公开(公告)号：US20190264272A1

公开(公告)日：2019-08-29

申请号：US16351194

申请日：2019-03-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Bin LI ,  Kai Lao ,  Jennifer O'NEIL ,  Jennifer KUNKEL ,  Kellie HALEY ,  Rachel KASINSKAS ,  Zhaochun MA ,  Pius BRZOSKA

IPC: C12Q1/6853 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q1/46 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12P19/34

Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

公开(公告)号：US20180237830A1

公开(公告)日：2018-08-23

申请号：US15891454

申请日：2018-02-08

Applicant: Life Technologies Corporation

Inventor： Yongmei (Alexis) JI ,  Pius BRZOSKA ,  Angela BURRELL ,  Craig CUMMINGS ,  Catherine O'CONNELL

IPC: C12Q1/689 ,  C12Q1/6806

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US20240392394A1

公开(公告)日：2024-11-28

申请号：US18262672

申请日：2022-01-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Pius BRZOSKA ,  Joyce WILDE ,  Manohar FURTADO ,  Sara KENDRICK ,  Camilla ULEKLEIV ,  Junko STEVENS ,  Kamini VARMA ,  Kathleen HAYASHIBARA

IPC: C12Q1/70

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US20210254181A1

公开(公告)日：2021-08-19

申请号：US17301976

申请日：2021-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6851

Abstract: A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.

公开(公告)号：US20210032691A1

公开(公告)日：2021-02-04

申请号：US16949083

申请日：2020-10-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Bin LI ,  Kai Qin LAO ,  Jennifer O'NEIL ,  Jennifer KUNKEL ,  Kellie HALEY ,  Rachel KASINSKAS ,  Zhaochun MA ,  Pius BRZOSKA

IPC: C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12P19/34 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/46

Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

公开(公告)号：US20200095626A1

公开(公告)日：2020-03-26

申请号：US16706394

申请日：2019-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei (Alexis) JI ,  Pius BRZOSKA ,  Angela BURRELL ,  Craig CUMMINGS ,  Catherine O'CONNELL

IPC: C12Q1/689 ,  C12Q1/6806

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US20170362640A1

公开(公告)日：2017-12-21

申请号：US15625550

申请日：2017-06-16

Applicant: Life Technologies Corporation

Inventor： Ioanna PAGANI ,  Pius BRZOSKA ,  Kelly LI ,  Sunali PATEL ,  Boli HUANG ,  Kamini VARMA ,  Nitin PURI ,  Evan DIAMOND

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6888 ,  C12Q1/6895 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.

公开(公告)号：US20170096711A1

公开(公告)日：2017-04-06

申请号：US15128076

申请日：2015-03-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Q2537/16 ,  C12Q2543/10 ,  C12Q2545/101 ,  C12Q2547/107 ,  C12Q2561/101

Abstract: A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.