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    System and method for determining copies-per-unit-volume using PCR and flow control of droplets
    11.
    发明授权
    System and method for determining copies-per-unit-volume using PCR and flow control of droplets 有权

    公开(公告)号：US10557174B2

    公开(公告)日：2020-02-11

    申请号：US15080801

    申请日：2016-03-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael C. Pallas ,  Marcin Sikora ,  Casey R. McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

    IPC: C12Q1/6886 ,  C12Q1/686 ,  G01N21/64

    Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    12.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    多重PCR的方法和组合

    公开(公告)号：US20150322490A1

    公开(公告)日：2015-11-12

    申请号：US14796400

    申请日：2015-07-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark Andersen ,  Michael Thornton

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    Abstract translation: 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 特别地，提供允许一个或多个靶序列的选择性扩增的各种靶特异性引物。 一方面，本发明涉及可用于选择性扩增与癌症或遗传性疾病相关的一种或多种靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增靶序列可用于包括核酸测序在内的各种下游过程，并用于检测遗传变体的存在。

    Compositions, methods, systems and kits for target nucleic acid enrichment
    13.
    发明授权
    Compositions, methods, systems and kits for target nucleic acid enrichment 有权
    用于靶核酸浓缩的组合物，方法，系统和试剂盒

    公开(公告)号：US09133510B2

    公开(公告)日：2015-09-15

    申请号：US14054618

    申请日：2013-10-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Steven Roman

    IPC: C07H21/00 ,  C12Q1/68

    CPC classification number: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2563/131 ,  C12Q2563/149

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

    Abstract translation: 本发明提供了可用于从样品中分离核酸分子的方法，组合物，试剂盒，系统和装置。 特别地，所述方法通常涉及从样品归一化靶核酸分子的浓度。 一方面，本发明涉及从引物延伸反应混合物中纯化引物延伸产物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的核酸分子可以用于包括核酸测序在内的各种下游过程。

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    14.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 有权

    公开(公告)号：US20250011760A1

    公开(公告)日：2025-01-09

    申请号：US18768211

    申请日：2024-07-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Sihong Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Guobin Luo

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B20/04 ,  C40B40/06 ,  C40B50/06

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    Compositions, methods, systems and kits for target nucleic acid enrichment
    15.
    发明授权
    Compositions, methods, systems and kits for target nucleic acid enrichment 有权

    公开(公告)号：US11130984B2

    公开(公告)日：2021-09-28

    申请号：US16846932

    申请日：2020-04-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Steven Roman

    IPC: C12Q1/6806 ,  C12Q1/6853 ,  C07H21/00

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

    Methods and compositions for multiplex PCR
    16.
    发明授权
    Methods and compositions for multiplex PCR 有权

    公开(公告)号：US10266881B2

    公开(公告)日：2019-04-23

    申请号：US14796400

    申请日：2015-07-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John Leamon ,  Mark Andersen ,  Michael Thornton

    IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6806 ,  C07H21/04

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    SYSTEM AND METHOD FOR DETERMINING COPIES-PER-UNIT-VOLUME USING PCR AND FLOW CONTROL OF DROPLETS
    20.
    发明申请
    SYSTEM AND METHOD FOR DETERMINING COPIES-PER-UNIT-VOLUME USING PCR AND FLOW CONTROL OF DROPLETS 审中-公开
    使用PCR和流量控制法确定复制单位体积的系统和方法

    公开(公告)号：US20160208342A1

    公开(公告)日：2016-07-21

    申请号：US15080801

    申请日：2016-03-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael C. Pallas ,  Marcin Sikora ,  Casey R. McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

    IPC: C12Q1/68 ,  G01N21/64

    CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N21/6486 ,  C12Q2537/16 ,  C12Q2563/143 ,  C12Q2563/159 ,  C12Q2565/626

    Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

    Abstract translation: 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。

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