Method for testing a substance which is potentially active in the field of lipolysis and its mainly cosmetic use
    14.
    发明授权
    Method for testing a substance which is potentially active in the field of lipolysis and its mainly cosmetic use 失效
    用于测试在脂肪分解领域潜在活性物质的方法,其主要是化妆品用途

    公开(公告)号:US08741359B2

    公开(公告)日:2014-06-03

    申请号:US13536342

    申请日:2012-06-28

    IPC分类号: A01N65/00

    摘要: A method of testing a substance potentially active in the field of lipolysis comprising: preparing a substrate containing at least one triacylglycerol; placing this substrate in contact with a substance potentially active in the field of lipolysis, and with a lipoprotein lipase, in the presence of a cofactor of lipoprotein lipase, for a period of time sufficient to release at least in part one fatty acid of the triacylglycerol; and determining the capacity of inhibition of the release of the fatty acid resulting from the activity of the lipoprotein lipase, under the action of said potentially active substance, and evaluating the result of the inhibition which is either compared to the result obtained in the absence of the potentially active substance tested or compared with the result obtained in the presence of a known inhibitor acting as reference. Cosmetic and pharmaceutical uses are described.

    摘要翻译: 一种测试在脂肪分解领域潜在有活性的物质的方法,包括:制备含有至少一种三酰基甘油的底物; 将该底物置于与脂解作用中潜在有活性的物质接触,并在脂蛋白脂肪酶辅助因子存在下与脂蛋白脂肪酶接触足以释放三酰基甘油的至少一部分脂肪酸的时间 ; 并确定在所述潜在活性物质的作用下抑制由脂蛋白脂肪酶的活性引起的脂肪酸的释放的能力,并评估抑制结果,其与在不存在 所测定的潜在活性物质与作为参考的已知抑制剂存在下获得的结果进行比较。 描述了化妆品和药物用途。

    Method for Testing a Substance Which is Potentially Active in the Field of Lipolysis and its Mainly Cosmetic Use
    17.
    发明申请
    Method for Testing a Substance Which is Potentially Active in the Field of Lipolysis and its Mainly Cosmetic Use 失效
    在脂肪分解领域潜在活性物质的测试方法及其主要化妆品用途

    公开(公告)号:US20120269910A1

    公开(公告)日:2012-10-25

    申请号:US13536342

    申请日:2012-06-28

    摘要: A method of testing a substance potentially active in the field of lipolysis comprising: preparing a substrate containing at least one triacylglycerol; placing this substrate in contact with a substance potentially active in the field of lipolysis, and with a lipoprotein lipase, in the presence of a cofactor of lipoprotein lipase, for a period of time sufficient to release at least in part one fatty acid of the triacylglycerol; and determining the capacity of inhibition of the release of the fatty acid resulting from the activity of the lipoprotein lipase, under the action of said potentially active substance, and evaluating the result of the inhibition which is either compared to the result obtained in the absence of the potentially active substance tested or compared with the result obtained in the presence of a known inhibitor acting as reference. Cosmetic and pharmaceutical uses are described.

    摘要翻译: 一种测试在脂肪分解领域潜在有活性的物质的方法,包括:制备含有至少一种三酰基甘油的底物; 将该底物置于与脂解作用潜在活性的物质接触,并在脂蛋白脂肪酶辅助因子存在下与脂蛋白脂肪酶接触足以释放三酰基甘油的至少一部分脂肪酸的时间 ; 并确定在所述潜在活性物质的作用下抑制由脂蛋白脂肪酶的活性引起的脂肪酸的释放的能力,并评估抑制结果,其与在不存在 所测定的潜在活性物质与作为参考的已知抑制剂存在下获得的结果进行比较。 描述了化妆品和药物用途。