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    Compositions and methods for detection of microorganisms of the Mycobacterium avium complex excluding Mycobacterium avium paratuberculosis
    11.
    发明授权
    Compositions and methods for detection of microorganisms of the Mycobacterium avium complex excluding Mycobacterium avium paratuberculosis 有权
    用于检测鸟分枝杆菌复合物微生物的组合物和方法，不包括副结核分枝杆菌

    公开(公告)号：US09334542B2

    公开(公告)日：2016-05-10

    申请号：US13785531

    申请日：2013-03-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Craig Cummings ,  Pius Brzoska ,  Angela Burrell ,  Catherine O'Connell

    IPC: C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/112

    Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of a non-Mycobacterium avium subsp. paratuberculosis (non-MAP) organism, wherein a non-MAP organism is a Mycobacterium avium complex (MAC) organism that does not belong to the Mycobacterium avium subsp. paratuberculosis (MAP) organism, from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample. Exemplary non-MAP organisms including M. avium subsp. avium (MAA), M. avium subsp. hominissuis (MAH), and M. avium subsp. silvaticum (MAS) can be detected by the present compositions, kits and methods.

    Abstract translation: 公开了用于特异性和差异性检测非分枝杆菌鸟属亚种的组合物，测定，方法，诊断方法，试剂盒和诊断试剂盒。 副结核病（非MAP）生物体，其中非MAP生物体是不属于鸟分枝杆菌亚种的鸟分枝杆菌复合体（MAC）生物。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（如土壤，污垢，垃圾，污水，空气或水）在内的样品的副结核病（MAP）生物，包括食品加工和制造表面，或 生物样品。 示例性非MAP生物体，包括鸟分枝杆菌 鸟类（MAA），鸟分枝杆菌 山楂（MAH）和鸟分枝杆菌 可以通过本发明的组合物，试剂盒和方法检测silvaticum（MAS）。

    E. COLI O157:H7 SPECIFIC ASSAY
    12.
    发明申请
    E. COLI O157:H7 SPECIFIC ASSAY 有权
    大肠杆菌O157：H7特异性测定

    公开(公告)号：US20130236896A1

    公开(公告)日：2013-09-12

    申请号：US13870779

    申请日：2013-04-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Paolo VATTA ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/142

    Abstract: Disclosed are assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from complex food matrices, water, a beverage sample, a fermentation broth, a forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

    Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而非大肠杆菌O55：H7的分析，方法和试剂盒，来自复杂的食物基质，水，饮料样品，发酵液，法医样品，环境样品（例如 ，土壤，污垢，垃圾，污水，空气或水），包括食品加工和制造表面，或生物样品。

    Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
    16.
    发明授权
    Methods, compositions, systems, apparatuses and kits for nucleic acid amplification 有权
    用于核酸扩增的方法，组合物，系统，装置和试剂盒

    公开(公告)号：US09309566B2

    公开(公告)日：2016-04-12

    申请号：US13828049

    申请日：2013-03-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Bin Li ,  Kai Qin Lao ,  Jennifer O'Neil ,  Jennifer Kunkel ,  Kellie Haley ,  Rachel Kasinskas ,  Zhaochun Ma ,  Pius Brzoska

    IPC: C12Q1/68

    CPC classification number: C12Q1/6853 ,  C12Q1/6874 ,  C12Q2521/531 ,  C12Q2525/117 ,  C12Q2535/119 ,  C12Q2523/313

    Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.

    Abstract translation: 在一些实施方案中，本教导提供了用于配对末端测序的方法。 在一些实施方案中，待进行配对末端测序的多核苷酸模板包含至少一个交联部分和至少一个可剪切部分。 在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）切割步骤，和（c）反向测序步骤。 在一些实施方案中，配对末端测序反应包括（a）正向测序步骤，（b）交联步骤，（c）切割步骤和（d）反向测序步骤。

    Multi-primer Assay for Mycoplasma Detection
    17.
    发明申请
    Multi-primer Assay for Mycoplasma Detection 有权
    用于支原体检测的多引物测定

    公开(公告)号：US20140378330A1

    公开(公告)日：2014-12-25

    申请号：US14314985

    申请日：2014-06-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Olga PETRAUSKENE ,  Pius Brzoska ,  Somaya Bit ,  Jen-Kuei Liu ,  Robert Tebbs ,  Manohar Furtado

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

    Abstract translation: 公开了一种多引物扩增测定法，用于利用在单个容器中与样品接触的多个寡核苷酸引物检测支原体物种和密切相关物种的检测扩增产物的方法和试剂盒，其中扩增产物的存在表示支原体 在样品中。

    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS
    18.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
    用于检测多种微生物的组合物和方法

    公开(公告)号：US20140005061A1

    公开(公告)日：2014-01-02

    申请号：US13790447

    申请日：2013-03-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Robert TEBBS ,  Craig Cummings ,  Pius Brzoska ,  Sharon Matheny ,  Catherine O'Connell ,  Manohar Furtado ,  Nikolay Sergeev ,  Maxim Brevnov

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.

    Abstract translation: 本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。 一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。 还描述了用于多个微生物检测和鉴定的工作流程。

    COMPOSITIONS AND METHODS FOR DETECTION OF MICROORGANISMS OF THE MYCOBACTERIUM AVIUM COMPLEX EXCLUDING MYCOBACTERIUM AVIUM PARATUBERCULOSIS
    19.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF MICROORGANISMS OF THE MYCOBACTERIUM AVIUM COMPLEX EXCLUDING MYCOBACTERIUM AVIUM PARATUBERCULOSIS 有权
    用于检测MYCOBACTERIUM AVIUM复合物微生物的组合物和方法，不包括MYCOBACTERIUM AVIUM PARATUBERCULOSIS

    公开(公告)号：US20130260375A1

    公开(公告)日：2013-10-03

    申请号：US13785531

    申请日：2013-03-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Craig CUMMINGS ,  Pius Brzoska ,  Angela Burrell ,  Catherine O'Connell

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/112

    Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of a non-Mycobacterium avium subsp. paratuberculosis (non-MAP) organism, wherein a non-MAP organism is a Mycobacterium avium complex (MAC) organism that does not belong to the Mycobacterium avium subsp. paratuberculosis (MAP) organism, from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample. Exemplary non-MAP organisms including M. avium subsp. avium (MAA), M. avium subsp. hominissuis (MAH), and M. avium subsp. silvaticum (MAS) can be detected by the present compositions, kits and methods.

    Abstract translation: 公开了用于特异性和差异性检测非分枝杆菌鸟属亚种的组合物，测定，方法，诊断方法，试剂盒和诊断试剂盒。 副结核病（非MAP）生物体，其中非MAP生物体是不属于鸟分枝杆菌亚种的鸟分枝杆菌复合体（MAC）生物。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（如土壤，污垢，垃圾，污水，空气或水）在内的样品的副结核病（MAP）生物，包括食品加工和制造表面，或 生物样品。 示例性非MAP生物体，包括鸟分枝杆菌 鸟类（MAA），鸟分枝杆菌 山楂（MAH）和鸟分枝杆菌 可以通过本发明的组合物，试剂盒和方法检测silvaticum（MAS）。

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