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公开(公告)号：US20210163977A1
公开(公告)日：2021-06-03
申请号：US17079348
申请日：2020-10-23
Applicant: Monsanto Technology LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Shishieh Huang , Susan J. Johnson , Michael W. Petersen , David Walters , Paul S. Chomet
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
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公开(公告)号：US20180073037A1
公开(公告)日：2018-03-15
申请号：US15709291
申请日：2017-09-19
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R.I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
CPC classification number: C12N15/8286 , A01N63/02 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/162 , Y02A40/164
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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公开(公告)号：US09708620B2
公开(公告)日：2017-07-18
申请号：US14582303
申请日：2014-12-24
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Larry A. Gilbertson , Sara E. Heisel , Shihshieh Huang , Elysia K. Krieger , Thomas M. Malvar
IPC: C12N15/82 , C12N15/113
CPC classification number: C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US09701974B2
公开(公告)日：2017-07-11
申请号：US14521119
申请日：2014-10-22
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Susan J. Johnson
CPC classification number: C12N15/8216 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8218 , C12N15/8241
Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.
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公开(公告)号：US20170183684A1
公开(公告)日：2017-06-29
申请号：US15373377
申请日：2016-12-08
Applicant: Monsanto Technology LLC
Inventor： James A. Baum , Renata Bolognesi , Lex Evan Flagel , Gerrit Cornelis Segers , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
IPC: C12N15/82 , C12N15/113
CPC classification number: C12N15/8286 , A01N57/16 , C12N15/113 , C12N2310/14 , Y02A40/162
Abstract: The present invention provides methods for controlling invertebrate pest infestations, for instance in plants, and related compositions and polynucleotides useful in such methods. More specifically, the present invention provides polynucleotides and methods of use thereof for modifying the expression of genes in an invertebrate pest, for instance through RNA interference.
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Patent Agency Ranking
公开(公告)号：US20170029844A1
公开(公告)日：2017-02-02
申请号：US15217695
申请日：2016-07-22
Applicant: Monsanto Technology LLC
Inventor： Timothy K. Ball , Artem G. Evdokimov , Larry A. Gilbertson , Victor M. Guzov , Jeffrey A. Haas , Qing Huai , Sergey Ivashuta , Melissa M. Kemp , Yifei Kong , Thomas M. Malvar , Byron V. Olsen , Parthasarathy Ramaseshadri , Brian E. Weiner
CPC classification number: C12N15/8286 , A01N37/44 , A01N37/46 , C07K14/325 , Y02A40/162
Abstract: Pesticidal proteins are provided that combine two or more modes of action into a single molecule. These pesticidal proteins therefor exhibit greater efficacy and/or durability of resistance, for the purposes of pest control, and can be utilized for pest control by provision in the diet of a pest organism, or by topical application to crop plants and/or pests. Methods and compositions for producing and using such proteins are also provided.
Abstract translation: 提供了将两种或多种作用模式组合成单个分子的杀虫蛋白质。 这些杀虫蛋白为了有害生物控制的目的而具有较高的抗性和/或耐久性，并且可以通过在有害生物的饮食中提供病虫害防治，或通过局部应用于作物植物和/或害虫来利用。 还提供了用于生产和使用这些蛋白质的方法和组合物。
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公开(公告)号：US20140338010A1
公开(公告)日：2014-11-13
申请号：US14337932
申请日：2014-07-22
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Shihshieh Huang , Thomas Malvar
IPC: C12N15/82
CPC classification number: C12N15/8213 , C12N15/63 , C12N15/8217 , C12N15/8218 , C12N15/8243
Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
Abstract translation: 用于植物和植物细胞的重组DNA构建体具有位点特异性重组位点，其允许通过过表达或抑制内源基因来评估表型和作用模式。 在一方面，单个DNA构建体可以通过重组酶（例如Cre重组酶）对具有lox重组位点的构建体的作用在过度表达和抑制之间切换。 其他有用的重组系统包括Flp / frt系统，R / Rs系统，Dre / rox系统和GIN / gix系统。
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公开(公告)号：US12157893B2
公开(公告)日：2024-12-03
申请号：US17523755
申请日：2021-11-10
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： John Bradley , Larry A. Gilbertson , Ervin Nagy , Christine Shyu , Peizhen Yang , Xudong Ye
IPC: C12N15/82
Abstract: The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells.
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公开(公告)号：US11629357B2
公开(公告)日：2023-04-18
申请号：US17079348
申请日：2020-10-23
Applicant: Monsanto Technology LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Shihshieh Huang , Susan J. Johnson , Michael W. Petersen , David Walters , Paul S. Chomet
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
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公开(公告)号：US11162109B2
公开(公告)日：2021-11-02
申请号：US16221346
申请日：2018-12-14
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Larry A. Gilbertson , Jon Lamb , Jason Ward , Jianping Xu
IPC: C12N15/82
Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.
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