公开(公告)号：US11740255B2

公开(公告)日：2023-08-29

申请号：US16616864

申请日：2018-11-09

Applicant: ILLUMINA, INC.

Inventor： Jennifer Olivia Foley ,  Darren Segale ,  Cyril Delattre

IPC: G01N35/10 ,  B01L3/02

CPC classification number: G01N35/1016 ,  B01L3/0265 ,  B01L2200/0689 ,  B01L2400/0427 ,  B01L2400/0622

Abstract: A system includes a droplet actuator having a droplet-operation gap between top and bottom substrates, a reservoir(s) external to and coupled to the droplet actuator, the reservoir(s) sized for a large-volume fluid, and pressure source(s) external to the droplet actuator and coupled to the at least one reservoir. Operation of the system includes filling the reservoir(s) with a large volume of fluid(s), dispensing droplet(s) of the fluid(s) to the droplet-operation gap using the pressure source(s) as part of performing a droplet operation(s). Movement of the droplet(s) may be effectuated by activating the droplet actuator.

公开(公告)号：US20230112203A1

公开(公告)日：2023-04-13

申请号：US17934175

申请日：2022-09-21

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Boyan Boyanov ,  Craig Ciesla ,  Anthony Flannery ,  Gerald Kreindl ,  Rean Silke Musa ,  Darren Segale

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N27/414 ,  B01L3/00

Abstract: Devices for sequencing biopolymers and methods of using the devices are disclosed. In one example, such a device has a nanopore, a plurality of wells and fluidic tunnels to allow a biopolymer to translocate in the device. In some embodiments, the device may include integrated electronics or micro-electromechanical systems, such as valves, bubble generators/annihilators or pressure pulse generators, to actively control fluidic/ionic/electric flows in the device.

公开(公告)号：US11565252B2

公开(公告)日：2023-01-31

申请号：US16706332

申请日：2019-12-06

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: B01L3/00 ,  C12Q1/6837 ,  B01J19/00 ,  B01L9/00

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

公开(公告)号：US20220280940A1

公开(公告)日：2022-09-08

申请号：US17750610

申请日：2022-05-23

Applicant: ILLUMINA, INC.

Inventor： Sz-Chin Steven Lin ,  Richard Lemoine ,  Rajagopal Panchapakesan ,  Wesley A. Cox-Muranami ,  Darren Segale

IPC: B01L3/00 ,  F16K99/00 ,  G01M3/28 ,  G01M3/26

Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.

公开(公告)号：US11351541B2

公开(公告)日：2022-06-07

申请号：US16500787

申请日：2018-04-02

Applicant: ILLUMINA, INC.

Inventor： Sz-Chin Steven Lin ,  Richard Lemoine ,  Rajagopal Panchapakesan ,  Wesley A. Cox-Muranami ,  Darren Segale

IPC: B01L3/00 ,  F16K99/00 ,  G01M3/28 ,  G01M3/26

Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.

公开(公告)号：US20250044230A1

公开(公告)日：2025-02-06

申请号：US18922150

申请日：2024-10-21

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  B01L3/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N27/414 ,  G01N33/543 ,  H01L27/00 ,  H01L27/088 ,  H01L27/146

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US12140543B2

公开(公告)日：2024-11-12

申请号：US18339185

申请日：2023-06-21

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  B01L3/00 ,  G01N21/64 ,  G01N27/414 ,  G01N33/543 ,  H01L27/00 ,  H01L27/088 ,  C12Q1/6874 ,  G01N21/05

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US11719637B2

公开(公告)日：2023-08-08

申请号：US17405379

申请日：2021-08-18

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  H01L27/00 ,  G01N27/414 ,  B01L3/00 ,  G01N33/543 ,  G01N21/64 ,  H01L27/088 ,  H01L29/78 ,  G01N21/05 ,  C12Q1/6874

CPC classification number: G01N21/645 ,  B01L3/502707 ,  B01L3/502715 ,  G01N21/6428 ,  G01N21/6454 ,  G01N27/4145 ,  G01N33/54373 ,  H01L27/00 ,  H01L27/088 ,  H01L27/14623 ,  C12Q1/6874 ,  G01N21/05 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/061 ,  G01N2201/0642

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US20210381977A1

公开(公告)日：2021-12-09

申请号：US17405379

申请日：2021-08-18

Applicant: ILLUMINA, INC.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  G01N33/543 ,  B01L3/00 ,  H01L27/146 ,  H01L27/088 ,  H01L27/00 ,  G01N27/414

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US11125691B2

公开(公告)日：2021-09-21

申请号：US16295973

申请日：2019-03-07

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  H01L27/00 ,  G01N27/414 ,  G01N33/543 ,  G01N21/64 ,  B01L3/00 ,  H01L27/088 ,  C12Q1/6874 ,  H01L29/78 ,  G01N21/05

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.