利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

37 条记录 (耗时 0.016 秒)

    OPTICAL DISTORTION CORRECTION FOR IMAGED SAMPLES
    12.
    发明申请
    OPTICAL DISTORTION CORRECTION FOR IMAGED SAMPLES 审中-公开

    公开(公告)号：US20180260940A1

    公开(公告)日：2018-09-13

    申请号：US15909437

    申请日：2018-03-01

    申请人： Illumina, Inc.

    发明人： Robert Langlois ,  Paul Belitz

    IPC分类号： G06T5/00 ,  G06T3/00 ,  G06K9/32 ,  G01B11/16 ,  H04N1/387

    CPC分类号： G06T5/006 ,  G01B11/16 ,  G01N21/6452 ,  G01N21/6486 ,  G06K9/32 ,  G06T3/0006 ,  G06T2207/30072 ,  H04N1/387 ,  H04N5/3572

    摘要： Techniques are described for dynamically correcting image distortion during imaging of a patterned sample having repeating spots. Different sets of image distortion correction coefficients may be calculated for different regions of a sample during a first imaging cycle of a multicycle imaging run and subsequently applied in real time to image data generated during subsequent cycles. In one implementation, image distortion correction coefficients may be calculated for an image of a patterned sample having repeated spots by: estimating an affine transform of the image; sharpening the image; and iteratively searching for an optimal set of distortion correction coefficients for the sharpened image, where iteratively searching for the optimal set of distortion correction coefficients for the sharpened image includes calculating a mean chastity for spot locations in the image, and where the estimated affine transform is applied during each iteration of the search.

    Apparatus and method of estimating values from images
    14.
    发明授权
    Apparatus and method of estimating values from images 有权

    公开(公告)号：US11768364B2

    公开(公告)日：2023-09-26

    申请号：US17110409

    申请日：2020-12-03

    申请人： ILLUMINA, INC.

    发明人： Robert Langlois ,  Bo Lu ,  Hongji Ren ,  Joseph Pinto ,  Simon Prince ,  Austin Corbett

    IPC分类号： G06T7/00 ,  G02B21/36 ,  G02B21/14

    CPC分类号： G02B21/367 ,  G02B21/14 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    摘要： A method is used to generate a distortion model for a structured illumination microscopy (SIM) optical system. A sliding window is moved in relation to a plurality of images to define a plurality of sub-tiles. Each sub-tile represents a portion of the corresponding image. Parameters are estimated for each sub-tiles. The parameters include two or more parameters selected from the group consisting of modulation, angle, spacing, phase offset, and phase deviation. A full width at half maximum (FWHM) value associated with each sub-tile is estimated. A distortion model is estimated, based at least in part on a combination of the estimated parameters and FWHM values stored in the predetermined format and an estimated center window parameter. A two-dimensional image may be generated, based at least in part on the estimated distortion model. The two-dimensional image may include representations indicating where distortions occur in the optical system.

    Optical distortion correction for imaged samples
    15.
    发明授权
    Optical distortion correction for imaged samples 有权

    公开(公告)号：US11568522B2

    公开(公告)日：2023-01-31

    申请号：US17162928

    申请日：2021-01-29

    申请人： Illumina, Inc.

    发明人： Robert Langlois ,  Paul Belitz

    IPC分类号： G06T5/00 ,  H04N5/357 ,  G01N21/64 ,  G02B21/00 ,  H04N1/401 ,  H04N1/409 ,  G06V10/24 ,  G06V20/69 ,  G01B11/16 ,  G06T3/00 ,  H04N1/387

    摘要： Techniques are described for dynamically correcting image distortion during imaging of a patterned sample having repeating spots. Different sets of image distortion correction coefficients may be calculated for different regions of a sample during a first imaging cycle of a multicycle imaging run and subsequently applied in real time to image data generated during subsequent cycles. In one implementation, image distortion correction coefficients may be calculated for an image of a patterned sample having repeated spots by: estimating an affine transform of the image; sharpening the image; and iteratively searching for an optimal set of distortion correction coefficients for the sharpened image, where iteratively searching for the optimal set of distortion correction coefficients for the sharpened image includes calculating a mean chastity for spot locations in the image, and where the estimated affine transform is applied during each iteration of the search.

    FIDUCIALS FOR USE IN REGISTRATION OF A PATTERNED SURFACE
    16.
    发明申请
    FIDUCIALS FOR USE IN REGISTRATION OF A PATTERNED SURFACE 有权

    公开(公告)号：US20220414853A1

    公开(公告)日：2022-12-29

    申请号：US17845519

    申请日：2022-06-21

    申请人： ILLUMINA, INC. ,  ILLUMINA SOFTWARE, INC.

    发明人： John S. Vieceli ,  Hui Han ,  Robert Langlois ,  Bo Lu

    IPC分类号： G06T7/00 ,  G01N15/14 ,  G06T3/00 ,  G06T7/30

    摘要： Registration of a patterned flow cell may utilize fiducials comprising sets or groupings of features (e.g., sites, sample wells, nanowells) having known locations and in which the placement of the features is not in accordance with a periodic pattern or is otherwise distinguishable from the periodic pattern of sites present in non-fiducial regions of the flow cell substrate. In certain embodiments the positioning of the sites that are part of the fiducial represent a break or discontinuity in the periodic pattern of sites that are otherwise present on the surface of a patterned flow cell.

    APPARATUS AND METHOD OF PROVIDING PARAMETER ESTIMATION
    17.
    发明申请
    APPARATUS AND METHOD OF PROVIDING PARAMETER ESTIMATION 有权

    公开(公告)号：US20220113532A1

    公开(公告)日：2022-04-14

    申请号：US17558829

    申请日：2021-12-22

    申请人： ILLUMINA, INC.

    发明人： Austin Corbett ,  Bo Lu ,  Robert Langlois ,  Joseph Pinto ,  Yu Chen ,  Peter Newman ,  Hongji Ren

    IPC分类号： G02B21/36 ,  G06T7/80 ,  G02B26/06 ,  G06T7/00 ,  H04N5/225

    摘要： A method is used to generate a report presenting parameter values corresponding to a structured illumination microscopy (SIM) optical system. The parameter values are based at least in part on the performed modulation calculation corresponding to an image set captured with the SIM optical system. A minimum FWHM slice is identified, based at least in part on an average FWHM value across the images in the first image set. Parameter estimation is performed on the identified minimum FWHM slice. Best in-focus parameters are identified based at least in part on the performed estimation. A phase estimate is performed for each image in the set. A modulation calculation is performed based at least in part on the identified best in-focus parameters. The report is based at least in part on the performed modulation calculation.

    PHASING CORRECTION
    18.
    发明申请
    PHASING CORRECTION 有权

    公开(公告)号：US20210389236A1

    公开(公告)日：2021-12-16

    申请号：US17446464

    申请日：2021-08-31

    申请人： Illumina, Inc.

    发明人： Robert Langlois ,  Paul Belitz

    IPC分类号： G01N21/25 ,  C12Q1/6874 ,  C12N15/10 ,  G01J3/02 ,  C12Q1/6855 ,  C12Q1/6806 ,  B01L3/00 ,  G16B30/00 ,  G16B25/00 ,  C12Q1/6869 ,  H04N13/15 ,  G01N15/14 ,  G16B30/10

    摘要： Memory efficient methods determine corrected color values from image data acquired by a nucleic acid sequencer during a base calling cycle. Such methods may: (a) obtain an image of a substrate (e.g., a portion of a flow cell) including a plurality of sites where nucleic acid bases are read; (b) measure color values of the plurality of sites from the image of the substrate; (c) store the color values in a processor buffer of the sequencer's one or more processors; (d) retrieve partially phase-corrected color values of the plurality of sites, where the partially phase-corrected color values were stored in the sequencer's memory during an immediately preceding base calling cycle; (e) determine a prephasing correction; and (f) determine the corrected color values. In various implementations, these operations are all performed during a single base calling cycle. In certain embodiments, the methods additionally include using the corrected color values to make base calls for the plurality of sites. Sequencers may be designed or configured to implement such methods.

    PHASING CORRECTION
    19.
    发明申请
    PHASING CORRECTION 审中-公开

    公开(公告)号：US20180195953A1

    公开(公告)日：2018-07-12

    申请号：US15863241

    申请日：2018-01-05

    申请人： Illumina, Inc.

    发明人： Robert Langlois ,  Paul Belitz

    IPC分类号： G01N21/25 ,  C12Q1/6874 ,  C12N15/10 ,  G01J3/02 ,  G06F19/22 ,  C12Q1/6855 ,  C12Q1/6806 ,  B01L3/00

    摘要： Memory efficient methods determine corrected color values from image data acquired by a nucleic acid sequencer during a base calling cycle. Such methods may: (a) obtain an image of a substrate (e.g., a portion of a flow cell) including a plurality of sites where nucleic acid bases are read; (b) measure color values of the plurality of sites from the image of the substrate; (c) store the color values in a processor buffer of the sequencer's one or more processors; (d) retrieve partially phase-corrected color values of the plurality of sites, where the partially phase-corrected color values were stored in the sequencer's memory during an immediately preceding base calling cycle; (e) determine a prephasing correction; and (f) determine the corrected color values. In various implementations, these operations are all performed during a single base calling cycle. In certain embodiments, the methods additionally include using the corrected color values to make base calls for the plurality of sites. Sequencers may be designed or configured to implement such methods.