公开(公告)号：US20140073756A1

公开(公告)日：2014-03-13

申请号：US14081224

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Nini H. KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang Ying ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C07F7/18 ,  C08F236/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合 形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。

公开(公告)号：US20200179541A1

公开(公告)日：2020-06-11

申请号：US16713388

申请日：2019-12-13

Applicant: Life Technologies Corporation

Inventor： Brian AGNEW ,  Robert AGGELER ,  Hee Chol KANG ,  Aimei CHEN ,  Kyle GEE

IPC: A61K51/10 ,  G01N33/534 ,  G01N33/68 ,  C07K14/435 ,  C07K16/18 ,  C12P21/00 ,  C12P21/06 ,  G01N33/58

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

公开(公告)号：US20180222855A1

公开(公告)日：2018-08-09

申请号：US15894057

申请日：2018-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  C07D311/84 ,  C07C309/51 ,  G01N33/58

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20160031821A1

公开(公告)日：2016-02-04

申请号：US14810766

申请日：2015-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Aimei CHEN ,  Hee Chol KANG

IPC: C07D225/08 ,  C07D225/02 ,  C07C309/42

CPC classification number: C07D225/08 ,  C07C309/42 ,  C07C2603/36 ,  C07D223/08 ,  C07D223/26 ,  C07D225/02 ,  C07K1/13 ,  C07K16/18 ,  G01N33/532

Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.

Abstract translation: 本公开涉及用于将点击反应性基团与载体分子或固体支持物缀合的反应性酯试剂。 反应性酯剂具有通式IA，其中在整个申请中描述了变量R1，R2，R3，Ra和L。

公开(公告)号：US20160010145A1

公开(公告)日：2016-01-14

申请号：US14649370

申请日：2013-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason DALLWIG ,  Kyle GEE ,  Nancy AHNERT ,  Hee Chol KANG ,  Jongtae YANG

IPC: C12Q1/68 ,  C09B23/00

CPC classification number: C12Q1/6825 ,  C09B23/00 ,  C09B23/04 ,  C09B23/06 ,  C09B23/083 ,  C09B23/086 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q2525/207 ,  C12Q2563/107 ,  C12Q2563/173

Abstract: Substituted unsymmetrical cyanine dyes are provided herein, including kits, assays systems and methods for their use in the detection of RNA compounds (e.g., miRNA).

Abstract translation: 本文提供取代的不对称花青染料，包括用于检测RNA化合物（例如miRNA）的试剂盒，测定系统和方法。