公开(公告)号：US5871949A

公开(公告)日：1999-02-16

申请号：US980792

申请日：1997-12-01

申请人： Hiroyuki Ebinuma ,  Koji Ushizawa

发明人： Hiroyuki Ebinuma ,  Koji Ushizawa

IPC分类号： C12Q1/32 ,  C12Q1/00 ,  C12Q1/54

CPC分类号： C12Q1/32

摘要： Using a 1,5-anhydroglucitol dehydrogenase capable of acting on 1,5-anhydroglucitol and directly catalyzing a reducing chromophoric agent in the absence of an electron carrier, the 1,5-anhydroglucitol dehydrogenase is allowed to act on 1,5-anhydroglucitol in the presence of the reducing chromophoric agent preferably after the glucose in the specimen has been changed, or while being changed, into such a structure that it does not react with the 1,5-anhydroglucitol dehydrogenase in the specimen, by the aid of a glucose eliminator. Then, the amount of the resultant reduced colored substance is measured. As the 1,5-anhydroglucitol dehydrogenase, an enzyme produced by a microorganism having the ability to produce the 1,5-anhydroglucitol dehydrogenase is preferably used.

摘要翻译： 使用能够作用于1,5-脱水葡萄糖醇并在不存在电子载体的情况下直接催化还原性发色剂的1,5-脱水葡萄糖醇脱氢酶，使1,5-脱水葡萄糖醇脱氢酶作用于1,5-脱水葡萄糖醇 还原性发色剂的存在优选在样品中的葡萄糖已经被改变或改变之后，使其不与样品中的1,5-脱水葡萄糖醇脱氢酶反应，借助于葡萄糖 消除者 然后，测量所得的还原着色物质的量。 作为1,5-脱水葡萄糖醇脱氢酶，优选使用具有生产1,5-脱水葡萄糖醇脱氢酶能力的微生物产生的酶。

公开(公告)号：US20140147842A1

公开(公告)日：2014-05-29

申请号：US13979256

申请日：2012-01-12

申请人： Takuya Yotani ,  Eiji Kiyotoh ,  Koji Ushizawa

发明人： Takuya Yotani ,  Eiji Kiyotoh ,  Koji Ushizawa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q1/6858 ,  C12Q2565/137

摘要： An object of the present invention is to provide a method for rapidly and simply detecting single nucleotide polymorphisms. The present invention is a method for detecting single nucleotide polymorphisms, comprising analyzing wild-type and mutant-type products amplified by an AS-PCR method using ion-exchange chromatography.

摘要翻译： 本发明的目的是提供一种快速简单地检测单核苷酸多态性的方法。 本发明是用于检测单核苷酸多态性的方法，其特征在于，包括分析通过使用离子交换色谱法的AS-PCR方法扩增的野生型和突变型产物。

公开(公告)号：US06969613B1

公开(公告)日：2005-11-29

申请号：US09856790

申请日：1999-12-07

申请人： Hiroyuki Ebinuma ,  Koji Ushizawa

发明人： Hiroyuki Ebinuma ,  Koji Ushizawa

IPC分类号： C12Q1/527 ,  G01N31/22 ,  G01N33/00

CPC分类号： C12Q1/527 ,  G01N31/22 ,  G01N31/224 ,  G01N33/0044 ,  Y10T436/18 ,  Y10T436/182

摘要： The present invention provides a method for quantitatively determining hydrogen sulfide or sulfide ions conveniently with high sensitivity, which comprises adding to a sample containing hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator. The present invention further provides a method for quantitatively determining a specific substance, which comprises adding to a sample containing a specific substance, a component which acts on the specific substance so that the specific substance forms hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions, and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator.

摘要翻译： 本发明提供了一种以高灵敏度方便地定量测定硫化氢或硫化物离子的方法，其包括向含有硫化氢或硫化物离子的金属离子或释放所述金属离子的化合物和与 金属离子发生显色，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。 本发明还提供了一种定量测定特定物质的方法，该方法包括向含有特定物质的样品中加入作用于特定物质的成分，使特定物质形成硫化氢或硫化物离子，金属离子或化合物 释放出所述金属离子的金属指示剂和与金属离子反应并导致发色的金属指示剂，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。

公开(公告)号：US06632677B1

公开(公告)日：2003-10-14

申请号：US09786749

申请日：2001-03-09

申请人： Masahiro Sekiguchi ,  Toshikatsu Abe ,  Koji Ushizawa

发明人： Masahiro Sekiguchi ,  Toshikatsu Abe ,  Koji Ushizawa

IPC分类号： G01N3100

CPC分类号： B01J19/16 ,  C08K5/01 ,  G01N2035/00277 ,  G01N2035/00287 ,  Y02P20/149 ,  Y10T436/10 ,  Y10T436/108331 ,  Y10T436/21 ,  Y10T436/214 ,  Y10T436/25 ,  Y10T436/2525 ,  C08L83/04

摘要： An air-barrier agent for an aqueous reagent or an aqueous specimen having as an effective component a mixture of a chain hydrocarbon and a silicone oil immiscible with the aqueous reagent as well methods of using and making the same.

摘要翻译： 作为水性试剂的空气阻隔剂或具有与含水试剂不混溶的链烃和硅油的混合物作为有效成分的水性试剂，以及使用和制造其的方法。

公开(公告)号：US4889801A

公开(公告)日：1989-12-26

申请号：US63912

申请日：1987-06-19

申请人： Koji Ushizawa

发明人： Koji Ushizawa

IPC分类号： C12N9/04 ,  C12Q1/26 ,  C12R1/38

CPC分类号： C12N9/0006 ,  C12Q1/26

摘要： Disclosed herein is a 3.alpha.-hydroxysteroid oxidase which oxidizes a 3.alpha.-hydroxysteroid into a 3-oxosteroid and hydrogen peroxide. The oxidase may preferably be obtained from a culture broth of Pseudomonas testosteroni (ATCC 11996). The oxidase is useful for the quantitative analysis of a 3.alpha.-hydroxysteroid. Method and reagent useful for the quantitative analysis of a 3.alpha.-hydroxysteroid are also disclosed.

摘要翻译： 本文公开了3α-羟基类固醇氧化酶，其将3α-羟基固醇氧化成3-氧代甾族化合物和过氧化氢。 氧化酶可以优选地从睾丸素假单胞菌（ATCC 11996）的培养液中获得。 氧化酶可用于3α-羟基类固醇的定量分析。 还公开了可用于3α-羟基类固醇的定量分析的方法和试剂。

公开(公告)号：US4816394A

公开(公告)日：1989-03-28

申请号：US861725

申请日：1986-05-12

申请人： Koji Ushizawa ,  Akemichi Maki ,  Toshiyuki Akimoto ,  Senkichi Nagasaki ,  Miyoshi Hirata

发明人： Koji Ushizawa ,  Akemichi Maki ,  Toshiyuki Akimoto ,  Senkichi Nagasaki ,  Miyoshi Hirata

IPC分类号： G01N33/74 ,  C12Q1/26 ,  C12Q1/32

CPC分类号： C12Q1/32

摘要： A 3.alpha.-hydroxysteroid contained in a sample can be quantitatively analyzed by causing a 3.alpha.-hydroxysteroid dehydrogenase to act on the sample in the presence of nicotinamide adenine dinucleotide, causing tetrazolium or a salt thereof, an electron carrier substance, and 3-oxosteroid-.DELTA..sup.1 -dihydrogenase and/or 3-oxosteroid-.DELTA..sup.4 -dehydrogenase to act on the thus-obtained reaction product, and then quantitatively analyzing the resultant formazan.

摘要翻译： 通过使3α-羟基类固醇脱氢酶在烟酰胺腺嘌呤二核苷酸存在下作用于样品，引起四唑或其盐，电子载体物质和3-氧化甾体，可以定量分析样品中含有的3α-羟基固醇类 - DELTA 1-二氢化酶和/或3-氧代甾体-DTA4-脱氢酶作用于由此获得的反应产物，然后定量分析所得的甲an。

公开(公告)号：US09481881B2

公开(公告)日：2016-11-01

申请号：US13979241

申请日：2012-01-12

申请人： Takuya Yotani ,  Koji Ushizawa

发明人： Takuya Yotani ,  Koji Ushizawa

IPC分类号： C07H21/00 ,  C12Q1/68 ,  B01D15/00 ,  C12N15/10 ,  G01N30/96 ,  G01N30/02

CPC分类号： C12N15/101 ,  C12Q1/6806 ,  G01N30/96 ,  Y10T436/143333

摘要： The present invention provides eluent for ion-exchange chromatography, wherein the eluent allows separation and detection of a target nucleic acid such as a PCR-amplified product, a restriction enzyme fragment of the PCR-amplified product, or a restriction enzyme fragment of a nucleic acid in a short time with high separation performance. The present invention also provides a method of analyzing nucleic acid chains by ion-exchange chromatography using the eluent. The present invention provides an eluent for ion-exchange chromatography comprising a guanidine salt derived from guanidine represented by the following formula (1):

公开(公告)号：US20130330735A1

公开(公告)日：2013-12-12

申请号：US13979241

申请日：2012-01-12

申请人： Takuya Yotani ,  Koji Ushizawa

发明人： Takuya Yotani ,  Koji Ushizawa

IPC分类号： G01N30/96

CPC分类号： C12N15/101 ,  C12Q1/6806 ,  G01N30/96 ,  Y10T436/143333

摘要： The present invention provides eluent for ion-exchange chromatography, wherein the eluent allows separation and detection of a target nucleic acid such as a PCR-amplified product, a restriction enzyme fragment of the PCR-amplified product, or a restriction enzyme fragment of a nucleic acid in a short time with high separation performance. The present invention also provides a method of analyzing nucleic acid chains by ion-exchange chromatography using the eluent. The present invention provides an eluent for ion-exchange chromatography comprising a guanidine salt derived from guanidine represented by the following formula (1):

摘要翻译： 本发明提供了用于离子交换层析的洗脱液，其中洗脱液允许分离和检测目标核酸，例如PCR扩增产物，PCR扩增产物的限制酶片段或核酸的限制酶片段 酸在短时间内具有高分离性能。 本发明还提供了使用洗脱液通过离子交换层析分析核酸链的方法。 本发明提供了一种用于离子交换层析的洗脱液，其包含由下式（1）表示的胍衍生的胍盐：

公开(公告)号：US06541215B1

公开(公告)日：2003-04-01

申请号：US09708454

申请日：2000-11-09

申请人： Hiroyuki Ebinuma ,  Koji Ushizawa

发明人： Hiroyuki Ebinuma ,  Koji Ushizawa

IPC分类号： C12Q126

CPC分类号： C12Q1/32 ,  Y10S435/823 ,  Y10S435/962

摘要： Disclosed are a method for quantitatively determining mannose, which comprises reacting mannose in a specimen with an enzyme which is capable of oxidizing the mannose by dehydrogenation, in the presence of an electron acceptor, and quantitatively determining a formed reductant of the electron acceptor; and a reagent for the quantitative determination of mannose.

摘要翻译： 本发明公开了一种定量测定甘露糖的方法，其包括使试样中的甘露糖与能够通过脱氢氧化甘露糖的酶在电子受体存在下反应并定量测定形成的电子受体的还原剂; 和用于定量测定甘露糖的试剂。

公开(公告)号：US5843677A

公开(公告)日：1998-12-01

申请号：US722153

申请日：1996-10-18

申请人： Yoshiro Niitsu ,  Yutaka Kohgo ,  Junji Kato ,  Hirokazu Yago ,  Hisashi Hanada ,  Koji Ushizawa

发明人： Yoshiro Niitsu ,  Yutaka Kohgo ,  Junji Kato ,  Hirokazu Yago ,  Hisashi Hanada ,  Koji Ushizawa

IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/68 ,  C07K16/28

CPC分类号： G01N33/68

摘要： The present invention is directed to a method of immunoassay of asialoglycoprotein receptors (AGPR) by bringing a specimen into contact with a monoclonal antibody that recognizes AGPR, wherein the pH of a diluted specimen solution is adjusted from 5 to 7 or phenol is added to a solution of an enzyme-labeled antibody. This method is excellent in the sensitivity and accuracy of AGPR assay and can be applied also to the screening of hepatopathy remedies.

摘要翻译： PCT No.PCT / JP95 / 00770 Sec。 371日期：1996年10月18日 102（e）日期1996年10月18日PCT提交1995年4月19日PCT公布。 WO95 / 29403 PCT出版物 日期：1995年11月2日本发明涉及一种通过使样品与识别AGPR的单克隆抗体接触的免疫测定来测定脱唾液酸糖蛋白受体（AGPR）的方法，其中将稀释样品溶液的pH调节为5〜7或 将苯酚加入到酶标记抗体的溶液中。 该方法在AGPR测定的灵敏度和准确性方面优异，也可用于筛查肝病治疗。