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    Process and reagent for the specific determination of fructosamine content in blood and samples obtained from blood
    11.
    发明授权
    Process and reagent for the specific determination of fructosamine content in blood and samples obtained from blood 失效
    用于特异性测定血液中血凝素含量的过程和试剂和血液中获得的样品

    公开(公告)号：US5149633A

    公开(公告)日：1992-09-22

    申请号：US249381

    申请日：1988-09-26

    申请人： Bernd Vogt ,  Liselotte Schellong ,  Joachim Siedel ,  Joachim Ziegenhorn

    发明人： Bernd Vogt ,  Liselotte Schellong ,  Joachim Siedel ,  Joachim Ziegenhorn

    IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/30 ,  C12Q1/34 ,  G01N33/66 ,  G01N33/96

    CPC分类号： C12Q1/26 ,  C12Q1/34 ,  G01N33/66 ,  G01N33/96 ,  C12Q2326/92 ,  Y10S436/904 ,  Y10T436/144444 ,  Y10T436/173845 ,  Y10T436/25 ,  Y10T436/25125

    摘要： Process for the specific determination of the serum fructosamine content in blood or in samples obtained from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, in which before the color reaction sample components with a non-specific reducing action and/or causing turbidity are removed and subsequently the color reagent is added at a pH value of from 10 to 12. The sample components are removed by treatment at approximately neutral pH value with a reagent composition comprising at least one enzymatic oxidation agent, optionally together with peroxidase and/or catalase and/or lipase, as well as with at least one SH group-blocking substance. A kit for the specific determination of the serum fructosamine content in blood or samples obtained from blood, comprises said reagent composition, a rebuffering reagent with a buffer which has an alkaline pH value and a color reagent for the detection of fructosamine.

    摘要翻译： 通过与适当的着色剂反应，测定血液中或血液中得到的样品中的血清果糖胺含量的特异性测定方法，由此产生颜色变化，其中在着色反应之前，具有非特异性还原作用的样品成分 和/或引起浊度被除去，随后在10至12的pH值下加入显色试剂。通过用大约中性pH值处理的样品组分除去，试剂组合物包含至少一种酶氧化剂，任选地一起 与过氧化物酶和/或过氧化氢酶和/或脂肪酶，以及至少一种SH基团阻断物质。 用于特异性测定血液或血液中的血清果糖胺含量的试剂盒包括所述试剂组合物，具有碱性pH值的缓冲液的再吸附剂和用于检测果糖胺的显色剂。

    Process and reagent for the specific determination of HDL cholesterol in serum or plasma
    15.
    发明授权
    Process and reagent for the specific determination of HDL cholesterol in serum or plasma 失效
    用于血清或血浆中HDL胆固醇特异性测定的方法和试剂

    公开(公告)号：US4851335A

    公开(公告)日：1989-07-25

    申请号：US908031

    申请日：1986-09-16

    申请人： Lorenz Kerscher ,  Joachim Siedel ,  Joachim Ziegenhorn ,  Brigitte Pautz

    发明人： Lorenz Kerscher ,  Joachim Siedel ,  Joachim Ziegenhorn ,  Brigitte Pautz

    IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/60 ,  G01N33/92

    CPC分类号： C12Q1/60 ,  G01N33/92

    摘要： The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement.The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains0.1 to 10 U/ml. cholesterol esterase0.05 to 10 U/ml. cholesterol oxidase20 to 500 mmole/liter buffer substance (pH 6.0 to 8.0)0.2 to 20 mmole/liter of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate and, separately therefrom,0.02 to 2% non-ionic polyethylene oxide group-containing detergent or secondary alkane sulphonate, and optionally 0.05 to 2% of an alcohol containing up to 3 carbon atoms.

    摘要翻译： 本发明提供了通过与在缓冲的水性介质中含有胆固醇氧化酶和胆固醇酯酶的胆固醇检测系统温育并测量胆固醇氧化酶反应或胆固醇氧化酶反应产物的方法来特异性测定血清或血浆中的HDL胆固醇的方法 氧气消耗，其中将待测试样品在胆汁酸或胆汁酸衍生物或二辛基磺基琥珀酸盐的存在下孵育，然后进行第一次测量，随后进行非离子的含聚氧乙烯基团 加入洗涤剂或仲烷基磺酸盐，再次孵育并进行第二次测量，由第一次和第二次测量之间的差异确定HDL胆固醇的量。 本发明还提供了用于特异性测定含有胆固醇氧化酶，缓冲液和胆固醇酯酶的血清或血浆中的HDL胆固醇的试剂，其中称为最终溶液，其含有0.1至10U / ml。 胆固醇酯酶0.05〜10U / ml。 胆固醇氧化酶20-500毫摩尔/升缓冲物质（pH 6.0〜8.0）0.2〜20mmol /升胆汁酸或胆汁酸衍生物或二辛基磺基琥珀酸盐的盐，分别为0.02〜2％的非离子性 含有聚环氧乙烷的洗涤剂或仲烷烃磺酸盐，以及任选地0.05至2％的含有至多3个碳原子的醇。

    Process and reagent for the determination of creatinine
    16.
    发明授权
    Process and reagent for the determination of creatinine 失效
    用于测定肌酐的方法和试剂

    公开(公告)号：US4543325A

    公开(公告)日：1985-09-24

    申请号：US449925

    申请日：1982-12-15

    申请人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    发明人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    IPC分类号： G01N33/53 ,  G01N33/70 ,  C12N9/96 ,  C12Q1/34

    CPC分类号： G01N33/70 ,  Y10S435/81 ,  Y10S435/961 ,  Y10S436/822

    摘要： The present invention provides a process for the immunological determination of creatinine, wherein creatinine is converted into 1-methylhydantoin, the 1-methylhydantoin formed is incubated in an aqueous medium with antibodies which are directed against a conjugate of a first hydantoin derivative of the general formula: ##STR1## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance suitable for antibody formation, reacted with a conjugate of a second hydantoin derivative of general formula (I) with a second hapten carrier substance, one of the components antibody and conjugate being present in the solid phase or in dissolved form and the other component being present in dissolved form, and the inhibition of the binding reaction between the antibodies and the hydantoin conjugate with the second hapten carrier substance is measured.The present invention also provides a reagent for the immunological determination of creatinine, wherein it contains creatinine iminohydrolase, antibodies against a conjugate of a hydantoin of the general formula: ##STR2## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance, a conjugate of a hydantoin of general formula (I) with a second hapten carrier substance which does not cross-react substantially with the first hapten carrier substance and a buffer substance.

    摘要翻译： 本发明提供了一种免疫学测定肌酐的方法，其中将肌酐转化为1-甲基乙内酰脲，所形成的1-甲基乙内酰脲在水性介质中与针对通式 ：其中可以相同或不同的R 1，R 2，R 3和R 4为氢原子，含有至多3个碳原子的烷基或苯基，其中第一半抗原载体物质适用于抗体 与通式（I）的第二乙内酰脲衍生物与第二半抗原载体物质的缀合物反应，抗体和缀合物之一是固相或溶解形式存在，另一种成分以溶解形式存在 并且测量了与第二半抗原载体物质的抗体和乙内酰脲缀合物之间的结合反应的抑制。 本发明还提供了一种用于免疫测定肌酐的试剂，其中含有肌酐亚氨基水解酶，针对通式如下的乙内酰脲的缀合物的抗体：其中R1，R2，R3和R4可以相同 或不同的是氢原子，含有至多3个碳原子的烷基或苯基，具有第一半抗原载体物质，通式（I）的乙内酰脲与不会基本上交叉反应的第二半抗原载体物质的缀合物 具有第一半抗原载体物质和缓冲物质。

    Creatinine specific antibody
    17.
    发明授权
    Creatinine specific antibody 失效
    肌酸酐特异性抗体

    公开(公告)号：US4485177A

    公开(公告)日：1984-11-27

    申请号：US412411

    申请日：1982-08-27

    申请人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-George Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    发明人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-George Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

    IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K16/18 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/70 ,  G01N33/54 ,  A61K39/00 ,  C07G7/00

    CPC分类号： C07K16/18 ,  G01N33/531 ,  G01N33/54306 ,  G01N33/577 ,  G01N33/70 ,  A61K38/00

    摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.

    摘要翻译： 本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。 本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分 ，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。 此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

    Process for specific determination of cholesterol in the presence of the HDL fraction in serum
    20.
    发明授权
    Process for specific determination of cholesterol in the presence of the HDL fraction in serum 失效
    在血清中HDL级分存在下特异性测定胆固醇的方法

    公开(公告)号：US4544630A

    公开(公告)日：1985-10-01

    申请号：US468792

    申请日：1983-02-22

    申请人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

    发明人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

    IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/60

    CPC分类号： C12Q1/60

    摘要： The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0.The present invention also provides a reagent for carrying out this process.

    摘要翻译： 本发明提供了一种在胆固醇酯酶的作用下，通过胆固醇释放和胆固醇氧化酶氧化释放的胆固醇，在血清脂蛋白的HDL部分存在下，特异性测定LDL级分的胆固醇的方法 和氧气，形成过氧化氢和胆甾烯酮，动力学测量氧化酶反应的一种反应成分的变化，特别是过氧化氢的形成，其中测量在预定时间内进行，反应溶液 其表面活性剂浓度为0.01〜1.5mmol / l，胆固醇酯酶浓度为0.1〜30U / ml。 pH值为6.5〜8.0。 本发明还提供了一种用于进行该方法的试剂。