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    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES
    11.
    发明申请
    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES 有权

    公开(公告)号：US20210214787A1

    公开(公告)日：2021-07-15

    申请号：US17217492

    申请日：2021-03-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Guobin LUO ,  Marina SEDOVA ,  David LIGHT ,  Ryan JONES ,  Mohammad ALANJARY

    IPC: C12Q1/6874 ,  C12Q1/6806

    Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.

    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    12.
    发明申请
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20190270974A1

    公开(公告)日：2019-09-05

    申请号：US16415063

    申请日：2019-05-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
    13.
    发明申请
    POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME 审中-公开

    公开(公告)号：US20170096648A1

    公开(公告)日：2017-04-06

    申请号：US15277834

    申请日：2016-09-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： DANIEL MAZUR ,  Peter VANDER HORN ,  Eileen TOZER ,  Sihong CHEN ,  Guobin LUO ,  Joshua SHIRLEY ,  Kevin HEINEMANN

    IPC: C12N9/12

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION
    14.
    发明申请
    METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION 有权
    使用能量转移检测的单分子序列的方法和装置

    公开(公告)号：US20150118679A1

    公开(公告)日：2015-04-30

    申请号：US14584829

    申请日：2014-12-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Joseph BEECHEM ,  Theo NIKIFOROV ,  Vi-En CHOONG ,  Xinzhan PENG ,  Guobin LUO ,  Cheng-Yao CHEN ,  Michael PREVITE

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

    Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

    Abstract translation: 本文提供了用于核苷酸掺入反应的系统和方法。 该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。 当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。 当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。 检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。

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