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62 条记录 (耗时 0.074 秒)

    Structured peptide processing
    11.
    发明授权
    Structured peptide processing 有权
    结构肽加工

    公开(公告)号：US08778844B2

    公开(公告)日：2014-07-15

    申请号：US13147257

    申请日：2010-02-04

    申请人： Gregory Paul Winter ,  Christian Heinis ,  Elise Bernard ,  David Loakes ,  Marina Vaysburd

    发明人： Gregory Paul Winter ,  Christian Heinis ,  Elise Bernard ,  David Loakes ,  Marina Vaysburd

    IPC分类号： C40B30/04

    CPC分类号： C12N15/1037 ,  A61K47/55 ,  A61K47/64 ,  A61K47/643 ,  C07K14/001 ,  C12N15/1044 ,  C12N15/1058 ,  C40B40/08 ,  C40B40/10 ,  C40B50/06 ,  G01N33/531

    摘要： The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.

    摘要翻译： 本发明涉及一种修饰一种或多种肽配体的方法，其包括在两个或更多个氨基酸残基处与分子支架共价连接的多肽，包括提供一个或多个肽配体的步骤，其中所述多肽包含两个或多个反应性基团 其形成与分子支架的共价键，以及至少一个环，其包含在两个所述反应性基团之间对位的两个或更多个氨基酸的序列; 将肽配体暴露于一种或多种蛋白酶; 并根据蛋白水解切割的程度对配体进行分选。

    PREVENTION OF AGGREGATION OF IMMUNOGLOBULIN LIGHT OR HEAVY CHAINS
    13.
    发明申请
    PREVENTION OF AGGREGATION OF IMMUNOGLOBULIN LIGHT OR HEAVY CHAINS 有权
    防止聚集免疫球蛋白轻链或重链

    公开(公告)号：US20100047237A1

    公开(公告)日：2010-02-25

    申请号：US12447908

    申请日：2007-10-26

    申请人： Rudolf M. T. DeWildt ,  Leo C. James ,  Philip C. Jones ,  Oliver Schon ,  Gregory Paul Winter

    发明人： Rudolf M. T. DeWildt ,  Leo C. James ,  Philip C. Jones ,  Oliver Schon ,  Gregory Paul Winter

    IPC分类号： A61K39/395 ,  C07K16/00 ,  C07K16/28 ,  C12N15/11 ,  C12N15/85 ,  C12N5/10 ,  C12P21/08

    CPC分类号： C07K16/22 ,  C07K16/241 ,  C07K16/42 ,  C07K2317/31 ,  C07K2317/567 ,  C07K2317/569

    摘要： The present invention provides an inhibitor of the aggregation of immunoglobulin chains (or immunoglobulin VL or CH1 domains). In preferred embodiments, the inhibitor comprises or consists of a polypeptide which comprises or consists of (a) an amino acid sequence corresponding to the amino acid sequence of the FR1 region of an immunoglobulin light chain variable domain, or part thereof which includes amino acid residue 12, (b) an amino acid sequence corresponding to the amino acid sequence of the immunoglobulin-binding domain of bacterial superantigen Protein L, or part thereof, and/or (c) an amino acid sequence corresponding to the amino acid sequence of the immunoglobulin-binding domain of streptococcal protein G (SpG), or part thereof, or a variant, fusion or derivative thereof, or a fusion of a said variant or derivative thereof which retains the ability of the parent polypeptide to inhibit aggregation of immunoglobulin chains, or domains thereof. The invention further provides a non-naturally occurring antibody, or antigen-binding fragment, fusion or derivative thereof comprising a light chain variable domain, or a fusion of said fragment or derivative, wherein the FR1 region of the light chain variable domain is modified (e.g., mutated) so as to inhibit, at least in part, the ability of the antibody, fragment, fusion or derivative to form aggregates. Most preferably, the light chain variable domain is modified at position 12 of the FR1 region by replacing the naturally occurring amino acid (e.g., serine in K light chains) with proline.

    摘要翻译： 本发明提供免疫球蛋白链（或免疫球蛋白VL或CH1结构域）聚集的抑制剂。 在优选的实施方案中，抑制剂包含多肽或由其组成，该多肽包含（a）对应于免疫球蛋白轻链可变结构域的FR1区的氨基酸序列或其部分的氨基酸序列，其部分包括氨基酸残基 12，（b）对应于细菌超抗原蛋白L的免疫球蛋白结合结构域的氨基酸序列或其部分的氨基酸序列，和/或（c）对应于免疫球蛋白的氨基酸序列的氨基酸序列 链球菌蛋白G（SpG）或其部分或其变体，融合物或衍生物或其所述变体或衍生物的融合物的结合结构域，其保留母体多肽抑制免疫球蛋白链的聚集的能力，或 其领域。 本发明还提供了非天然存在的抗体或其抗原结合片段，其融合物或衍生物，其包含轻链可变区或所述片段或衍生物的融合物，其中轻链可变结构域的FR1区域被修饰（ 例如突变），以至少部分地抑制抗体，片段，融合物或衍生物形成聚集体的能力。 最优选地，通过用脯氨酸代替天然存在的氨基酸（例如K轻链中的丝氨酸），将轻链可变结构域修饰在FR1区的12位。

    Altered antibodies
    17.
    发明授权
    Altered antibodies 失效
    改变抗体

    公开(公告)号：US06982321B2

    公开(公告)日：2006-01-03

    申请号：US10351748

    申请日：2003-01-24

    申请人： Gregory Paul Winter

    发明人： Gregory Paul Winter

    IPC分类号： C07K16/00

    CPC分类号： C07K16/464 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2319/00

    摘要： An altered antibody is produced by replacing the complementarity determining regions (CDRs) of a variable region of an immunoglobulin (Ig) with the CDRs from an Ig of different specificity, using recombinant DNA techniques. The gene coding sequence for producing the altered antibody may be produced by site-directed mutagenesis using long oligonucleotides or using gene synthesis.

    摘要翻译： 使用重组DNA技术，通过用来自不同特异性的Ig的CDR替代免疫球蛋白（Ig）的可变区的互补决定区（CDR）而产生改变的抗体。 用于产生改变的抗体的基因编码序列可以通过使用长寡核苷酸的定点诱变或使用基因合成产生。

    Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors
    18.
    发明授权
    Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors 失效
    单结构域配体，包含所述配体的受体，其制备方法和所述配体和受体的用途

    公开(公告)号：US06545142B1

    公开(公告)日：2003-04-08

    申请号：US09722364

    申请日：2000-11-28

    申请人： Gregory Paul Winter ,  Elizabeth Sally Ward ,  Detlef Güssow

    发明人： Gregory Paul Winter ,  Elizabeth Sally Ward ,  Detlef Güssow

    IPC分类号： C07H2104

    CPC分类号： C07K16/461 ,  A61K38/00 ,  C07K2317/24 ,  C12Q1/6876

    摘要： The present invention relates to single domain ligands derived from molecules in the immunoglobulin (Ig) superfamily, receptors comprising at least one such ligand, methods for cloning, amplifying and expressing DNA sequences encoding such ligands, preferably using the polymerase chain reaction, methods for the use of said DNA sequences in the production of Ig-type molecules and said ligands or receptors, and the use of said ligands or receptors in therapy, diagnosis or catalysis.

    摘要翻译： 本发明涉及衍生自免疫球蛋白（Ig）超家族中的分子的单结构域配体，包含至少一种这样的配体的受体，用于克隆，扩增和表达编码这种配体的DNA序列的方法，优选使用聚合酶链式反应， 在产生Ig型分子和所述配体或受体中使用所述DNA序列，以及所述配体或受体在治疗，诊断或催化中的用途。