利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

38 条记录 (耗时 0.048 秒)

    Detection of specific sequences in nucleic acids
    12.
    发明授权
    Detection of specific sequences in nucleic acids 失效
    检测核酸中的特定序列

    公开(公告)号：US5521065A

    公开(公告)日：1996-05-28

    申请号：US257219

    申请日：1994-06-08

    申请人： Norman M. Whiteley ,  Michael W. Hunkapiller ,  Alexander N. Glazer

    发明人： Norman M. Whiteley ,  Michael W. Hunkapiller ,  Alexander N. Glazer

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6862 ,  C12Q1/6813 ,  C12Q1/6827 ,  Y10S435/803 ,  Y10S435/81

    摘要： A method of testing for the presence or absence of a target sequence in a mixture of single-stranded nucleic acid fragments is disclosed. The method involves reacting a mixture of single-stranded nucleic acid fragments with a first probe which is complementary to a first region of the target sequence, and with a second probe which is complementary to a second region of the target sequence, where the first and second target regions are contiguous with one another, under hybridization conditions in which the two probes become stably hybridized to their associated target regions. Following hybridization, any of the first and second probes hybridized to contiguous first and second target regions are ligated, and the sample is tested for the presence of expected probe ligation product. The presence of ligated product indicates that the target sequence is present in the sample.

    摘要翻译： 公开了在单链核酸片段的混合物中测试靶序列的存在或不存在的方法。 该方法包括使单链核酸片段的混合物与与靶序列的第一区域互补的第一探针和与靶序列的第二区域互补的第二探针反应，其中第一和 在杂交条件下，第二目标区域彼此连续，其中两个探针稳定地与其相关联的靶区域杂交。 杂交后，将与连续的第一和第二靶区域杂交的任何第一和第二探针连接，并测试样品中是否存在预期的探针连接产物。 连接产物的存在表明靶序列存在于样品中。

    Determination of peptide sequences
    14.
    发明授权
    Determination of peptide sequences 失效
    肽序列的测定

    公开(公告)号：US4852017A

    公开(公告)日：1989-07-25

    申请号：US65022

    申请日：1987-06-19

    申请人： Michael W. Hunkapiller

    发明人： Michael W. Hunkapiller

    IPC分类号： G01N33/50 ,  C07K1/12 ,  G01N33/68 ,  G06F17/00

    CPC分类号： G06K9/00503 ,  C07K1/128

    摘要： An apparatus and process are used to cyclicly degrade a peptide to be sequenced, arriving at a set of amino acid residues for each cycle. The amount of each amino acid residue is quantitatively measured in each set, then a background level is fit to each cycle to obtain a background fit. A measure of dispersion is then calculated for the background fit, and the measured amounts of amino acid residues in each cycle are normalized relative to the background fit. The largest normalized background-corrected residue amount in each cycle then provides a sequence assignment that can be used for further correction steps if desired.

    Quantitation of chromatographic information
    15.
    发明授权
    Quantitation of chromatographic information 失效
    色谱信息的定量

    公开(公告)号：US4837726A

    公开(公告)日：1989-06-06

    申请号：US65012

    申请日：1987-06-19

    申请人： Michael W. Hunkapiller

    发明人： Michael W. Hunkapiller

    IPC分类号： G01N30/86 ,  G01N30/88

    CPC分类号： G01N30/88 ,  G01N30/8641 ,  G01N2030/8804 ,  G01N2030/8831 ,  G01N30/8617 ,  G01N30/8631

    摘要： A process and apparatus for quantitating chromatographic information uses a discrete, linear, translation invariant filter function a.sub.N.sup..alpha., where N is a measure of the filter function width and .alpha. is a parameter whose value determines signal to noise characteristics of the filter function. First a chromatographic analysis of a sample is performed to obtain a first chromatogram. Then the first chromatogram is filtered with the filter function, with N set to approximate the width of peaks obtained in the first chromatogram, and .alpha. is set to filter out high frequency noise from the first chromatogram to obtain a second chromatogram having a first filtered baseline. The second chromatogram is then filtered with .alpha. set to resolution enhance peaks in the second chromatogram to obtain a third chromatogram having a baseline which is substantially the same as the first filtered baseline. The second chromatogram is then subtracted from the third chromatogram to obtain a fourth chromatogram which is baseline corrected.

    Apparatus for the performance of chemical processes
    17.
    发明授权
    Apparatus for the performance of chemical processes 失效
    用于化学工艺性能的设备

    公开(公告)号：US4252769A

    公开(公告)日：1981-02-24

    申请号：US106828

    申请日：1979-12-26

    申请人： Leroy E. Hood ,  Michael W. Hunkapiller

    发明人： Leroy E. Hood ,  Michael W. Hunkapiller

    IPC分类号： B01J19/00 ,  C40B40/06 ,  C40B60/14 ,  G01N33/483 ,  G01N35/00 ,  G01N35/10 ,  G01N1/18 ,  G01N9/30

    CPC分类号： G01N35/00 ,  B01J19/0046 ,  B01J2219/00421 ,  B01J2219/0049 ,  B01J2219/00695 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  G01N35/1079

    摘要： An improved apparatus for the performance of chemical processes having a reaction chamber with an open end, a reaction cell mounted within the chamber and having a substantially cylindrical interior wall in which is formed an annular groove, and means for spinning the cell about its axis to distribute a chemical compound on the interior wall as a thin film, which includes a closure member mounted for rotation about an axis displaced from the axis of the cell, and a fluid conduit extending through the closure member and terminating at an inner end within the cell for the withdrawal of fluid therefrom. The inner end of the conduit is displaced from the axis of rotation of the closure member and is positioned substantially opposite the annular groove such that it is actuable for adjustment between a first location displaced radially from the annular groove and a second location substantially within the groove by rotation of the closure member through a preselected angle.

    摘要翻译： 一种用于执行具有开口端的反应室的化学方法的改进装置，安装在室内的反应池，并具有基本上圆柱形的内壁，其中形成有环形槽，以及用于将电池绕其轴线旋转的装置 在内壁上分布化学化合物作为薄膜，其包括安装成围绕从电池轴线移动的轴线旋转的封闭构件以及延伸穿过封闭构件并终止于电池内部的内端的流体导管 用于从中排出流体。 导管的内端从闭合构件的旋转轴线移位并且基本上与环形槽相对地定位，使得其可致动以在从环形槽径向偏移的第一位置和基本上位于凹槽内的第二位置之间进行调节 通过关闭构件的旋转预定角度。

    BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION
    18.
    发明申请
    BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION 有权
    二次探针和夹钳组合物和方法进行目标杂交检测

    公开(公告)号：US20100291557A1

    公开(公告)日：2010-11-18

    申请号：US12538823

    申请日：2009-08-10

    申请人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    发明人： Kenneth J. Livak ,  Michael Egholm ,  Michael W. Hunkapiller

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2549/126 ,  C12Q2537/119 ,  C12Q2525/179

    摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

    摘要翻译： 二进制探针和夹具组合物进行靶杂交检测的方法。 当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。 当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。 探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。 夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。

    Methods and kits for methylation detection
    20.
    发明授权
    Methods and kits for methylation detection 有权
    用于甲基化检测的方法和试剂盒

    公开(公告)号：US07364855B2

    公开(公告)日：2008-04-29

    申请号：US11119985

    申请日：2005-05-02

    申请人： Mark R. Andersen ,  Jer-Kang Chen ,  Michael W. Hunkapiller ,  Steven M. Menchen

    发明人： Mark R. Andersen ,  Jer-Kang Chen ,  Michael W. Hunkapiller ,  Steven M. Menchen

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6862 ,  C12Q2537/164 ,  C12Q2521/501

    摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.

    摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。