level sufficient to provide a fluorescent signal from the mark; detecting the fluorescent signal and concurrently varying the power of the laser between zero and its maximum power, to obtain a decodable fluorescent signal.

    摘要翻译: 用于检测设置在背景上的不可见的近红外荧光标记的装置包括:包括用于激发标记的激励源,具有足够的功率来激发标记并从a提供标记的荧光信号; 用于从标记检测荧光信号的信号检测器; a和功率调制器装置,用于如果激励源在零和最大功率水平之间改变功率,以从设置在背景上的标记产生可解码的荧光信号。 用于解码具有近红外荧光材料的不可见标记的方法包括以下步骤:提供设置在背景的至少一部分上的至少一个不可见的近红外荧光标记; 使用具有足以提供来自标记的荧光信号的最大功率电平的激光器来激发标记; 检测荧光信号并同时改变激光器在0和最大功率之间的功率,以获得可解码的荧光信号。

    Sequencing near infrared and infrared fluorescence labeled DNA for
detecting useing laser diodes
    19.
    发明授权
    Sequencing near infrared and infrared fluorescence labeled DNA for detecting useing laser diodes 失效
    测量使用激光二极管的近红外和红外荧光标记的DNA

    公开(公告)号:US5366603A

    公开(公告)日:1994-11-22

    申请号:US860140

    申请日:1992-03-30

    摘要: To sequence DNA automatically, DNA marked with near infrared fluorescent dyes are electrophoresed in a plurality of channels through a gel electrophoresis slab wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser diode and a sensor, wherein the laser scans with scanning light at a scanning light frequency within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission frequency of the marked DNA. The light is modulated from said laser at a predetermined modulation frequency and fluorescent light emitted by said DNA bands at said modulation frequency is detected, whereby background noise from the medium through which the light is transmitted is discriminated against.

    摘要翻译: 为了自动序列DNA,用近红外荧光染料标记的DNA通过凝胶电泳板在多个通道中进行电泳,其中根据凝胶电泳板中DNA片段的大小将DNA样品分解成荧光标记的DNA条带。 分离的样品用激光二极管和传感器进行光电扫描,其中激光以扫描光频率在所述荧光标记的DNA样品的吸收光谱内以扫描光扫描,并以标记的DNA的发射频率感测光。 以预定的调制频率从所述激光器调制光,并且检测由所述DNA条带以所述调制频率发射的荧光,从而区分来自发射光的介质的背景噪声。